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Protein

ATP-dependent RNA helicase DHH1

Gene

DHH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase involved in mRNA turnover, and more specifically in mRNA decapping by activating the decapping enzyme DCP1 (PubMed:11780629, PubMed:12032091, PubMed:11696541, PubMed:12730603, PubMed:15703442. PubMed:15706350). Is involved in G1/S DNA-damage checkpoint recovery, probably through the regulation of the translational status of a subset of mRNAs (PubMed:15166134). May also have a role in translation and mRNA nuclear export (PubMed:12930949). Required for sporulation (PubMed:12930949). Blocks autophagy in nutrient-rich conditions by, at least partly, binding and repressing the expression of a set of ATG genes, including ATG3, ATG7, ATG8, ATG19, ATG20, ATG22 and SNX4/ATG24 (PubMed:26098573).9 Publications

Miscellaneous

Present with 42900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi90 – 97ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • chromatin binding Source: SGD
  • mRNA binding Source: SGD
  • translation regulator activity, nucleic acid binding Source: SGD

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processmRNA processing, mRNA transport, Translation regulation, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29554-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DHH1Curated (EC:3.6.4.13Curated)
Alternative name(s):
DExD/H-box helicase 11 Publication
Gene namesi
Name:DHH11 Publication
Ordered Locus Names:YDL160CImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL160C
SGDiS000002319 DHH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Leads to an increased in autophagy flux (PubMed:26098573). Causes an accumulation of ATG3, ATG7, ATG8, ATG19, ATG20, ATG22 and SNX4/ATG24 transcripts in nutrient-replete conditions (PubMed:26098573).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi89R → A: Leads to mRNA turnover defect and no growth at 37 degrees Celsius; when associated with A-91. Impairs RNA binding in vitro. 1 Publication1
Mutagenesisi91K → A: Leads to mRNA turnover defect and no growth at 37 degrees Celsius; when associated with A-89. Impairs RNA binding in vitro. 1 Publication1
Mutagenesisi195D → A: Leads to mRNA turnover defect and no growth at 37 degrees Celsius. 1 Publication1
Mutagenesisi196E → A: Leads to mRNA turnover defect and no growth at 37 degrees Celsius. 1 Publication1
Mutagenesisi345R → A: Leads to mRNA turnover defect and no growth at 37 degrees Celsius. Impairs RNA binding in vitro. 1 Publication1
Mutagenesisi346G → A: Leads to mRNA turnover defect and no growth at 37 degrees Celsius. Impairs RNA binding in vitro. 1 Publication1
Mutagenesisi369H → A: Leads to mRNA turnover defect and no growth at 37 degrees Celsius. 1 Publication1
Mutagenesisi370R → A: Leads to mRNA turnover defect and no growth at 37 degrees Celsius. Impairs RNA binding in vitro. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550441 – 506ATP-dependent RNA helicase DHH1Add BLAST506

Proteomic databases

MaxQBiP39517
PaxDbiP39517
PRIDEiP39517

PTM databases

iPTMnetiP39517

Interactioni

Subunit structurei

Associated with the CCR4-NOT complex and possibly other big complexes (PubMed:9504907, PubMed:12930949). Interacts with CDC39/NOT1 (PubMed:11696541). Interacts with DCP1, LSM1, and POP2 (PubMed:9504907, PubMed:11780629). Interacts with IGO1 (PubMed:20471941). Interacts with PAT1 and with KEM1, the major 5'-3' exonuclease (PubMed:11780629, PubMed:12032091).6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi31903, 3619 interactors
DIPiDIP-1243N
IntActiP39517, 41 interactors
MINTiP39517
STRINGi4932.YDL160C

Structurei

Secondary structure

1506
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP39517
SMRiP39517
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39517

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini77 – 247Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini257 – 417Helicase C-terminalPROSITE-ProRule annotationAdd BLAST161

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi46 – 74Q motifAdd BLAST29
Motifi195 – 198DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi434 – 505Gln-richAdd BLAST72

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00900000141067
HOGENOMiHOG000268797
InParanoidiP39517
KOiK12614
OMAiDWNLMSS
OrthoDBiEOG092C2B1L

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

P39517-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSINNNFNT NNNSNTDLDR DWKTALNIPK KDTRPQTDDV LNTKGNTFED
60 70 80 90 100
FYLKRELLMG IFEAGFEKPS PIQEEAIPVA ITGRDILARA KNGTGKTAAF
110 120 130 140 150
VIPTLEKVKP KLNKIQALIM VPTRELALQT SQVVRTLGKH CGISCMVTTG
160 170 180 190 200
GTNLRDDILR LNETVHILVG TPGRVLDLAS RKVADLSDCS LFIMDEADKM
210 220 230 240 250
LSRDFKTIIE QILSFLPPTH QSLLFSATFP LTVKEFMVKH LHKPYEINLM
260 270 280 290 300
EELTLKGITQ YYAFVEERQK LHCLNTLFSK LQINQAIIFC NSTNRVELLA
310 320 330 340 350
KKITDLGYSC YYSHARMKQQ ERNKVFHEFR QGKVRTLVCS DLLTRGIDIQ
360 370 380 390 400
AVNVVINFDF PKTAETYLHR IGRSGRFGHL GLAINLINWN DRFNLYKIEQ
410 420 430 440 450
ELGTEIAAIP ATIDKSLYVA ENDETVPVPF PIEQQSYHQQ AIPQQQLPSQ
460 470 480 490 500
QQFAIPPQQH HPQFMVPPSH QQQQAYPPPQ MPSQQGYPPQ QEHFMAMPPG

QSQPQY
Length:506
Mass (Da):57,544
Last modified:February 1, 1995 - v1
Checksum:i062CFA56DF6F2CEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66057 Genomic DNA Translation: CAA46853.1
Z67750 Genomic DNA Translation: CAA91586.1
Z74208 Genomic DNA Translation: CAA98734.1
BK006938 Genomic DNA Translation: DAA11700.1
PIRiS31229
RefSeqiNP_010121.1, NM_001180220.1

Genome annotation databases

EnsemblFungiiYDL160C; YDL160C; YDL160C
GeneIDi851394
KEGGisce:YDL160C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66057 Genomic DNA Translation: CAA46853.1
Z67750 Genomic DNA Translation: CAA91586.1
Z74208 Genomic DNA Translation: CAA98734.1
BK006938 Genomic DNA Translation: DAA11700.1
PIRiS31229
RefSeqiNP_010121.1, NM_001180220.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S2MX-ray2.10A31-425[»]
4BRUX-ray3.24A46-422[»]
4BRWX-ray2.80A46-422[»]
ProteinModelPortaliP39517
SMRiP39517
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31903, 3619 interactors
DIPiDIP-1243N
IntActiP39517, 41 interactors
MINTiP39517
STRINGi4932.YDL160C

PTM databases

iPTMnetiP39517

Proteomic databases

MaxQBiP39517
PaxDbiP39517
PRIDEiP39517

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL160C; YDL160C; YDL160C
GeneIDi851394
KEGGisce:YDL160C

Organism-specific databases

EuPathDBiFungiDB:YDL160C
SGDiS000002319 DHH1

Phylogenomic databases

GeneTreeiENSGT00900000141067
HOGENOMiHOG000268797
InParanoidiP39517
KOiK12614
OMAiDWNLMSS
OrthoDBiEOG092C2B1L

Enzyme and pathway databases

BioCyciYEAST:G3O-29554-MONOMER

Miscellaneous databases

EvolutionaryTraceiP39517
PROiPR:P39517

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDHH1_YEAST
AccessioniPrimary (citable) accession number: P39517
Secondary accession number(s): D6VRJ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 12, 2018
This is version 180 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names
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Main funding by: National Institutes of Health

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