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Entry version 130 (13 Nov 2019)
Sequence version 2 (13 Nov 2007)
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Protein

Pyruvate/proton symporter BtsT

Gene

btsT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Transports pyruvate with a high affinity and specificity. The process is driven by the proton motive force (PubMed:29061664). Part of a nutrient-sensing regulatory network composed of the two-component regulatory systems BtsS/BtsR and YpdA/YpdB, and their respective target proteins, BtsT and YhjX (PubMed:24659770).2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Transport is inhibited by the protonophores 2,4-dinitrophenol (DNP) and carbonyl cyanide m-chlorophenyl hydrazone (CCCP), but not by ionophores such as valinomycin, nonactin and nigericin.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=16.5 µM for pyruvate1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Biological processSymport, Transport

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    EcoCyc:G7942-MONOMER
    ECOL316407:JW5791-MONOMER
    MetaCyc:G7942-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Pyruvate/proton symporter BtsTCurated
    Alternative name(s):
    Brenztraubensaure transporter1 Publication
    Pyruvate/H(+) symporter1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:btsT1 Publication
    Synonyms:yjiY
    Ordered Locus Names:b4354, JW5791
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 5Cytoplasmic1 Publication5
    <p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
    Topological domaini27 – 30Periplasmic1 Publication4
    Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
    Topological domaini52 – 88Cytoplasmic1 PublicationAdd BLAST37
    Transmembranei89 – 109HelicalSequence analysisAdd BLAST21
    Topological domaini110 – 119Periplasmic1 Publication10
    Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
    Topological domaini141 – 163Cytoplasmic1 PublicationAdd BLAST23
    Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
    Topological domaini185 – 191Periplasmic1 Publication7
    Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
    Topological domaini213 – 222Cytoplasmic1 Publication10
    Transmembranei223 – 243HelicalSequence analysisAdd BLAST21
    Topological domaini244 – 257Periplasmic1 PublicationAdd BLAST14
    Transmembranei258 – 278HelicalSequence analysisAdd BLAST21
    Topological domaini279 – 282Cytoplasmic1 Publication4
    Transmembranei283 – 303HelicalSequence analysisAdd BLAST21
    Topological domaini304 – 326Periplasmic1 PublicationAdd BLAST23
    Transmembranei327 – 347HelicalSequence analysisAdd BLAST21
    Topological domaini348 – 374Cytoplasmic1 PublicationAdd BLAST27
    Transmembranei375 – 395HelicalSequence analysisAdd BLAST21
    Topological domaini396 – 484Periplasmic1 PublicationAdd BLAST89
    Transmembranei485 – 505HelicalSequence analysisAdd BLAST21
    Topological domaini506 – 531Cytoplasmic1 PublicationAdd BLAST26
    Transmembranei532 – 552HelicalSequence analysisAdd BLAST21
    Topological domaini553 – 568Periplasmic1 PublicationAdd BLAST16
    Transmembranei569 – 589HelicalSequence analysisAdd BLAST21
    Topological domaini590 – 596Cytoplasmic1 Publication7
    Transmembranei597 – 617HelicalSequence analysisAdd BLAST21
    Topological domaini618 – 668Periplasmic1 PublicationAdd BLAST51
    Transmembranei669 – 689HelicalSequence analysisAdd BLAST21
    Topological domaini690 – 716Cytoplasmic1 PublicationAdd BLAST27

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001900501 – 716Pyruvate/proton symporter BtsTAdd BLAST716

    Proteomic databases

    jPOST - Japan Proteome Standard Repository/Database

    More...
    jPOSTi
    P39396

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P39396

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P39396

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    Strongly induced at the onset of the stationary-growth phase. Expression is dependent on the BtsS/BtsR two-component regulatory system, and on cAMP and the cAMP receptor protein (CRP). Repressed by LeuO. Monocistronic operon. Regulated at post-transcriptional level by CsrA (PubMed:24659770).3 Publications

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Interacts with BtsS and YpdA.

    1 Publication

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGrid)

    More...
    BioGridi
    4261745, 187 interactors

    Database of interacting proteins

    More...
    DIPi
    DIP-12649N

    Protein interaction database and analysis system

    More...
    IntActi
    P39396, 2 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    511145.b4354

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105D22 Bacteria
    COG1966 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000220271

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P39396

    KEGG Orthology (KO)

    More...
    KOi
    K23676

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P39396

    Family and domain databases

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR025299 CstA_C
    IPR003706 CstA_N

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF02554 CstA, 1 hit
    PF13722 CstA_5TM, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P39396-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MDTKKIFKHI PWVILGIIGA FCLAVVALRR GEHISALWIV VASVSVYLVA
    60 70 80 90 100
    YRYYSLYIAQ KVMKLDPTRA TPAVINNDGL NYVPTNRYVL FGHHFAAIAG
    110 120 130 140 150
    AGPLVGPVLA AQMGYLPGTL WLLAGVVLAG AVQDFMVLFI SSRRNGASLG
    160 170 180 190 200
    EMIKEEMGPV PGTIALFGCF LIMIIILAVL ALIVVKALAE SPWGVFTVCS
    210 220 230 240 250
    TVPIALFMGI YMRFIRPGRV GEVSVIGIVL LVASIYFGGV IAHDPYWGPA
    260 270 280 290 300
    LTFKDTTITF ALIGYAFVSA LLPVWLILAP RDYLATFLKI GVIVGLALGI
    310 320 330 340 350
    VVLNPELKMP AMTQYIDGTG PLWKGALFPF LFITIACGAV SGFHALISSG
    360 370 380 390 400
    TTPKLLANET DARFIGYGAM LMESFVAIMA LVAASIIEPG LYFAMNTPPA
    410 420 430 440 450
    GLGITMPNLH EMGGENAPII MAQLKDVTAH AAATVSSWGF VISPEQILQT
    460 470 480 490 500
    AKDIGEPSVL NRAGGAPTLA VGIAHVFHKV LPMADMGFWY HFGILFEALF
    510 520 530 540 550
    ILTALDAGTR SGRFMLQDLL GNFIPFLKKT DSLVAGIIGT AGCVGLWGYL
    560 570 580 590 600
    LYQGVVDPLG GVKSLWPLFG ISNQMLAAVA LVLGTVVLIK MKRTQYIWVT
    610 620 630 640 650
    VVPAVWLLIC TTWALGLKLF STNPQMEGFF YMASQYKEKI ANGTDLTAQQ
    660 670 680 690 700
    IANMNHIVVN NYTNAGLSIL FLIVVYSIIF YGFKTWLAVR NSDKRTDKET
    710
    PYVPIPEGGV KISSHH
    Length:716
    Mass (Da):77,324
    Last modified:November 13, 2007 - v2
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i664E7B6169F15B4D
    GO

    <p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

    The sequence AAA97251 differs from that shown. Reason: Erroneous initiation.Curated

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    U14003 Genomic DNA Translation: AAA97251.1 Different initiation.
    U00096 Genomic DNA Translation: AAC77310.2
    AP009048 Genomic DNA Translation: BAE78344.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    S56580

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_418774.2, NC_000913.3
    WP_001299714.1, NZ_LN832404.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAC77310; AAC77310; b4354
    BAE78344; BAE78344; BAE78344

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    948914

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ecj:JW5791
    eco:b4354

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|1411691.4.peg.2332

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U14003 Genomic DNA Translation: AAA97251.1 Different initiation.
    U00096 Genomic DNA Translation: AAC77310.2
    AP009048 Genomic DNA Translation: BAE78344.1
    PIRiS56580
    RefSeqiNP_418774.2, NC_000913.3
    WP_001299714.1, NZ_LN832404.1

    3D structure databases

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    SWISS-MODEL Interactive Workspace

    More...
    SWISS-MODEL-Workspacei
    Submit a new modelling project...

    Protein-protein interaction databases

    BioGridi4261745, 187 interactors
    DIPiDIP-12649N
    IntActiP39396, 2 interactors
    STRINGi511145.b4354

    Proteomic databases

    jPOSTiP39396
    PaxDbiP39396
    PRIDEiP39396

    Genome annotation databases

    EnsemblBacteriaiAAC77310; AAC77310; b4354
    BAE78344; BAE78344; BAE78344
    GeneIDi948914
    KEGGiecj:JW5791
    eco:b4354
    PATRICifig|1411691.4.peg.2332

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...
    EchoBASEi
    EB2472

    Phylogenomic databases

    eggNOGiENOG4105D22 Bacteria
    COG1966 LUCA
    HOGENOMiHOG000220271
    InParanoidiP39396
    KOiK23676
    PhylomeDBiP39396

    Enzyme and pathway databases

    BioCyciEcoCyc:G7942-MONOMER
    ECOL316407:JW5791-MONOMER
    MetaCyc:G7942-MONOMER

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:P39396

    Family and domain databases

    InterProiView protein in InterPro
    IPR025299 CstA_C
    IPR003706 CstA_N
    PfamiView protein in Pfam
    PF02554 CstA, 1 hit
    PF13722 CstA_5TM, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBTST_ECOLI
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P39396
    Secondary accession number(s): Q2M5W2
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 13, 2007
    Last modified: November 13, 2019
    This is version 130 of the entry and version 2 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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