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UniProtKB - P39285 (MSCM_ECOLI)

Entry version 148 (10 Feb 2021)
Sequence version 3 (15 Jul 1998)
Previous versions | rss
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Protein

Miniconductance mechanosensitive channel MscM

Gene

mscM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Mechanosensitive channel that protects cells against hypoosmotic stress when highly overexpressed. Gates spontaneously in response to increased membrane tension.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionIon channel
Biological processIon transport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:G7840-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		1.A.23.1.3, the small conductance mechanosensitive ion channel (mscs) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Miniconductance mechanosensitive channel MscM
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:mscM
Synonyms:yjeP
Ordered Locus Names:b4159, JW4120
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei467 – 487HelicalSequence analysisAdd BLAST21
Transmembranei522 – 542HelicalSequence analysisAdd BLAST21
Transmembranei551 – 571HelicalSequence analysisAdd BLAST21
Transmembranei600 – 620HelicalSequence analysisAdd BLAST21
Transmembranei628 – 648HelicalSequence analysisAdd BLAST21
Transmembranei674 – 694HelicalSequence analysisAdd BLAST21
Transmembranei698 – 718HelicalSequence analysisAdd BLAST21
Transmembranei785 – 805HelicalSequence analysisAdd BLAST21
Transmembranei828 – 848HelicalSequence analysisAdd BLAST21
Transmembranei875 – 895HelicalSequence analysisAdd BLAST21
Transmembranei910 – 930HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section denotes the presence of an N-terminal signal peptide.<p><a href='/help/signal' target='_top'>More...</a></p>Signal peptidei1 – 19Sequence analysisAdd BLAST19
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000003618020 – 1107Miniconductance mechanosensitive channel MscMAdd BLAST1088

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P39285

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P39285

PRoteomics IDEntifications database

More...PRIDEi		P39285

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homoheptamer.

1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4262029, 305 interactors

Protein interaction database and analysis system

More...IntActi		P39285, 3 interactors

STRING: functional protein association networks

More...STRINGi		511145.b4159

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P39285

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the MscS (TC 1.A.23) family. [View classification]Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG1511, Bacteria
COG3264, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_007829_2_0_6

Database for complete collections of gene phylogenies

More...PhylomeDBi		P39285

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.30.30.60, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR010920, LSM_dom_sf
IPR006685, MscS_channel
IPR011066, MscS_channel_C
IPR006686, MscS_channel_CS
IPR011014, MscS_channel_TM-2
IPR023408, MscS_dom_sf
IPR025692, MscS_IM_dom1
IPR024393, MscS_porin

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00924, MS_channel, 1 hit
PF12795, MscS_porin, 1 hit
PF12794, MscS_TM, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF50182, SSF50182, 1 hit
SSF82689, SSF82689, 1 hit
SSF82861, SSF82861, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS01246, UPF0003, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P39285-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLIITFLMA WCLSWGAYAA TAPDSKQITQ ELEQAKAAKP AQPEVVEALQ 
        60         70         80         90        100
SALNALEERK GSLERIKQYQ QVIDNYPKLS ATLRAQLNNM RDEPRSVSPG 
       110        120        130        140        150
MSTDALNQEI LQVSSQLLDK SRQAQQEQER AREIADSLNQ LPQQQTDARR 
       160        170        180        190        200
QLNEIERRLG TLTGNTPLNQ AQNFALQSDS ARLKALVDEL ELAQLSANNR 
       210        220        230        240        250
QELARLRSEL AEKESQQLDA YLQALRNQLN SQRQLEAERA LESTELLAEN 
       260        270        280        290        300
SADLPKDIVA QFKINRELSA ALNQQAQRMD LVASQQRQAA SQTLQVRQAL 
       310        320        330        340        350
NTLREQSQWL GSSNLLGEAL RAQVARLPEM PKPQQLDTEM AQLRVQRLRY 
       360        370        380        390        400
EDLLNKQPLL RQIHQADGQP LTAEQNRILE AQLRTQRELL NSLLQGGDTL 
       410        420        430        440        450
LLELTKLKVS NGQLEDALKE VNEATHRYLF WTSDVRPMTI AWPLEIAQDL 
       460        470        480        490        500
RRLISLDTFS QLGKASVMML TSKETILPLF GALILVGCSI YSRRYFTRFL 
       510        520        530        540        550
ERSAAKVGKV TQDHFWLTLR TLFWSILVAS PLPVLWMTLG YGLREAWPYP 
       560        570        580        590        600
LAVAIGDGVT ATVPLLWVVM ICATFARPNG LFIAHFGWPR ERVSRGMRYY 
       610        620        630        640        650
LMSIGLIVPL IMALMMFDNL DDREFSGSLG RLCFILICGA LAVVTLSLKK 
       660        670        680        690        700
AGIPLYLNKE GSGDNITNHM LWNMMIGAPL VAILASAVGY LATAQALLAR 
       710        720        730        740        750
LETSVAIWFL LLVVYHVIRR WMLIQRRRLA FDRAKHRRAE MLAQRARGEE 
       760        770        780        790        800
EAHHHSSPEG AIEVDESEVD LDAISAQSLR LVRSILMLIA LLSVIVLWSE 
       810        820        830        840        850
IHSAFGFLEN ISLWDVTSTV QGVESLEPIT LGAVLIAILV FIITTQLVRN 
       860        870        880        890        900
LPALLELAIL QHLDLTPGTG YAITTITKYL LMLIGGLVGF SMIGIEWSKL 
       910        920        930        940        950
QWLVAALGVG LGFGLQEIFA NFISGLIILF EKPIRIGDTV TIRDLTGSVT 
       960        970        980        990       1000
KINTRATTIS DWDRKEIIVP NKAFITEQFI NWSLSDSVTR VVLTIPAPAD 
      1010       1020       1030       1040       1050
ANSEEVTEIL LTAARRCSLV IDNPAPEVFL VDLQQGIQIF ELRIYAAEMG 
      1060       1070       1080       1090       1100
HRMPLRHEIH QLILAGFHAH GIDMPFPPFQ MRLESLNGKQ TGRTLTSAGK 

GRQAGSL
Length:1,107
Mass (Da):123,968
Last modified:July 15, 1998 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5F52A2993B90532B
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA83897 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti1015R → A in AAA97058 (PubMed:7610040).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97058.1
U00096 Genomic DNA Translation: AAC77119.1
AP009048 Genomic DNA Translation: BAE78163.1
J03916 Genomic DNA Translation: AAA83897.1 Different initiation.

Protein sequence database of the Protein Information Resource

More...PIRi		E65226

NCBI Reference Sequences

More...RefSeqi		NP_418583.1, NC_000913.3
WP_001236847.1, NZ_STEB01000014.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC77119; AAC77119; b4159
BAE78163; BAE78163; BAE78163

Database of genes from NCBI RefSeq genomes

More...GeneIDi		57730055
948676

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW4120
eco:b4159

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.2539

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97058.1
U00096 Genomic DNA Translation: AAC77119.1
AP009048 Genomic DNA Translation: BAE78163.1
J03916 Genomic DNA Translation: AAA83897.1 Different initiation.
PIRiE65226
RefSeqiNP_418583.1, NC_000913.3
WP_001236847.1, NZ_STEB01000014.1

3D structure databases

SMRiP39285
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4262029, 305 interactors
IntActiP39285, 3 interactors
STRINGi511145.b4159

Protein family/group databases

TCDBi1.A.23.1.3, the small conductance mechanosensitive ion channel (mscs) family

Proteomic databases

jPOSTiP39285
PaxDbiP39285
PRIDEiP39285

Genome annotation databases

EnsemblBacteriaiAAC77119; AAC77119; b4159
BAE78163; BAE78163; BAE78163
GeneIDi57730055
948676
KEGGiecj:JW4120
eco:b4159
PATRICifig|1411691.4.peg.2539

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB2371

Phylogenomic databases

eggNOGiCOG1511, Bacteria
COG3264, Bacteria
HOGENOMiCLU_007829_2_0_6
PhylomeDBiP39285

Enzyme and pathway databases

BioCyciEcoCyc:G7840-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P39285

Family and domain databases

Gene3Di2.30.30.60, 1 hit
InterProiView protein in InterPro
IPR010920, LSM_dom_sf
IPR006685, MscS_channel
IPR011066, MscS_channel_C
IPR006686, MscS_channel_CS
IPR011014, MscS_channel_TM-2
IPR023408, MscS_dom_sf
IPR025692, MscS_IM_dom1
IPR024393, MscS_porin
PfamiView protein in Pfam
PF00924, MS_channel, 1 hit
PF12795, MscS_porin, 1 hit
PF12794, MscS_TM, 1 hit
SUPFAMiSSF50182, SSF50182, 1 hit
SSF82689, SSF82689, 1 hit
SSF82861, SSF82861, 1 hit
PROSITEiView protein in PROSITE
PS01246, UPF0003, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMSCM_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P39285
Secondary accession number(s): P76798, Q2M6E3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: February 10, 2021
This is version 148 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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