UniProtKB - P39406 (RSMC_ECOLI)
Protein
Ribosomal RNA small subunit methyltransferase C
Gene
rsmC
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically methylates the guanine in position 1207 of 16S rRNA in the 30S particle.
Catalytic activityi
- guanosine1207 in 16S rRNA + S-adenosyl-L-methionine = H+ + N2-methylguanosine1207 in 16S rRNA + S-adenosyl-L-homocysteineEC:2.1.1.172
Cofactori
GO - Molecular functioni
- 16S rRNA (guanine(1207)-N(2))-methyltransferase activity Source: EcoCyc
- nucleic acid binding Source: InterPro
- rRNA (guanine-N2-)-methyltransferase activity Source: UniProtKB
GO - Biological processi
- rRNA base methylation Source: EcoCyc
- rRNA methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | rRNA processing |
Ligand | Magnesium, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:G7950-MONOMER MetaCyc:G7950-MONOMER |
BRENDAi | 2.1.1.172, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA small subunit methyltransferase C (EC:2.1.1.172)Alternative name(s): 16S rRNA m2G1207 methyltransferase rRNA (guanine-N(2)-)-methyltransferase RsmC |
Gene namesi | Name:rsmC Synonyms:yjjT Ordered Locus Names:b4371, JW4333 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 86 | K → S: Reduces activity by 84%; when associated with S-88. 1 Publication | 1 | |
Mutagenesisi | 88 | K → S: Reduces activity by 84%; when associated with S-86. 1 Publication | 1 | |
Mutagenesisi | 202 | D → A: Abolishes affinity for S-adenosyl-L-methionine. Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 227 | D → A: Strongly reduces affinity for S-adenosyl-L-methionine. Reduces activity by 87%. 1 Publication | 1 | |
Mutagenesisi | 268 | N → A: Reduces affinity for S-adenosyl-L-methionine. Reduces activity by 80%. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000097490 | 2 – 343 | Ribosomal RNA small subunit methyltransferase CAdd BLAST | 342 |
Proteomic databases
jPOSTi | P39406 |
PaxDbi | P39406 |
PRIDEi | P39406 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262778, 51 interactors |
DIPi | DIP-10804N |
IntActi | P39406, 15 interactors |
STRINGi | 511145.b4371 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P39406 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P39406 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2813, Bacteria |
HOGENOMi | CLU_049581_0_1_6 |
InParanoidi | P39406 |
PhylomeDBi | P39406 |
Family and domain databases
HAMAPi | MF_01862, 16SrRNA_methyltr_C, 1 hit |
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR013675, Mtase_sm_N IPR023543, rRNA_ssu_MeTfrase_C IPR029063, SAM-dependent_MTases IPR007848, Small_mtfrase_dom |
Pfami | View protein in Pfam PF05175, MTS, 1 hit PF08468, MTS_N, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P39406-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAFTPASEV LLRHSDDFEQ SRILFAGDLQ DDLPARLDTA ASRAHTQQFH
60 70 80 90 100
HWQVLSRQMG DNARFSLVAT ADDVADCDTL IYYWPKNKPE AQFQLMNLLS
110 120 130 140 150
LLPVGTDIFV VGENRSGVRS AEQMLADYAP LNKVDSARRC GLYFGRLEKQ
160 170 180 190 200
PVFDAEKFWG EYSVDGLTVK TLPGVFSRDG LDVGSQLLLS TLTPHTKGKV
210 220 230 240 250
LDVGCGAGVL SVAFARHSPK IRLTLCDVSA PAVEASRATL AANGVEGEVF
260 270 280 290 300
ASNVFSEVKG RFDMIISNPP FHDGMQTSLD AAQTLIRGAV RHLNSGGELR
310 320 330 340
IVANAFLPYP DVLDETFGFH EVIAQTGRFK VYRAIMTRQA KKG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97267.1 U00096 Genomic DNA Translation: AAC77324.1 AP009048 Genomic DNA Translation: BAE78359.1 |
PIRi | S56595 |
RefSeqi | NP_418788.1, NC_000913.3 WP_001272330.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77324; AAC77324; b4371 BAE78359; BAE78359; BAE78359 |
GeneIDi | 49586223 948892 |
KEGGi | ecj:JW4333 eco:b4371 |
PATRICi | fig|1411691.4.peg.2317 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97267.1 U00096 Genomic DNA Translation: AAC77324.1 AP009048 Genomic DNA Translation: BAE78359.1 |
PIRi | S56595 |
RefSeqi | NP_418788.1, NC_000913.3 WP_001272330.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2PJD | X-ray | 2.10 | A | 1-343 | [»] | |
SMRi | P39406 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262778, 51 interactors |
DIPi | DIP-10804N |
IntActi | P39406, 15 interactors |
STRINGi | 511145.b4371 |
Proteomic databases
jPOSTi | P39406 |
PaxDbi | P39406 |
PRIDEi | P39406 |
Genome annotation databases
EnsemblBacteriai | AAC77324; AAC77324; b4371 BAE78359; BAE78359; BAE78359 |
GeneIDi | 49586223 948892 |
KEGGi | ecj:JW4333 eco:b4371 |
PATRICi | fig|1411691.4.peg.2317 |
Organism-specific databases
EchoBASEi | EB2481 |
Phylogenomic databases
eggNOGi | COG2813, Bacteria |
HOGENOMi | CLU_049581_0_1_6 |
InParanoidi | P39406 |
PhylomeDBi | P39406 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7950-MONOMER MetaCyc:G7950-MONOMER |
BRENDAi | 2.1.1.172, 2026 |
Miscellaneous databases
EvolutionaryTracei | P39406 |
PROi | PR:P39406 |
Family and domain databases
HAMAPi | MF_01862, 16SrRNA_methyltr_C, 1 hit |
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR013675, Mtase_sm_N IPR023543, rRNA_ssu_MeTfrase_C IPR029063, SAM-dependent_MTases IPR007848, Small_mtfrase_dom |
Pfami | View protein in Pfam PF05175, MTS, 1 hit PF08468, MTS_N, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RSMC_ECOLI | |
Accessioni | P39406Primary (citable) accession number: P39406 Secondary accession number(s): Q2M5U7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 156 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families