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Protein

TNF receptor-associated factor 1

Gene

Traf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity).By similarity2 Publications

Caution

Lacks a RING domain and has therefore no E3 ubiquitin-protein ligase activity by itself.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-MMU-5357905 Regulation of TNFR1 signaling
R-MMU-5357956 TNFR1-induced NFkappaB signaling pathway

Protein family/group databases

MoonDBiP39428 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
TNF receptor-associated factor 1
Gene namesi
Name:Traf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:101836 Traf1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice have normal B-cell proliferation and antibody response, but increased T-cell proliferation in response to CD3 signaling. Their T-cells show enhanced activation of JNK and NF-kappa-B. Mice are highly susceptible to TNF-induced skin necrosis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi139S → A: Loss of phosphorylation site. Reduces global phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563981 – 409TNF receptor-associated factor 1Add BLAST409

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139Phosphoserine1 Publication1
Cross-linki178Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki186Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Polyubiquitinated by BIRC2 and/or BIRC3, leading to its subsequent proteasomal degradation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei156 – 157Cleavage; by CASP8By similarity2

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP39428
MaxQBiP39428
PaxDbiP39428
PeptideAtlasiP39428
PRIDEiP39428

PTM databases

iPTMnetiP39428
PhosphoSitePlusiP39428

Expressioni

Gene expression databases

BgeeiENSMUSG00000026875 Expressed in 94 organ(s), highest expression level in subcutaneous adipose tissue
CleanExiMM_TRAF1
ExpressionAtlasiP39428 baseline and differential
GenevisibleiP39428 MM

Interactioni

Subunit structurei

Homotrimer (By similarity). Heterotrimer with TRAF2 (PubMed:8069916). Interacts with TNFRSF1A/TNFR1, TNFRSF1B/TNFR2, TNFRSF4, TNFRSF5/CD40, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13C, TNFRSF18/AITR, TNFRSF17/BCMA, TNFRSF19/TROY, TNFRSF19L/RELT, XEDAR, EDAR, Epstein-Barr virus BNFL1/LMP-1, TANK/ITRAF, TRAIP and RIPK2 (PubMed:8069916, PubMed:9104814, PubMed:18429822). Interacts with BIRC2 and BIRC3 N-terminus; a single BIRC2 or BIRC3 molecule interacts with a heterotrimer formed by TRAF1 and TRAF2. Interacts with MAP3K14 (By similarity). Interacts with NFATC2IP, TRAFD1 and with HIVEP3 (PubMed:11804591, PubMed:16352630, PubMed:18849341). Interacts with GPS2 (PubMed:22424771).By similarity7 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204302, 6 interactors
DIPiDIP-260N
IntActiP39428, 35 interactors
STRINGi10090.ENSMUSP00000028234

Structurei

3D structure databases

ProteinModelPortaliP39428
SMRiP39428
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini259 – 405MATHPROSITE-ProRule annotationAdd BLAST147

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili167 – 256By similarityAdd BLAST90

Domaini

The coiled coil domain mediates homo- and hetero-oligomerization.By similarity
The MATH/TRAF domain binds to receptor cytoplasmic domains.
Cleavage by CASP8 liberates a C-terminal fragment that promotes apoptosis and inhibits the activation of NF-kappa-B in response to TNF signaling.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410ISDN Eukaryota
ENOG410YE68 LUCA
GeneTreeiENSGT00550000074359
HOGENOMiHOG000231558
HOVERGENiHBG058222
InParanoidiP39428
KOiK03172
OMAiVCQDPSE
OrthoDBiEOG091G0GHD
TreeFamiTF321154

Family and domain databases

CDDicd03779 MATH_TRAF1, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR002083 MATH/TRAF_dom
IPR012227 TNF_rcpt--assoc_TRAF
IPR008974 TRAF-like
IPR027136 TRAF1
IPR037306 TRAF1_MATH
IPR032070 TRAF_BIRC3-bd
PANTHERiPTHR10131:SF91 PTHR10131:SF91, 1 hit
PfamiView protein in Pfam
PF16673 TRAF_BIRC3_bd, 1 hit
PIRSFiPIRSF015614 TRAF, 1 hit
SMARTiView protein in SMART
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50144 MATH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P39428-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASSSAPDEN EFQFGCPPAP CQDPSEPRVL CCTACLSENL RDDEDRICPK
60 70 80 90 100
CRADNLHPVS PGSPLTQEKV HSDVAEAEIM CPFAGVGCSF KGSPQSMQEH
110 120 130 140 150
EATSQSSHLY LLLAVLKEWK SSPGSNLGSA PMALERNLSE LQLQAAVEAT
160 170 180 190 200
GDLEVDCYRA PCCESQEELA LQHLVKEKLL AQLEEKLRVF ANIVAVLNKE
210 220 230 240 250
VEASHLALAA SIHQSQLDRE HILSLEQRVV ELQQTLAQKD QVLGKLEHSL
260 270 280 290 300
RLMEEASFDG TFLWKITNVT KRCHESVCGR TVSLFSPAFY TAKYGYKLCL
310 320 330 340 350
RLYLNGDGSG KKTHLSLFIV IMRGEYDALL PWPFRNKVTF MLLDQNNREH
360 370 380 390 400
AIDAFRPDLS SASFQRPQSE TNVASGCPLF FPLSKLQSPK HAYVKDDTMF

LKCIVDTSA
Length:409
Mass (Da):45,465
Last modified:July 27, 2011 - v2
Checksum:iADD7F997169D9AAD
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0J9YUH9A0A0J9YUH9_MOUSE
TNF receptor-associated factor 1
Traf1
71Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti222I → L in AAC37663 (PubMed:8069916).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35302 mRNA Translation: AAC37663.1
AK029135 mRNA Translation: BAC26315.1
AK169515 mRNA Translation: BAE41205.1
AK172598 mRNA Translation: BAE43086.1
AL929068 Genomic DNA Translation: CAM27599.1
CH466542 Genomic DNA Translation: EDL08637.1
CCDSiCCDS15956.1
PIRiA54750
RefSeqiNP_001313530.1, NM_001326601.1
NP_033447.2, NM_009421.4
XP_011237352.1, XM_011239050.2
XP_011237353.1, XM_011239051.2
XP_011237354.1, XM_011239052.2
XP_011237355.1, XM_011239053.2
XP_011237356.1, XM_011239054.2
XP_017172618.1, XM_017317129.1
UniGeneiMm.239514

Genome annotation databases

EnsembliENSMUST00000028234; ENSMUSP00000028234; ENSMUSG00000026875
ENSMUST00000113064; ENSMUSP00000108687; ENSMUSG00000026875
ENSMUST00000172159; ENSMUSP00000130759; ENSMUSG00000026875
GeneIDi22029
KEGGimmu:22029
UCSCiuc008jjj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35302 mRNA Translation: AAC37663.1
AK029135 mRNA Translation: BAC26315.1
AK169515 mRNA Translation: BAE41205.1
AK172598 mRNA Translation: BAE43086.1
AL929068 Genomic DNA Translation: CAM27599.1
CH466542 Genomic DNA Translation: EDL08637.1
CCDSiCCDS15956.1
PIRiA54750
RefSeqiNP_001313530.1, NM_001326601.1
NP_033447.2, NM_009421.4
XP_011237352.1, XM_011239050.2
XP_011237353.1, XM_011239051.2
XP_011237354.1, XM_011239052.2
XP_011237355.1, XM_011239053.2
XP_011237356.1, XM_011239054.2
XP_017172618.1, XM_017317129.1
UniGeneiMm.239514

3D structure databases

ProteinModelPortaliP39428
SMRiP39428
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204302, 6 interactors
DIPiDIP-260N
IntActiP39428, 35 interactors
STRINGi10090.ENSMUSP00000028234

Protein family/group databases

MoonDBiP39428 Predicted

PTM databases

iPTMnetiP39428
PhosphoSitePlusiP39428

Proteomic databases

EPDiP39428
MaxQBiP39428
PaxDbiP39428
PeptideAtlasiP39428
PRIDEiP39428

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028234; ENSMUSP00000028234; ENSMUSG00000026875
ENSMUST00000113064; ENSMUSP00000108687; ENSMUSG00000026875
ENSMUST00000172159; ENSMUSP00000130759; ENSMUSG00000026875
GeneIDi22029
KEGGimmu:22029
UCSCiuc008jjj.1 mouse

Organism-specific databases

CTDi7185
MGIiMGI:101836 Traf1

Phylogenomic databases

eggNOGiENOG410ISDN Eukaryota
ENOG410YE68 LUCA
GeneTreeiENSGT00550000074359
HOGENOMiHOG000231558
HOVERGENiHBG058222
InParanoidiP39428
KOiK03172
OMAiVCQDPSE
OrthoDBiEOG091G0GHD
TreeFamiTF321154

Enzyme and pathway databases

ReactomeiR-MMU-5357905 Regulation of TNFR1 signaling
R-MMU-5357956 TNFR1-induced NFkappaB signaling pathway

Miscellaneous databases

PROiPR:P39428
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026875 Expressed in 94 organ(s), highest expression level in subcutaneous adipose tissue
CleanExiMM_TRAF1
ExpressionAtlasiP39428 baseline and differential
GenevisibleiP39428 MM

Family and domain databases

CDDicd03779 MATH_TRAF1, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR002083 MATH/TRAF_dom
IPR012227 TNF_rcpt--assoc_TRAF
IPR008974 TRAF-like
IPR027136 TRAF1
IPR037306 TRAF1_MATH
IPR032070 TRAF_BIRC3-bd
PANTHERiPTHR10131:SF91 PTHR10131:SF91, 1 hit
PfamiView protein in Pfam
PF16673 TRAF_BIRC3_bd, 1 hit
PIRSFiPIRSF015614 TRAF, 1 hit
SMARTiView protein in SMART
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50144 MATH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRAF1_MOUSE
AccessioniPrimary (citable) accession number: P39428
Secondary accession number(s): Q8CE28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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