UniProtKB - P39286 (RSGA_ECOLI)
Small ribosomal subunit biogenesis GTPase RsgA
rsgA
Functioni
Caution
Cofactori
Kineticsi
- KM=0.41 mM for GTP1 Publication
- KM=1.0 mM for dATP1 Publication
- KM=1.2 mM for ATP1 Publication
- KM=1.3 mM for ITP1 Publication
- KM=1.6 mM for dGTP1 Publication
- KM=2.4 mM for CTP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 297 | ZincUniRule annotation | 1 | |
Metal bindingi | 302 | ZincUniRule annotation | 1 | |
Metal bindingi | 304 | Zinc; via pros nitrogenUniRule annotation | 1 | |
Metal bindingi | 310 | ZincUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 160 – 163 | GTPUniRule annotation | 4 | |
Nucleotide bindingi | 214 – 222 | GTPUniRule annotation | 9 |
GO - Molecular functioni
- GDP binding Source: EcoCyc
- GTPase activity Source: EcoCyc
- GTP binding Source: UniProtKB-UniRule
- guanosine tetraphosphate binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- rRNA binding Source: UniProtKB-KW
GO - Biological processi
- ribosomal small subunit biogenesis Source: EcoCyc
Keywordsi
Molecular function | Hydrolase, RNA-binding, rRNA-binding |
Biological process | Ribosome biogenesis |
Ligand | GTP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:G7841-MONOMER MetaCyc:G7841-MONOMER |
SABIO-RKi | P39286 |
Names & Taxonomyi
Protein namesi | Recommended name: Small ribosomal subunit biogenesis GTPase RsgAUniRule annotation (EC:3.6.1.-UniRule annotation) |
Gene namesi | Name:rsgA1 PublicationUniRule annotation Synonyms:engC, yjeQ Ordered Locus Names:b4161, JW4122 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1 – 20 | Missing : Loss of GMP-PNP-dependent association with 30S ribosomal subunit, increased association with 50S and 70S ribosomes. 1 PublicationAdd BLAST | 20 | |
Mutagenesisi | 220 | K → A: Reduction in GTPase activity, 38% of wild-type kcat for GTP. Strong reduction, 5.2% of wild-type kcat for GTP; when associated with A-221. 1 Publication | 1 | |
Mutagenesisi | 221 | S → A: Reduction in GTPase activity, 22% of wild-type kcat for GTP. GTPase activity not stimulated by 30S ribosomes (PubMed:14973029). Strong reduction, 5.2% of wild-type kcat for GTP; when associated with A-220. 2 Publications | 1 | |
Mutagenesisi | 250 | T → A: Loss of GTPase activity, does not dissociate RbfA. 1 Publication | 1 | |
Mutagenesisi | 298 – 300 | KYR → AYA: About 2-fold decreased binding to mature and immature 30S ribosomes, GTPase activity stimulated by ribosomes. 1 Publication | 3 | |
Mutagenesisi | 320 – 350 | Missing : Slightly increased specific binding to mature and immature 30S ribosomes, GTPase activity not stimulated by ribosomes. No longer removes RbfA from mature 30S ribosomes, increases RbfA-binding about 5-fold. Does not complement a deletion mutant in vivo, fewer 70S ribosomes, slower growth than deletion mutant. 1 PublicationAdd BLAST | 31 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000008149 | 1 – 350 | Small ribosomal subunit biogenesis GTPase RsgAAdd BLAST | 350 |
Proteomic databases
jPOSTi | P39286 |
PaxDbi | P39286 |
PRIDEi | P39286 |
Interactioni
Subunit structurei
Monomer (Probable). All of the protein is associated with ribosomes; the ratio is substoichiometric at 1:200 RsgA/ribosome (PubMed:14973029). Association is tightest with the 30S subunit in the presence of the non-hydrolyzable GTP analog GMP-PNP (PubMed:14973029, PubMed:15466596). 2 cryoelectron microscopy (cryo-EM) structures in complex with 30S ribosomes have been resolved; the protein is determined to bind to different but partially overlapping regions of the 30S ribosomal subunit (PubMed:21788480, PubMed:21960487). One cryo-EM study suggests it contacts ribosomal proteins S3, S12 and S13 as well as 16S rRNA (PubMed:21788480). Another cryo-EM study shows it to bind 16S rRNA, no ribosomal proteins, and to cover the sites of intersubunit bridges B2a, B3 and B7a (PubMed:21960487). Has a significant preference for mature versus immature 30S ribosomes in the presence of GMP-PNP (PubMed:21102555, PubMed:27382067). Another study shows it binds equally well to mature and immature 30S ribosomal subunits in the presence of GMP-PNP (PubMed:25904134).
1 Publication7 PublicationsProtein-protein interaction databases
BioGRIDi | 4261080, 609 interactors |
DIPi | DIP-12581N |
IntActi | P39286, 33 interactors |
MINTi | P39286 |
STRINGi | 511145.b4161 |
Structurei
3D structure databases
SMRi | P39286 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P39286 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 104 – 273 | CP-type GPROSITE-ProRule annotationAdd BLAST | 170 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 113 | Necessary for association with the ribosome and for stimulation of GTPase activity by 30S ribosomes1 PublicationAdd BLAST | 113 | |
Regioni | 1 – 20 | Necessary for GMP-PNP-dependent association with the 30S ribosomal subunit2 Publications1 PublicationAdd BLAST | 20 | |
Regioni | 287 – 319 | Required for binding to mature and immature 30S ribosomes1 PublicationAdd BLAST | 33 | |
Regioni | 320 – 350 | Required to remove RbfA from mature 30S ribosomes1 PublicationAdd BLAST | 31 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1162, Bacteria |
HOGENOMi | CLU_033617_2_0_6 |
InParanoidi | P39286 |
PhylomeDBi | P39286 |
Family and domain databases
CDDi | cd01854, YjeQ_EngC, 1 hit |
HAMAPi | MF_01820, GTPase_RsgA, 1 hit |
InterProi | View protein in InterPro IPR030378, G_CP_dom IPR027417, P-loop_NTPase IPR004881, Ribosome_biogen_GTPase_RsgA IPR010914, RsgA_GTPase_dom |
PANTHERi | PTHR32120, PTHR32120, 1 hit |
Pfami | View protein in Pfam PF03193, RsgA_GTPase, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00157, TIGR00157, 1 hit |
PROSITEi | View protein in PROSITE PS50936, ENGC_GTPASE, 1 hit PS51721, G_CP, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSKNKLSKGQ QRRVNANHQR RLKTSKEKPD YDDNLFGEPD EGIVISRFGM
60 70 80 90 100
HADVESADGD VHRCNIRRTI RSLVTGDRVV WRPGKPAAEG VNVKGIVEAV
110 120 130 140 150
HERTSVLTRP DFYDGVKPIA ANIDQIVIVS AILPELSLNI IDRYLVACET
160 170 180 190 200
LQIEPIIVLN KIDLLDDEGM AFVNEQMDIY RNIGYRVLMV SSHTQDGLKP
210 220 230 240 250
LEEALTGRIS IFAGQSGVGK SSLLNALLGL QKEILTNDIS DNSGLGQHTT
260 270 280 290 300
TAARLYHFPH GGDVIDSPGV REFGLWHLEP EQITQGFVEF HDYLGLCKYR
310 320 330 340 350
DCKHDTDPGC AIREAVEEGK IAETRFENYH RILESMAQVK TRKNFSDTDD
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97060.1 Different initiation. U00096 Genomic DNA Translation: AAC77121.2 AP009048 Genomic DNA Translation: BAE78165.1 |
PIRi | S56389 |
RefSeqi | NP_418585.4, NC_000913.3 WP_000041964.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77121; AAC77121; b4161 BAE78165; BAE78165; BAE78165 |
GeneIDi | 58390199 948674 |
KEGGi | ecj:JW4122 eco:b4161 |
PATRICi | fig|1411691.4.peg.2537 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97060.1 Different initiation. U00096 Genomic DNA Translation: AAC77121.2 AP009048 Genomic DNA Translation: BAE78165.1 |
PIRi | S56389 |
RefSeqi | NP_418585.4, NC_000913.3 WP_000041964.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YKR | electron microscopy | 9.80 | W | 1-350 | [»] | |
4A2I | electron microscopy | 16.50 | V | 35-337 | [»] | |
5NO2 | electron microscopy | 5.16 | Z | 34-346 | [»] | |
5NO3 | electron microscopy | 5.16 | Z | 34-346 | [»] | |
5NO4 | electron microscopy | 5.16 | Z | 34-346 | [»] | |
5UZ4 | electron microscopy | 5.80 | Z | 6-339 | [»] | |
SMRi | P39286 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261080, 609 interactors |
DIPi | DIP-12581N |
IntActi | P39286, 33 interactors |
MINTi | P39286 |
STRINGi | 511145.b4161 |
Proteomic databases
jPOSTi | P39286 |
PaxDbi | P39286 |
PRIDEi | P39286 |
Genome annotation databases
EnsemblBacteriai | AAC77121; AAC77121; b4161 BAE78165; BAE78165; BAE78165 |
GeneIDi | 58390199 948674 |
KEGGi | ecj:JW4122 eco:b4161 |
PATRICi | fig|1411691.4.peg.2537 |
Organism-specific databases
EchoBASEi | EB2372 |
Phylogenomic databases
eggNOGi | COG1162, Bacteria |
HOGENOMi | CLU_033617_2_0_6 |
InParanoidi | P39286 |
PhylomeDBi | P39286 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7841-MONOMER MetaCyc:G7841-MONOMER |
SABIO-RKi | P39286 |
Miscellaneous databases
EvolutionaryTracei | P39286 |
PROi | PR:P39286 |
Family and domain databases
CDDi | cd01854, YjeQ_EngC, 1 hit |
HAMAPi | MF_01820, GTPase_RsgA, 1 hit |
InterProi | View protein in InterPro IPR030378, G_CP_dom IPR027417, P-loop_NTPase IPR004881, Ribosome_biogen_GTPase_RsgA IPR010914, RsgA_GTPase_dom |
PANTHERi | PTHR32120, PTHR32120, 1 hit |
Pfami | View protein in Pfam PF03193, RsgA_GTPase, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00157, TIGR00157, 1 hit |
PROSITEi | View protein in PROSITE PS50936, ENGC_GTPASE, 1 hit PS51721, G_CP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RSGA_ECOLI | |
Accessioni | P39286Primary (citable) accession number: P39286 Secondary accession number(s): Q2M6E1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | December 15, 1998 | |
Last modified: | April 7, 2021 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families