UniProtKB - P39358 (YJHG_ECOLI)
Protein
D-xylonate dehydratase YjhG
Gene
yjhG
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the dehydration of D-xylonic acid to form 2-dehydro-3-deoxy-D-pentonate.1 Publication1 Publication
Catalytic activityi
- EC:4.2.1.821 Publication1 Publication
Activity regulationi
Activity is increased in the presence of Mn+ and Mg2+. Inhibited by thiol compounds.1 Publication
Kineticsi
kcat is 0.33 min(-1).1 Publication
- KM=4.88 mM for D-xylonate1 Publication
pH dependencei
Optimum pH is 8.0.1 Publication
Temperature dependencei
Optimum temperature is 30 degrees Celsius.1 Publication
GO - Molecular functioni
- hydro-lyase activity Source: GO_Central
- xylonate dehydratase activity Source: EcoCyc
GO - Biological processi
- aldonic acid catabolic process Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Enzyme and pathway databases
BioCyci | EcoCyc:G7910-MONOMER MetaCyc:G7910-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:yjhG Ordered Locus Names:b4297, JW4259 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Disruption mutant has reduced ability to catabolize D-xylonic acid. YjhG-yagF double mutant cannot use D-xylonate as the sole source of carbon.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000103561 | 1 – 655 | D-xylonate dehydratase YjhGAdd BLAST | 655 |
Proteomic databases
jPOSTi | P39358 |
PaxDbi | P39358 |
PRIDEi | P39358 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260973, 10 interactors 851170, 1 interactor |
IntActi | P39358, 4 interactors |
STRINGi | 511145.b4297 |
Family & Domainsi
Sequence similaritiesi
Belongs to the IlvD/Edd family.Curated
Phylogenomic databases
eggNOGi | COG0129, Bacteria |
HOGENOMi | CLU_014271_5_0_6 |
PhylomeDBi | P39358 |
Family and domain databases
Gene3Di | 3.50.30.80, 1 hit |
InterProi | View protein in InterPro IPR017798, Dehydratase_YjhG/YagF IPR042096, Dihydro-acid_dehy_C IPR000581, DiOHA_6PGluconate_deHydtase IPR020558, DiOHA_6PGluconate_deHydtase_CS IPR037237, IlvD/EDD_N |
Pfami | View protein in Pfam PF00920, ILVD_EDD, 1 hit |
SUPFAMi | SSF143975, SSF143975, 1 hit |
TIGRFAMsi | TIGR03432, yjhG_yagF, 1 hit |
PROSITEi | View protein in PROSITE PS00886, ILVD_EDD_1, 1 hit PS00887, ILVD_EDD_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P39358-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVRNIFADE SHDIYTVRTH ADGPDGELPL TAEMLINRPS GDLFGMTMNA
60 70 80 90 100
GMGWSPDELD RDGILLLSTL GGLRGADGKP VALALHQGHY ELDIQMKAAA
110 120 130 140 150
EVIKANHALP YAVYVSDPCD GRTQGTTGMF DSLPYRNDAS MVMRRLIRSL
160 170 180 190 200
PDAKAVIGVA SCDKGLPATM MALAAQHNIA TVLVPGGATL PAKDGEDNGK
210 220 230 240 250
VQTIGARFAN GELSLQDARR AGCKACASSG GGCQFLGTAG TSQVVAEGLG
260 270 280 290 300
LAIPHSALAP SGEPVWREIA RASARAALNL SQKGITTREI LTDKAIENAM
310 320 330 340 350
TVHAAFGGST NLLLHIPAIA HQAGCHIPTV DDWIRINKRV PRLVSVLPNG
360 370 380 390 400
PVYHPTVNAF MAGGVPEVML HLRSLGLLHE DVMTVTGSTL KENLDWWEHS
410 420 430 440 450
ERRQRFKQLL LDQEQINADE VIMSPQQAKA RGLTSTITFP VGNIAPEGSV
460 470 480 490 500
IKSTAIDPSM IDEQGIYYHK GVAKVYLSEK SAIYDIKHDK IKAGDILVII
510 520 530 540 550
GVGPSGTGME ETYQVTSALK HLSYGKHVSL ITDARFSGVS TGACIGHVGP
560 570 580 590 600
EALAGGPIGK LRTGDLIEIK IDCRELHGEV NFLGTRSDEQ LPSQEEATAI
610 620 630 640 650
LNARPSHQDL LPDPELPDDT RLWAMLQAVS GGTWTGCIYD VNKIGAALRD
FMNKN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 555 – 556 | GG → EA in AAA97193 (PubMed:7610040).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97193.1 U00096 Genomic DNA Translation: AAC77253.1 AP009048 Genomic DNA Translation: BAE78288.1 |
PIRi | C65243 |
RefSeqi | NP_418717.1, NC_000913.3 WP_000116326.1, NZ_SSUV01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC77253; AAC77253; b4297 BAE78288; BAE78288; BAE78288 |
GeneIDi | 57730636 946829 |
KEGGi | ecj:JW4259 eco:b4297 |
PATRICi | fig|1411691.4.peg.2400 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97193.1 U00096 Genomic DNA Translation: AAC77253.1 AP009048 Genomic DNA Translation: BAE78288.1 |
PIRi | C65243 |
RefSeqi | NP_418717.1, NC_000913.3 WP_000116326.1, NZ_SSUV01000012.1 |
3D structure databases
SMRi | P39358 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260973, 10 interactors 851170, 1 interactor |
IntActi | P39358, 4 interactors |
STRINGi | 511145.b4297 |
Proteomic databases
jPOSTi | P39358 |
PaxDbi | P39358 |
PRIDEi | P39358 |
Genome annotation databases
EnsemblBacteriai | AAC77253; AAC77253; b4297 BAE78288; BAE78288; BAE78288 |
GeneIDi | 57730636 946829 |
KEGGi | ecj:JW4259 eco:b4297 |
PATRICi | fig|1411691.4.peg.2400 |
Organism-specific databases
EchoBASEi | EB2437 |
Phylogenomic databases
eggNOGi | COG0129, Bacteria |
HOGENOMi | CLU_014271_5_0_6 |
PhylomeDBi | P39358 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7910-MONOMER MetaCyc:G7910-MONOMER |
Miscellaneous databases
PROi | PR:P39358 |
Family and domain databases
Gene3Di | 3.50.30.80, 1 hit |
InterProi | View protein in InterPro IPR017798, Dehydratase_YjhG/YagF IPR042096, Dihydro-acid_dehy_C IPR000581, DiOHA_6PGluconate_deHydtase IPR020558, DiOHA_6PGluconate_deHydtase_CS IPR037237, IlvD/EDD_N |
Pfami | View protein in Pfam PF00920, ILVD_EDD, 1 hit |
SUPFAMi | SSF143975, SSF143975, 1 hit |
TIGRFAMsi | TIGR03432, yjhG_yagF, 1 hit |
PROSITEi | View protein in PROSITE PS00886, ILVD_EDD_1, 1 hit PS00887, ILVD_EDD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YJHG_ECOLI | |
Accessioni | P39358Primary (citable) accession number: P39358 Secondary accession number(s): Q2M618 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | July 15, 1998 | |
Last modified: | February 10, 2021 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families