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Entry version 196 (22 Apr 2020)
Sequence version 2 (27 Jul 2011)
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Protein

Tight junction protein ZO-1

Gene

Tjp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells (By similarity). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (By similarity). With Tjp2 and TJjp3, participates to the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus (By similarity).By similarity

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionCalmodulin-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-MMU-191650 Regulation of gap junction activity
R-MMU-2028269 Signaling by Hippo
R-MMU-351906 Apoptotic cleavage of cell adhesion proteins

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Tight junction protein ZO-1
Alternative name(s):
Tight junction protein 1
Zona occludens protein 1
Zonula occludens protein 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Tjp1
Synonyms:Zo1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 7

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...
MGIi
MGI:98759 Tjp1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Gap junction, Membrane, Tight junction

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000945411 – 1745Tight junction protein ZO-1Add BLAST1745

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei125PhosphoserineCombined sources1
Modified residuei132PhosphotyrosineBy similarity1
Modified residuei175PhosphoserineBy similarity1
Modified residuei178PhosphoserineBy similarity1
Modified residuei179PhosphoserineBy similarity1
Modified residuei185PhosphothreonineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei241PhosphoserineCombined sources1
Modified residuei267PhosphothreonineBy similarity1
Modified residuei275PhosphoserineBy similarity1
Modified residuei277PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1
Modified residuei284PhosphoserineCombined sources1
Modified residuei290PhosphoserineBy similarity1
Modified residuei294PhosphoserineCombined sources1
Modified residuei297PhosphoserineBy similarity1
Modified residuei300PhosphoserineBy similarity1
Modified residuei323PhosphoserineCombined sources1
Modified residuei329PhosphoserineBy similarity1
Modified residuei334PhosphoserineBy similarity1
Modified residuei337PhosphoserineBy similarity1
Modified residuei353PhosphoserineCombined sources1
Modified residuei354PhosphothreonineBy similarity1
Modified residuei617PhosphoserineCombined sources1
Modified residuei622PhosphoserineBy similarity1
Modified residuei809PhosphothreonineBy similarity1
Modified residuei810PhosphoserineCombined sources1
Modified residuei821PhosphoserineCombined sources1
Modified residuei822PhosphotyrosineCombined sources1
Modified residuei824PhosphoserineCombined sources1
Modified residuei828PhosphoserineBy similarity1
Modified residuei837PhosphoserineBy similarity1
Modified residuei846PhosphothreonineBy similarity1
Modified residuei848PhosphothreonineCombined sources1
Modified residuei854PhosphothreonineBy similarity1
Modified residuei861PhosphothreonineBy similarity1
Modified residuei868PhosphothreonineCombined sources1
Modified residuei912PhosphoserineCombined sources1
Modified residuei1071PhosphoserineCombined sources1
Modified residuei1138PhosphoserineCombined sources1
Modified residuei1139PhosphotyrosineCombined sources1
Modified residuei1164PhosphotyrosineCombined sources1
Modified residuei1353PhosphotyrosineCombined sources1
Modified residuei1365PhosphoserineBy similarity1
Modified residuei1411PhosphoserineBy similarity1
Modified residuei1542PhosphoserineCombined sources1
Modified residuei1614PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF) (By similarity). This response is dependent on an intact actin microfilament system (By similarity). Dephosphorylated by PTPRJ (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P39447

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P39447

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P39447

PeptideAtlas

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PeptideAtlasi
P39447

PRoteomics IDEntifications database

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PRIDEi
P39447

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P39447

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
P39447

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSMUSG00000030516 Expressed in brain blood vessel and 305 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
P39447 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
P39447 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer, and heterodimer with TJP2 and TJP3.

Interacts with OCLN, CALM, claudins, CGN/cingulin, CXADR, GJD3 and UBN1 (PubMed:10601346).

Interacts (via ZU5 domain) with CDC42BPB (By similarity).

Interacts (via PDZ domain) with GJA1 (By similarity).

Interacts (via PDZ domains) with ANKRD2 (By similarity).

Interacts with BVES (via the C-terminus cytoplasmic tail) (PubMed:16188940).

Interacts with GJA12 and KIRREL1 (PubMed:12424224, PubMed:15183511).

Interacts with HSPA4 (By similarity).

Interacts (via ZU5 domain) with MYZAP (PubMed:20093627).

Interacts with DLL1 (PubMed:24715457).

Interacts with USP53 (via the C-terminal region) (PubMed:26609154).

Interacts with DNMBP (via C-terminal domain); required for the apical cell-cell junction localization of DNMBP (By similarity).

By similarity7 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
204209, 12 interactors

Database of interacting proteins

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DIPi
DIP-30946N

Protein interaction database and analysis system

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IntActi
P39447, 24 interactors

Molecular INTeraction database

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MINTi
P39447

STRING: functional protein association networks

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STRINGi
10090.ENSMUSP00000099652

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

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RNActi
P39447 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11745
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
P39447

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

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PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

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EvolutionaryTracei
P39447

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini23 – 110PDZ 1PROSITE-ProRule annotationAdd BLAST88
Domaini186 – 264PDZ 2PROSITE-ProRule annotationAdd BLAST79
Domaini421 – 502PDZ 3PROSITE-ProRule annotationAdd BLAST82
Domaini516 – 584SH3PROSITE-ProRule annotationAdd BLAST69
Domaini610 – 791Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST182
Domaini1631 – 1745ZU5PROSITE-ProRule annotationAdd BLAST115

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni633 – 876Occludin (OCLN)-binding regionBy similarityAdd BLAST244
Regioni1150 – 1370Actin-binding region (ABR)By similarityAdd BLAST221

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi1242 – 1247Poly-Pro6
Compositional biasi1424 – 1430Poly-Pro7

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The 244-aa domain between residues 633 and 876 is the primary occludin (Ocln)-binding site and is required for stable association with the tight junction (By similarity).By similarity
The C-terminal region (residues 1151-1372) is an actin-binding region (ABR) that interacts directly with F-actin and plays an important role in the localization of Tjp1 at junctions (By similarity). The ABR is also required for the localization to puncta at the free edge of cells before initiation of cell-cell contact (By similarity). The ABR is also necessary for Tjp1 recruitment to podosomes (By similarity).By similarity
The second PDZ domain (PDZ2) mediates homodimerization and heterodimerization with Tjp2 and Tjp3 (By similarity). PDZ2 domain also mediates interaction with Gja12 (PubMed:15183511).By similarity1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the MAGUK family.Curated

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3580 Eukaryota
ENOG410XQP3 LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00940000155164

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
P39447

KEGG Orthology (KO)

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KOi
K05701

Database of Orthologous Groups

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OrthoDBi
1175136at2759

TreeFam database of animal gene trees

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TreeFami
TF315957

Family and domain databases

Conserved Domains Database

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CDDi
cd12026 SH3_ZO-1, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR027417 P-loop_NTPase
IPR001478 PDZ
IPR036034 PDZ_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR005417 ZO
IPR005418 ZO-1
IPR035597 ZO-1_SH3
IPR000906 ZU5_dom

The PANTHER Classification System

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PANTHERi
PTHR13865:SF25 PTHR13865:SF25, 1 hit

Pfam protein domain database

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Pfami
View protein in Pfam
PF00625 Guanylate_kin, 1 hit
PF00595 PDZ, 3 hits
PF07653 SH3_2, 1 hit
PF00791 ZU5, 1 hit

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR01597 ZONOCCLUDNS
PR01598 ZONOCCLUDNS1

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00072 GuKc, 1 hit
SM00228 PDZ, 3 hits
SM00218 ZU5, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50044 SSF50044, 1 hit
SSF50156 SSF50156, 3 hits
SSF52540 SSF52540, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50052 GUANYLATE_KINASE_2, 1 hit
PS50106 PDZ, 3 hits
PS50002 SH3, 1 hit
PS51145 ZU5, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

P39447-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSARAAAAKS TAMEETAIWE QHTVTLHRAP GFGFGIAISG GRDNPHFQSG
60 70 80 90 100
ETSIVISDVL KGGPAEGQLQ ENDRVAMVNG VSMDNVEHAF AVQQLRKSGK
110 120 130 140 150
NAKITIRRKK KVQIPVSHPD PEPVSDNEDD SYDEEVHDPR AGRGALANRR
160 170 180 190 200
SEKSWARDRS ASRERSLSPR SDRRSVASSQ PAKPTKVTLV KSRKNEEYGL
210 220 230 240 250
RLASHIFVKE ISQDSLAARD GNIQEGDVVL KINGTVTENM SLTDAKTLIE
260 270 280 290 300
RSKGKLKMVV QRDERATLLN VPDLSDSIHS ANASERDDIS EIQSLASDHS
310 320 330 340 350
GRSHDRPPRR SQSRSPDQRS EPSDHSTQSP QQPSNGSLRS REEERMSKPG
360 370 380 390 400
AISTPVKHVD DHPPKAVEEV TVEKNEKQTP TLPEPKPVYA QVGQPDVDLP
410 420 430 440 450
VSPSDGALPN SAHEDGILRP SMKLVKFRKG DSVGLRLAGG NDVGIFVAGV
460 470 480 490 500
LEDSPAAKEG LEEGDQILRV NNVDFTNIIR EEAVLFLLDL PKGEEVTILA
510 520 530 540 550
QKKKDVYRRI VESDVGDSFY IRTHFEYEKE SPYGLSFNKG EVFRVVDTLY
560 570 580 590 600
NGKLGSWLAI RIGKNHKEVE RGIIPNKNRA EQLASVQYTL PKTAGGDRAD
610 620 630 640 650
FWRFRGLRSS KRNLRKSRED LSAQPVQTKF PAYERVVLRE AGFLRPVTIF
660 670 680 690 700
GPIADVAREK LAREEPDIYQ IAKSEPRDAG TDHRSSGIIR LHTIKQIIDQ
710 720 730 740 750
DKHALLDVTP NAVDRLNYAQ WYPIVVFLNP DSKQGVKTMR MRLCPESRKS
760 770 780 790 800
ARKLYERSHK LRKNNHHLFT TTINLNSMND GWYGALKEAI QQQQNQLVWV
810 820 830 840 850
SEGKADGATS DDLDLHDDRL SYLSAPGSEY SMYSTDSRHT SDYEDTDTEG
860 870 880 890 900
GAYTDQELDE TLNDEVGTPP ESAITRSSEP VREDSSGMHH ENQTYPPYSP
910 920 930 940 950
QAQPQAIHRI DSPGLKPASQ QKAEASSPVP YLSPETTPAS SASAVNHNVS
960 970 980 990 1000
VTNVSLEEPA PAPPTSHASQ PGCLGAPSAE AAHVVLRGEG PPLPPHADPA
1010 1020 1030 1040 1050
KVYRKEPYSE EMMRQNHILK QPALGHPGQR PDKEPNLAYE PQLPYIEKQA
1060 1070 1080 1090 1100
SRDLEQPSYR YEVSSYTDQF SRNYDHRLRF EDRIPTYEDQ WSYYDDKQPY
1110 1120 1130 1140 1150
QPRPFENQHP RDLDSRQHPE EASERGYFQR FEEPAPLSYD SRTRYEQLPR
1160 1170 1180 1190 1200
TSTLRHEEQP APAYEVHNRY RPEAQPYSST GPKSSEPKQY FDQYPRSYEQ
1210 1220 1230 1240 1250
VPPPGFTSKT GHYEPLHGAA VVPPLIPSSQ QKPEVLPSAT KPQPPPPTLT
1260 1270 1280 1290 1300
EEEEDPAMKP QSVLTRVKMF ENKRSASLEN KKDVNDTASF KPPEVASKPP
1310 1320 1330 1340 1350
GASLAGPKPV PQSQFSEHDK TLYRLPEPQK PQVKPPEDIV RSNHYDPEED
1360 1370 1380 1390 1400
EEYYRKQLSY FDRRSFESKP SAHLPAGHHS EPAKPVHSQS QPNFSSYSSK
1410 1420 1430 1440 1450
GKPETDAVDR SFSEKRYDPA QATPPPPPLP SQYSQPAPPL SSSSLHIHSK
1460 1470 1480 1490 1500
GAQGEGNSVS LDFQNSYMSK PDPPPSQSKP ATFRPPTRED PPQTFYPQKS
1510 1520 1530 1540 1550
FPDKAPVNGA EQTQKTITPV YNRFTPKPYT SSARPFERKF ESPKFNHNLL
1560 1570 1580 1590 1600
PSETVHKPEL SSKTPTSPKT LMKAHSSTQP PEFDSGVETF SVHTDKPKYQ
1610 1620 1630 1640 1650
MNNISTMPKA VPVSPSAVEE DEDEDGHTVV ATARGIFNSN GGVLSSIETG
1660 1670 1680 1690 1700
VSIIIPQGAI PEGIEQEIYF KVCRDNSILP PLDKEKGETL LSPLVMCGPH
1710 1720 1730 1740
GLKFLKPVEL RLPHCDPKTW QNKCLPGDPN YLVGANCVSV LIDHF
Length:1,745
Mass (Da):194,742
Last modified:July 27, 2011 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i002B96F5A998B5CD
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B9EHJ3B9EHJ3_MOUSE
Tight junction protein ZO-1
Tjp1
1,685Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0U1RPW2A0A0U1RPW2_MOUSE
Tight junction protein ZO-1
Tjp1
1,128Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A0U1RPB2A0A0U1RPB2_MOUSE
Tight junction protein ZO-1
Tjp1
171Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti202L → P in BAA03274 (PubMed:8486731).Curated1
Sequence conflicti222N → D in BAA03274 (PubMed:8486731).Curated1
Sequence conflicti676P → L in BAA03274 (PubMed:8486731).Curated1
Sequence conflicti985V → G in BAA03274 (PubMed:8486731).Curated1
Sequence conflicti1237P → R in BAA03274 (PubMed:8486731).Curated1
Sequence conflicti1395S → P in BAA03274 (PubMed:8486731).Curated1
Sequence conflicti1584D → G in BAA03274 (PubMed:8486731).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
D14340 mRNA Translation: BAA03274.1
AC122222 Genomic DNA No translation available.
AC131741 Genomic DNA No translation available.

The Consensus CDS (CCDS) project

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CCDSi
CCDS21338.1

Protein sequence database of the Protein Information Resource

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PIRi
A46431

NCBI Reference Sequences

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RefSeqi
NP_033412.2, NM_009386.2

Genome annotation databases

Ensembl eukaryotic genome annotation project

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Ensembli
ENSMUST00000102592; ENSMUSP00000099652; ENSMUSG00000030516

Database of genes from NCBI RefSeq genomes

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GeneIDi
21872

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
mmu:21872

UCSC genome browser

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UCSCi
uc009hgp.2 mouse

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14340 mRNA Translation: BAA03274.1
AC122222 Genomic DNA No translation available.
AC131741 Genomic DNA No translation available.
CCDSiCCDS21338.1
PIRiA46431
RefSeqiNP_033412.2, NM_009386.2

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RRMNMR-A18-110[»]
SMRiP39447
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi204209, 12 interactors
DIPiDIP-30946N
IntActiP39447, 24 interactors
MINTiP39447
STRINGi10090.ENSMUSP00000099652

PTM databases

iPTMnetiP39447
PhosphoSitePlusiP39447

Proteomic databases

jPOSTiP39447
MaxQBiP39447
PaxDbiP39447
PeptideAtlasiP39447
PRIDEiP39447

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
783 405 antibodies

Genome annotation databases

EnsembliENSMUST00000102592; ENSMUSP00000099652; ENSMUSG00000030516
GeneIDi21872
KEGGimmu:21872
UCSCiuc009hgp.2 mouse

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
7082
MGIiMGI:98759 Tjp1

Phylogenomic databases

eggNOGiKOG3580 Eukaryota
ENOG410XQP3 LUCA
GeneTreeiENSGT00940000155164
InParanoidiP39447
KOiK05701
OrthoDBi1175136at2759
TreeFamiTF315957

Enzyme and pathway databases

ReactomeiR-MMU-191650 Regulation of gap junction activity
R-MMU-2028269 Signaling by Hippo
R-MMU-351906 Apoptotic cleavage of cell adhesion proteins

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

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ChiTaRSi
Tjp1 mouse
EvolutionaryTraceiP39447

Protein Ontology

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PROi
PR:P39447
RNActiP39447 protein

The Stanford Online Universal Resource for Clones and ESTs

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SOURCEi
Search...

Gene expression databases

BgeeiENSMUSG00000030516 Expressed in brain blood vessel and 305 other tissues
ExpressionAtlasiP39447 baseline and differential
GenevisibleiP39447 MM

Family and domain databases

CDDicd12026 SH3_ZO-1, 1 hit
InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR027417 P-loop_NTPase
IPR001478 PDZ
IPR036034 PDZ_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR005417 ZO
IPR005418 ZO-1
IPR035597 ZO-1_SH3
IPR000906 ZU5_dom
PANTHERiPTHR13865:SF25 PTHR13865:SF25, 1 hit
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
PF00595 PDZ, 3 hits
PF07653 SH3_2, 1 hit
PF00791 ZU5, 1 hit
PRINTSiPR01597 ZONOCCLUDNS
PR01598 ZONOCCLUDNS1
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SM00228 PDZ, 3 hits
SM00218 ZU5, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF50156 SSF50156, 3 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50052 GUANYLATE_KINASE_2, 1 hit
PS50106 PDZ, 3 hits
PS50002 SH3, 1 hit
PS51145 ZU5, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiZO1_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P39447
Secondary accession number(s): E9QK00
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: April 22, 2020
This is version 196 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
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