UniProtKB - P39288 (QUEG_ECOLI)
Protein
Epoxyqueuosine reductase
Gene
queG
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).UniRule annotation
Catalytic activityi
- EC:1.17.99.6UniRule annotation
: tRNA-queuosine biosynthesis Pathwayi
This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 193 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 196 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 199 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 203 | Iron-sulfur 2UniRule annotation | 1 | |
Metal bindingi | 246 | Iron-sulfur 2UniRule annotation | 1 | |
Metal bindingi | 249 | Iron-sulfur 2UniRule annotation | 1 | |
Metal bindingi | 253 | Iron-sulfur 1 (4Fe-4S)UniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- epoxyqueuosine reductase activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- queuosine biosynthetic process Source: EcoCyc
- tRNA processing Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Queuosine biosynthesis, tRNA processing |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7843-MONOMER MetaCyc:G7843-MONOMER |
UniPathwayi | UPA00392 |
Names & Taxonomyi
Protein namesi | Recommended name: Epoxyqueuosine reductaseUniRule annotation (EC:1.17.99.6UniRule annotation)Alternative name(s): Queuosine biosynthesis protein QueGUniRule annotation |
Gene namesi | Name:queGUniRule annotation Synonyms:yjeS Ordered Locus Names:b4166, JW4124 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
RNA from mutants accumulates epoxyqueuosine and lacks queuosine.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159304 | 1 – 379 | Epoxyqueuosine reductaseAdd BLAST | 379 |
Proteomic databases
PaxDbi | P39288 |
PRIDEi | P39288 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262703, 4 interactors |
IntActi | P39288, 2 interactors |
STRINGi | 511145.b4166 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 181 – 213 | 4Fe-4S ferredoxin-typeUniRule annotationAdd BLAST | 33 |
Sequence similaritiesi
Belongs to the QueG family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1600, Bacteria |
HOGENOMi | CLU_030790_0_1_6 |
InParanoidi | P39288 |
PhylomeDBi | P39288 |
Family and domain databases
HAMAPi | MF_00916, QueG, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR013542, DUF1730 IPR009051, Helical_ferredxn IPR004453, QueG |
PANTHERi | PTHR30002, PTHR30002, 1 hit |
Pfami | View protein in Pfam PF08331, DUF1730, 1 hit |
SUPFAMi | SSF46548, SSF46548, 1 hit |
TIGRFAMsi | TIGR00276, TIGR00276, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P39288-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEPLDLNQL AQKIKQWGLE LGFQQVGITD TDLSESEPKL QAWLDKQYHG
60 70 80 90 100
EMDWMARHGM LRARPHELLP GTLRVISVRM NYLPANAAFA STLKNPKLGY
110 120 130 140 150
VSRYALGRDY HKLLRNRLKK LGEMIQQHCV SLNFRPFVDS APILERPLAE
160 170 180 190 200
KAGLGWTGKH SLILNREAGS FFFLGELLVD IPLPVDQPVE EGCGKCVACM
210 220 230 240 250
TICPTGAIVE PYTVDARRCI SYLTIELEGA IPEELRPLMG NRIYGCDDCQ
260 270 280 290 300
LICPWNRYSQ LTTEEDFSPR KPLHAPELIE LFAWSEEKFL KVTEGSAIRR
310 320 330 340 350
IGHLRWLRNI AVALGNAPWD ETILTALESR KGEHPLLDEH IAWAIAQQIE
360 370
RRNACIVEVQ LPKKQRLVRV IEKGLPRDA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97062.1 U00096 Genomic DNA Translation: AAC77123.1 AP009048 Genomic DNA Translation: BAE78167.1 |
PIRi | S56391 |
RefSeqi | NP_418587.1, NC_000913.3 WP_001294219.1, NZ_STEB01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC77123; AAC77123; b4166 BAE78167; BAE78167; BAE78167 |
GeneIDi | 57730061 948686 |
KEGGi | ecj:JW4124 eco:b4166 |
PATRICi | fig|1411691.4.peg.2535 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97062.1 U00096 Genomic DNA Translation: AAC77123.1 AP009048 Genomic DNA Translation: BAE78167.1 |
PIRi | S56391 |
RefSeqi | NP_418587.1, NC_000913.3 WP_001294219.1, NZ_STEB01000013.1 |
3D structure databases
SMRi | P39288 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262703, 4 interactors |
IntActi | P39288, 2 interactors |
STRINGi | 511145.b4166 |
Proteomic databases
PaxDbi | P39288 |
PRIDEi | P39288 |
Genome annotation databases
EnsemblBacteriai | AAC77123; AAC77123; b4166 BAE78167; BAE78167; BAE78167 |
GeneIDi | 57730061 948686 |
KEGGi | ecj:JW4124 eco:b4166 |
PATRICi | fig|1411691.4.peg.2535 |
Organism-specific databases
EchoBASEi | EB2374 |
Phylogenomic databases
eggNOGi | COG1600, Bacteria |
HOGENOMi | CLU_030790_0_1_6 |
InParanoidi | P39288 |
PhylomeDBi | P39288 |
Enzyme and pathway databases
UniPathwayi | UPA00392 |
BioCyci | EcoCyc:G7843-MONOMER MetaCyc:G7843-MONOMER |
Miscellaneous databases
PROi | PR:P39288 |
Family and domain databases
HAMAPi | MF_00916, QueG, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR013542, DUF1730 IPR009051, Helical_ferredxn IPR004453, QueG |
PANTHERi | PTHR30002, PTHR30002, 1 hit |
Pfami | View protein in Pfam PF08331, DUF1730, 1 hit |
SUPFAMi | SSF46548, SSF46548, 1 hit |
TIGRFAMsi | TIGR00276, TIGR00276, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 1 hit PS51379, 4FE4S_FER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | QUEG_ECOLI | |
Accessioni | P39288Primary (citable) accession number: P39288 Secondary accession number(s): Q2M6D9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families