UniProtKB - P39301 (ULAA_ECOLI)
Protein
Ascorbate-specific PTS system EIIC component
Gene
ulaA
Organism
Escherichia coli (strain K12)
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II UlaABC PTS system is involved in ascorbate transport.1 Publication1 Publication
Kineticsi
- KM=18 µM for L-ascorbate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 314 | Ascorbate1 Publication | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein-phosphocysteine-L-ascorbate-phosphotransferase system transporter activity Source: EcoCyc
GO - Biological processi
- L-ascorbic acid transmembrane transport Source: EcoCyc
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Keywordsi
Biological process | Phosphotransferase system, Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:SGAT-MONOMER MetaCyc:SGAT-MONOMER |
Protein family/group databases
TCDBi | 4.A.7.1.1, the pts l-ascorbate (l-asc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Ascorbate-specific PTS system EIIC component1 PublicationAlternative name(s): Ascorbate-specific permease IIC component UlaA1 Publication |
Gene namesi | Name:ulaA1 Publication Synonyms:sgaT, yjfS Ordered Locus Names:b4193, JW5744 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 14 – 34 | Helical1 PublicationAdd BLAST | 21 | |
Transmembranei | 38 – 58 | Helical1 PublicationAdd BLAST | 21 | |
Transmembranei | 101 – 121 | Helical1 PublicationAdd BLAST | 21 | |
Transmembranei | 141 – 161 | Helical1 PublicationAdd BLAST | 21 | |
Transmembranei | 233 – 253 | Helical1 PublicationAdd BLAST | 21 | |
Transmembranei | 263 – 283 | Helical1 PublicationAdd BLAST | 21 | |
Transmembranei | 316 – 336 | Helical1 PublicationAdd BLAST | 21 | |
Transmembranei | 338 – 358 | Helical1 PublicationAdd BLAST | 21 | |
Transmembranei | 379 – 399 | Helical1 PublicationAdd BLAST | 21 | |
Transmembranei | 427 – 447 | Helical1 PublicationAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene are unable to use L-ascorbate.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097714 | 1 – 465 | Ascorbate-specific PTS system EIIC componentAdd BLAST | 465 |
Proteomic databases
PaxDbi | P39301 |
PRIDEi | P39301 |
Expressioni
Inductioni
Induced by L-ascorbate. Repressed by UlaR.3 Publications
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
P39301
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-556234,EBI-556234 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262713, 11 interactors |
DIPi | DIP-10870N |
IntActi | P39301, 1 interactor |
STRINGi | 511145.b4193 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P39301 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 86 – 87 | Ascorbate binding1 Publication | 2 | |
Regioni | 135 – 139 | Ascorbate binding1 Publication | 5 | |
Regioni | 194 – 195 | Ascorbate binding1 Publication | 2 |
Domaini
In classical PTS systems, the PTS EIIC type-2 domain forms the translocation channel and contains the specific substrate-binding site. UlaA does not exhibit the topological features of any recognized enzyme IIC.Curated
Sequence similaritiesi
Belongs to the UlaA family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3037, Bacteria |
HOGENOMi | CLU_031784_1_0_6 |
InParanoidi | P39301 |
PhylomeDBi | P39301 |
Family and domain databases
InterProi | View protein in InterPro IPR004703, PTS_sugar-sp_permease |
Pfami | View protein in Pfam PF03611, EIIC-GAT, 1 hit |
i Sequence
Sequence statusi: Complete.
P39301-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEILYNIFTV FFNQVMTNAP LLLGIVTCLG YILLRKSVSV IIKGTIKTII
60 70 80 90 100
GFMLLQAGSG ILTSTFKPVV AKMSEVYGIN GAISDTYASM MATIDRMGDA
110 120 130 140 150
YSWVGYAVLL ALALNICYVL LRRITGIRTI MLTGHIMFQQ AGLIAVTLFI
160 170 180 190 200
FGYSMWTTII CTAILVSLYW GITSNMMYKP TQEVTDGCGF SIGHQQQFAS
210 220 230 240 250
WIAYKVAPFL GKKEESVEDL KLPGWLNIFH DNIVSTAIVM TIFFGAILLS
260 270 280 290 300
FGIDTVQAMA GKVHWTVYIL QTGFSFAVAI FIITQGVRMF VAELSEAFNG
310 320 330 340 350
ISQRLIPGAV LAIDCAAIYS FAPNAVVWGF MWGTIGQLIA VGILVACGSS
360 370 380 390 400
ILIIPGFIPM FFSNATIGVF ANHFGGWRAA LKICLVMGMI EIFGCVWAVK
410 420 430 440 450
LTGMSAWMGM ADWSILAPPM MQGFFSIGIA FMAVIIVIAL AYMFFAGRAL
460
RAEEDAEKQL AEQSA
Sequence cautioni
The sequence AAA97089 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 451 – 465 | RAEED…AEQSA → AQKKMQKNNWQNSLLNKEF in AAA97089 (PubMed:7610040).CuratedAdd BLAST | 15 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97089.1 Different initiation. U00096 Genomic DNA Translation: AAC77150.2 AP009048 Genomic DNA Translation: BAE78194.1 |
PIRi | D65230 |
RefSeqi | NP_418614.4, NC_000913.3 WP_001350568.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77150; AAC77150; b4193 BAE78194; BAE78194; BAE78194 |
GeneIDi | 948717 |
KEGGi | ecj:JW5744 eco:b4193 |
PATRICi | fig|1411691.4.peg.2508 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97089.1 Different initiation. U00096 Genomic DNA Translation: AAC77150.2 AP009048 Genomic DNA Translation: BAE78194.1 |
PIRi | D65230 |
RefSeqi | NP_418614.4, NC_000913.3 WP_001350568.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4RP8 | X-ray | 2.36 | A/C | 1-465 | [»] | |
4RP9 | X-ray | 1.65 | A | 1-465 | [»] | |
SMRi | P39301 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262713, 11 interactors |
DIPi | DIP-10870N |
IntActi | P39301, 1 interactor |
STRINGi | 511145.b4193 |
Protein family/group databases
TCDBi | 4.A.7.1.1, the pts l-ascorbate (l-asc) family |
Proteomic databases
PaxDbi | P39301 |
PRIDEi | P39301 |
Genome annotation databases
EnsemblBacteriai | AAC77150; AAC77150; b4193 BAE78194; BAE78194; BAE78194 |
GeneIDi | 948717 |
KEGGi | ecj:JW5744 eco:b4193 |
PATRICi | fig|1411691.4.peg.2508 |
Organism-specific databases
EchoBASEi | EB2386 |
Phylogenomic databases
eggNOGi | COG3037, Bacteria |
HOGENOMi | CLU_031784_1_0_6 |
InParanoidi | P39301 |
PhylomeDBi | P39301 |
Enzyme and pathway databases
BioCyci | EcoCyc:SGAT-MONOMER MetaCyc:SGAT-MONOMER |
Miscellaneous databases
PROi | PR:P39301 |
Family and domain databases
InterProi | View protein in InterPro IPR004703, PTS_sugar-sp_permease |
Pfami | View protein in Pfam PF03611, EIIC-GAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ULAA_ECOLI | |
Accessioni | P39301Primary (citable) accession number: P39301 Secondary accession number(s): Q2M6B2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | April 4, 2006 | |
Last modified: | December 2, 2020 | |
This is version 154 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families