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Protein

L-galactonate-5-dehydrogenase

Gene

lgoD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of L-galactonate to D-tagaturonate. Required for growth on L-galactonate as the sole carbon source. In vitro, can also use L-gulonate.2 Publications

Catalytic activityi

L-galactonate + NAD+ = D-tagaturonate + NADH.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Activity regulationi

Inhibited by EDTA.1 Publication

Kineticsi

kcat is 0.51 sec(-1) with L-galactonate.1 Publication
  1. KM=19.5 mM for L-galactonate1 Publication
  1. Vmax=0.8 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi40Zinc 1; catalyticBy similarity1
Metal bindingi65Zinc 1; catalyticBy similarity1
Metal bindingi92Zinc 2By similarity1
Metal bindingi95Zinc 2By similarity1
Metal bindingi98Zinc 2By similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi146Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • L-galactonate catabolic process Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:G7945-MONOMER
MetaCyc:G7945-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
L-galactonate-5-dehydrogenase1 Publication (EC:1.1.1.-1 Publication)
Gene namesi
Name:lgoDImported
Synonyms:yjjN
Ordered Locus Names:b4358, JW5793
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12590 lgoD

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to grow on L-galactonate as sole carbon source.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001608951 – 340L-galactonate-5-dehydrogenaseAdd BLAST340

Proteomic databases

PaxDbiP39400
PRIDEiP39400

Expressioni

Inductioni

Highly up-regulated during growth on L-galactonate.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261389, 22 interactors
STRINGi316407.85677098

Structurei

3D structure databases

ProteinModelPortaliP39400
SMRiP39400
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CPQ Bacteria
COG1063 LUCA
HOGENOMiHOG000294690
InParanoidiP39400
PhylomeDBiP39400

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequencei

Sequence statusi: Complete.

P39400-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTMNVLICQ QPKELVWKQR EIPIPGDNEA LIKIKSVGIC GTDIHAWGGN
60 70 80 90 100
QPFFSYPRVL GHEICGEIVG LGKNIADLKN GQQVAVIPYV ACQQCPACKS
110 120 130 140 150
GRTNCCEKIS VIGVHQDGGF SEYLSVPVAN ILPADGIDPQ AAALIEPFAI
160 170 180 190 200
SAHAVRRAAI APGEQVLVVG AGPIGLGAAA IAKADGAQVV VADTSPARRE
210 220 230 240 250
HVATRLELPL LDPSAEDFDA QLRAQFGGSL AQKVIDATGN QHAMNNTVNL
260 270 280 290 300
IRHGGTVVFV GLFKGELQFS DPEFHKKETT MMGSRNATPE DFAKVGRLMA
310 320 330 340
EGKITADMML THRYPFATLA ETYERDVINN RELIKGVITF
Length:340
Mass (Da):36,448
Last modified:November 24, 2009 - v3
Checksum:i15FD289EFFD05567
GO

Sequence cautioni

The sequence AAA97256 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97256.1 Different initiation.
U00096 Genomic DNA Translation: AAC77314.4
AP009048 Genomic DNA Translation: BAE78348.1
PIRiS56585
RefSeqiNP_418778.4, NC_000913.3
WP_000106030.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77314; AAC77314; b4358
BAE78348; BAE78348; BAE78348
GeneIDi948883
KEGGiecj:JW5793
eco:b4358
PATRICifig|1411691.4.peg.2328

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97256.1 Different initiation.
U00096 Genomic DNA Translation: AAC77314.4
AP009048 Genomic DNA Translation: BAE78348.1
PIRiS56585
RefSeqiNP_418778.4, NC_000913.3
WP_000106030.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP39400
SMRiP39400
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261389, 22 interactors
STRINGi316407.85677098

Proteomic databases

PaxDbiP39400
PRIDEiP39400

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77314; AAC77314; b4358
BAE78348; BAE78348; BAE78348
GeneIDi948883
KEGGiecj:JW5793
eco:b4358
PATRICifig|1411691.4.peg.2328

Organism-specific databases

EchoBASEiEB2475
EcoGeneiEG12590 lgoD

Phylogenomic databases

eggNOGiENOG4105CPQ Bacteria
COG1063 LUCA
HOGENOMiHOG000294690
InParanoidiP39400
PhylomeDBiP39400

Enzyme and pathway databases

BioCyciEcoCyc:G7945-MONOMER
MetaCyc:G7945-MONOMER

Miscellaneous databases

PROiPR:P39400

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLGOD_ECOLI
AccessioniPrimary (citable) accession number: P39400
Secondary accession number(s): Q2M5V8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 24, 2009
Last modified: September 12, 2018
This is version 136 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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