UniProtKB - P39518 (LCF2_YEAST)
Protein
Long-chain-fatty-acid--CoA ligase 2
Gene
FAA2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Esterification, concomitant with transport, of endogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Preferentially acts on C9:0-C13:0 fatty acids although C7:0-C17:0 fatty acids are tolerated.
Miscellaneous
The FAM1-1 suppressor mutant has a single base mutation that creates a new initiator methionine and adds 18 residues at the N-terminus of the protein. This extra sequence acts as a transit peptide and the resulting protein is imported in the mitochondria.
Present with 358 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:6.2.1.3
Cofactori
Mg2+By similarity
Temperature dependencei
Optimum temperature is 25 degrees Celsius.
GO - Molecular functioni
- acyl-CoA ligase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- decanoate-CoA ligase activity Source: UniProtKB-EC
- long-chain fatty acid-CoA ligase activity Source: SGD
- medium-chain fatty acid-CoA ligase activity Source: SGD
- very long-chain fatty acid-CoA ligase activity Source: SGD
GO - Biological processi
- fatty acid beta-oxidation Source: UniProtKB
- fatty-acyl-CoA transport Source: UniProtKB
- long-chain fatty acid import into peroxisome Source: UniProtKB
- long-chain fatty acid metabolic process Source: SGD
- very long-chain fatty acid catabolic process Source: UniProtKB
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:YER015W-MONOMER |
BRENDAi | 6.2.1.3, 984 |
Reactomei | R-SCE-75876, Synthesis of very long-chain fatty acyl-CoAs |
Protein family/group databases
TCDBi | 4.C.1.1.15, the fatty acid transporter (fat) family |
Chemistry databases
SwissLipidsi | SLP:000001032 |
Names & Taxonomyi
Protein namesi | Recommended name: Long-chain-fatty-acid--CoA ligase 2 (EC:6.2.1.3)Alternative name(s): Fatty acid activator 2 Long-chain acyl-CoA synthetase 2 |
Gene namesi | Name:FAA2 Synonyms:FAM1 Ordered Locus Names:YER015W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YER015W |
SGDi | S000000817, FAA2 |
Subcellular locationi
Mitochondrion
Other locations
Note: The FAM1-1 mutant is imported in the mitochondria.
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Mitochondrion
- mitochondrion Source: SGD
Peroxisome
- peroxisome Source: SGD
Other locations
- membrane Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 | M → MWKNAGYKKRIRTNLFRNM in FAM1-1. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193120 | 1 – 744 | Long-chain-fatty-acid--CoA ligase 2Add BLAST | 744 |
Proteomic databases
MaxQBi | P39518 |
PaxDbi | P39518 |
PRIDEi | P39518 |
PTM databases
iPTMneti | P39518 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 36747, 39 interactors |
DIPi | DIP-6565N |
IntActi | P39518, 5 interactors |
MINTi | P39518 |
STRINGi | 4932.YER015W |
Miscellaneous databases
RNActi | P39518, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | KOG1256, Eukaryota |
GeneTreei | ENSGT00960000189179 |
HOGENOMi | CLU_000022_45_4_1 |
InParanoidi | P39518 |
OMAi | PKYVENK |
Family and domain databases
Gene3Di | 3.40.50.12780, 2 hits |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
P39518-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAPDYALTD LIESDPRFES LKTRLAGYTK GSDEYIEELY SQLPLTSYPR
60 70 80 90 100
YKTFLKKQAV AISNPDNEAG FSSIYRSSLS SENLVSCVDK NLRTAYDHFM
110 120 130 140 150
FSARRWPQRD CLGSRPIDKA TGTWEETFRF ESYSTVSKRC HNIGSGILSL
160 170 180 190 200
VNTKRKRPLE ANDFVVAILS HNNPEWILTD LACQAYSLTN TALYETLGPN
210 220 230 240 250
TSEYILNLTE APILIFAKSN MYHVLKMVPD MKFVNTLVCM DELTHDELRM
260 270 280 290 300
LNESLLPVKC NSLNEKITFF SLEQVEQVGC FNKIPAIPPT PDSLYTISFT
310 320 330 340 350
SGTTGLPKGV EMSHRNIASG IAFAFSTFRI PPDKRNQQLY DMCFLPLAHI
360 370 380 390 400
FERMVIAYDL AIGFGIGFLH KPDPTVLVED LKILKPYAVA LVPRILTRFE
410 420 430 440 450
AGIKNALDKS TVQRNVANTI LDSKSARFTA RGGPDKSIMN FLVYHRVLID
460 470 480 490 500
KIRDSLGLSN NSFIITGSAP ISKDTLLFLR SALDIGIRQG YGLTETFAGV
510 520 530 540 550
CLSEPFEKDV GSCGAIGISA ECRLKSVPEM GYHADKDLKG ELQIRGPQVF
560 570 580 590 600
ERYFKNPNET SKAVDQDGWF STGDVAFIDG KGRISVIDRV KNFFKLAHGE
610 620 630 640 650
YIAPEKIENI YLSSCPYITQ IFVFGDPLKT FLVGIVGVDV DAAQPILAAK
660 670 680 690 700
HPEVKTWTKE VLVENLNRNK KLRKEFLNKI NKCTDGLQGF EKLHNIKVGL
710 720 730 740
EPLTLEDDVV TPTFKIKRAK ASKFFKDTLD QLYAEGSLVK TEKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77783 Genomic DNA Translation: CAA54817.1 X82364 Genomic DNA Translation: CAA57780.1 U18778 Genomic DNA Translation: AAB64548.1 BK006939 Genomic DNA Translation: DAA07667.1 |
PIRi | A54901 |
RefSeqi | NP_010931.3, NM_001178906.3 |
Genome annotation databases
EnsemblFungii | YER015W_mRNA; YER015W; YER015W |
GeneIDi | 856734 |
KEGGi | sce:YER015W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X77783 Genomic DNA Translation: CAA54817.1 X82364 Genomic DNA Translation: CAA57780.1 U18778 Genomic DNA Translation: AAB64548.1 BK006939 Genomic DNA Translation: DAA07667.1 |
PIRi | A54901 |
RefSeqi | NP_010931.3, NM_001178906.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36747, 39 interactors |
DIPi | DIP-6565N |
IntActi | P39518, 5 interactors |
MINTi | P39518 |
STRINGi | 4932.YER015W |
Chemistry databases
SwissLipidsi | SLP:000001032 |
Protein family/group databases
TCDBi | 4.C.1.1.15, the fatty acid transporter (fat) family |
PTM databases
iPTMneti | P39518 |
Proteomic databases
MaxQBi | P39518 |
PaxDbi | P39518 |
PRIDEi | P39518 |
Genome annotation databases
EnsemblFungii | YER015W_mRNA; YER015W; YER015W |
GeneIDi | 856734 |
KEGGi | sce:YER015W |
Organism-specific databases
EuPathDBi | FungiDB:YER015W |
SGDi | S000000817, FAA2 |
Phylogenomic databases
eggNOGi | KOG1256, Eukaryota |
GeneTreei | ENSGT00960000189179 |
HOGENOMi | CLU_000022_45_4_1 |
InParanoidi | P39518 |
OMAi | PKYVENK |
Enzyme and pathway databases
BioCyci | MetaCyc:YER015W-MONOMER |
BRENDAi | 6.2.1.3, 984 |
Reactomei | R-SCE-75876, Synthesis of very long-chain fatty acyl-CoAs |
Miscellaneous databases
PROi | PR:P39518 |
RNActi | P39518, protein |
Family and domain databases
Gene3Di | 3.40.50.12780, 2 hits |
InterProi | View protein in InterPro IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LCF2_YEAST | |
Accessioni | P39518Primary (citable) accession number: P39518 Secondary accession number(s): D3DLR3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD