UniProtKB - P39333 (BDCA_ECOLI)
Protein
Cyclic-di-GMP-binding biofilm dispersal mediator protein
Gene
bdcA
Organism
Escherichia coli (strain K12)
Status
Functioni
Increases biofilm dispersal. Acts by binding directly to the signaling molecule cyclic-di-GMP, which decreases the intracellular concentration of cyclic-di-GMP and leads to biofilm dispersal. Also controls other biofilm-related phenotypes such as cell motility, cell size, cell aggregation and production of extracellular DNA and extracellular polysaccharides (EPS). Does not act as a phosphodiesterase.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 132 | SubstrateBy similarity | 1 | |
Active sitei | 146 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 34 | NAD or NADPBy similarityAdd BLAST | 25 |
GO - Molecular functioni
- cyclic-di-GMP binding Source: EcoCyc
- NADPH binding Source: EcoCyc
- oxidoreductase activity Source: InterPro
GO - Biological processi
- single-species biofilm formation Source: EcoCyc
Keywordsi
Ligand | c-di-GMP |
Enzyme and pathway databases
BioCyci | EcoCyc:G7880-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclic-di-GMP-binding biofilm dispersal mediator protein |
Gene namesi | Name:bdcA Synonyms:yjgI Ordered Locus Names:b4249, JW4207 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutant shows decreased biofilm dispersal. Deletion increases the cyclic-di GMP concentration in the cell.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 50 | E → Q or V: Shows higher affinity for cyclic-di-GMP, increases swimming motility and biofilm dispersal. Biofilm formation is almost completely removed. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054839 | 1 – 237 | Cyclic-di-GMP-binding biofilm dispersal mediator proteinAdd BLAST | 237 |
Proteomic databases
PaxDbi | P39333 |
PRIDEi | P39333 |
Expressioni
Inductioni
Expression is time dependent in biofilms and is controlled by BdcR.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259560, 12 interactors |
DIPi | DIP-12604N |
IntActi | P39333, 2 interactors |
STRINGi | 511145.b4249 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P39333 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | COG1028, Bacteria |
HOGENOMi | CLU_010194_1_3_6 |
InParanoidi | P39333 |
PhylomeDBi | P39333 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Complete.
P39333-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGAFTGKTVL ILGGSRGIGA AIVRRFVTDG ANVRFTYAGS KDAAKRLAQE
60 70 80 90 100
TGATAVFTDS ADRDAVIDVV RKSGALDILV VNAGIGVFGE ALELNADDID
110 120 130 140 150
RLFKINIHAP YHASVEAARQ MPEGGRILII GSVNGDRMPV AGMAAYAASK
160 170 180 190 200
SALQGMARGL ARDFGPRGIT INVVQPGPID TDANPANGPM RDMLHSLMAI
210 220 230
KRHGQPEEVA GMVAWLAGPE ASFVTGAMHT IDGAFGA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97146.1 Sequence problems. U00096 Genomic DNA Translation: AAC77206.1 AP009048 Genomic DNA Translation: BAE78247.1 |
PIRi | D65237 |
RefSeqi | NP_418670.1, NC_000913.3 WP_000500727.1, NZ_STEB01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC77206; AAC77206; b4249 BAE78247; BAE78247; BAE78247 |
GeneIDi | 57730700 948765 |
KEGGi | ecj:JW4207 eco:b4249 |
PATRICi | fig|511145.12.peg.4380 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97146.1 Sequence problems. U00096 Genomic DNA Translation: AAC77206.1 AP009048 Genomic DNA Translation: BAE78247.1 |
PIRi | D65237 |
RefSeqi | NP_418670.1, NC_000913.3 WP_000500727.1, NZ_STEB01000013.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5Z2L | X-ray | 1.70 | A/B/C/D/E/F/G/H/I/J/K/L | 1-237 | [»] | |
SMRi | P39333 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259560, 12 interactors |
DIPi | DIP-12604N |
IntActi | P39333, 2 interactors |
STRINGi | 511145.b4249 |
Proteomic databases
PaxDbi | P39333 |
PRIDEi | P39333 |
Genome annotation databases
EnsemblBacteriai | AAC77206; AAC77206; b4249 BAE78247; BAE78247; BAE78247 |
GeneIDi | 57730700 948765 |
KEGGi | ecj:JW4207 eco:b4249 |
PATRICi | fig|511145.12.peg.4380 |
Organism-specific databases
EchoBASEi | EB2418 |
Phylogenomic databases
eggNOGi | COG1028, Bacteria |
HOGENOMi | CLU_010194_1_3_6 |
InParanoidi | P39333 |
PhylomeDBi | P39333 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7880-MONOMER |
Miscellaneous databases
PROi | PR:P39333 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BDCA_ECOLI | |
Accessioni | P39333Primary (citable) accession number: P39333 Secondary accession number(s): Q2M659 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | July 15, 1998 | |
Last modified: | April 7, 2021 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families