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UniProtKB - P39285 (MSCM_ECOLI)
Protein
Miniconductance mechanosensitive channel MscM
Gene
mscM
Organism
Escherichia coli (strain K12)
Status
Functioni
Mechanosensitive channel that protects cells against hypoosmotic stress when highly overexpressed. Gates spontaneously in response to increased membrane tension.
1 PublicationGO - Biological processi
- ion transport Source: UniProtKB-KW
- transmembrane transport Source: InterPro
Keywordsi
Molecular function | Ion channel |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:G7840-MONOMER |
Protein family/group databases
TCDBi | 1.A.23.1.3, the small conductance mechanosensitive ion channel (mscs) family |
Names & Taxonomyi
Protein namesi | Recommended name: Miniconductance mechanosensitive channel MscM |
Gene namesi | Name:mscM Synonyms:yjeP Ordered Locus Names:b4159, JW4120 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 467 – 487 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 522 – 542 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 551 – 571 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 600 – 620 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 628 – 648 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 674 – 694 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 698 – 718 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 785 – 805 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 828 – 848 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 875 – 895 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 910 – 930 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000036180 | 20 – 1107 | Miniconductance mechanosensitive channel MscMAdd BLAST | 1088 |
Proteomic databases
jPOSTi | P39285 |
PaxDbi | P39285 |
PRIDEi | P39285 |
Interactioni
Subunit structurei
Homoheptamer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262029, 305 interactors |
IntActi | P39285, 3 interactors |
STRINGi | 511145.b4159 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1511, Bacteria COG3264, Bacteria |
HOGENOMi | CLU_007829_2_0_6 |
OMAi | MALITFD |
PhylomeDBi | P39285 |
Family and domain databases
Gene3Di | 2.30.30.60, 1 hit |
InterProi | View protein in InterPro IPR010920, LSM_dom_sf IPR006685, MscS_channel IPR011066, MscS_channel_C IPR006686, MscS_channel_CS IPR011014, MscS_channel_TM-2 IPR023408, MscS_dom_sf IPR025692, MscS_IM_dom1 IPR024393, MscS_porin |
Pfami | View protein in Pfam PF00924, MS_channel, 1 hit PF12795, MscS_porin, 1 hit PF12794, MscS_TM, 1 hit |
SUPFAMi | SSF50182, SSF50182, 1 hit SSF82689, SSF82689, 1 hit SSF82861, SSF82861, 1 hit |
PROSITEi | View protein in PROSITE PS01246, UPF0003, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P39285-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLIITFLMA WCLSWGAYAA TAPDSKQITQ ELEQAKAAKP AQPEVVEALQ
60 70 80 90 100
SALNALEERK GSLERIKQYQ QVIDNYPKLS ATLRAQLNNM RDEPRSVSPG
110 120 130 140 150
MSTDALNQEI LQVSSQLLDK SRQAQQEQER AREIADSLNQ LPQQQTDARR
160 170 180 190 200
QLNEIERRLG TLTGNTPLNQ AQNFALQSDS ARLKALVDEL ELAQLSANNR
210 220 230 240 250
QELARLRSEL AEKESQQLDA YLQALRNQLN SQRQLEAERA LESTELLAEN
260 270 280 290 300
SADLPKDIVA QFKINRELSA ALNQQAQRMD LVASQQRQAA SQTLQVRQAL
310 320 330 340 350
NTLREQSQWL GSSNLLGEAL RAQVARLPEM PKPQQLDTEM AQLRVQRLRY
360 370 380 390 400
EDLLNKQPLL RQIHQADGQP LTAEQNRILE AQLRTQRELL NSLLQGGDTL
410 420 430 440 450
LLELTKLKVS NGQLEDALKE VNEATHRYLF WTSDVRPMTI AWPLEIAQDL
460 470 480 490 500
RRLISLDTFS QLGKASVMML TSKETILPLF GALILVGCSI YSRRYFTRFL
510 520 530 540 550
ERSAAKVGKV TQDHFWLTLR TLFWSILVAS PLPVLWMTLG YGLREAWPYP
560 570 580 590 600
LAVAIGDGVT ATVPLLWVVM ICATFARPNG LFIAHFGWPR ERVSRGMRYY
610 620 630 640 650
LMSIGLIVPL IMALMMFDNL DDREFSGSLG RLCFILICGA LAVVTLSLKK
660 670 680 690 700
AGIPLYLNKE GSGDNITNHM LWNMMIGAPL VAILASAVGY LATAQALLAR
710 720 730 740 750
LETSVAIWFL LLVVYHVIRR WMLIQRRRLA FDRAKHRRAE MLAQRARGEE
760 770 780 790 800
EAHHHSSPEG AIEVDESEVD LDAISAQSLR LVRSILMLIA LLSVIVLWSE
810 820 830 840 850
IHSAFGFLEN ISLWDVTSTV QGVESLEPIT LGAVLIAILV FIITTQLVRN
860 870 880 890 900
LPALLELAIL QHLDLTPGTG YAITTITKYL LMLIGGLVGF SMIGIEWSKL
910 920 930 940 950
QWLVAALGVG LGFGLQEIFA NFISGLIILF EKPIRIGDTV TIRDLTGSVT
960 970 980 990 1000
KINTRATTIS DWDRKEIIVP NKAFITEQFI NWSLSDSVTR VVLTIPAPAD
1010 1020 1030 1040 1050
ANSEEVTEIL LTAARRCSLV IDNPAPEVFL VDLQQGIQIF ELRIYAAEMG
1060 1070 1080 1090 1100
HRMPLRHEIH QLILAGFHAH GIDMPFPPFQ MRLESLNGKQ TGRTLTSAGK
GRQAGSL
Sequence cautioni
The sequence AAA83897 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1015 | R → A in AAA97058 (PubMed:7610040).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97058.1 U00096 Genomic DNA Translation: AAC77119.1 AP009048 Genomic DNA Translation: BAE78163.1 J03916 Genomic DNA Translation: AAA83897.1 Different initiation. |
PIRi | E65226 |
RefSeqi | NP_418583.1, NC_000913.3 WP_001236847.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77119; AAC77119; b4159 BAE78163; BAE78163; BAE78163 |
GeneIDi | 948676 |
KEGGi | ecj:JW4120 eco:b4159 |
PATRICi | fig|1411691.4.peg.2539 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97058.1 U00096 Genomic DNA Translation: AAC77119.1 AP009048 Genomic DNA Translation: BAE78163.1 J03916 Genomic DNA Translation: AAA83897.1 Different initiation. |
PIRi | E65226 |
RefSeqi | NP_418583.1, NC_000913.3 WP_001236847.1, NZ_STEB01000014.1 |
3D structure databases
AlphaFoldDBi | P39285 |
SMRi | P39285 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262029, 305 interactors |
IntActi | P39285, 3 interactors |
STRINGi | 511145.b4159 |
Protein family/group databases
TCDBi | 1.A.23.1.3, the small conductance mechanosensitive ion channel (mscs) family |
Proteomic databases
jPOSTi | P39285 |
PaxDbi | P39285 |
PRIDEi | P39285 |
Genome annotation databases
EnsemblBacteriai | AAC77119; AAC77119; b4159 BAE78163; BAE78163; BAE78163 |
GeneIDi | 948676 |
KEGGi | ecj:JW4120 eco:b4159 |
PATRICi | fig|1411691.4.peg.2539 |
Organism-specific databases
EchoBASEi | EB2371 |
Phylogenomic databases
eggNOGi | COG1511, Bacteria COG3264, Bacteria |
HOGENOMi | CLU_007829_2_0_6 |
OMAi | MALITFD |
PhylomeDBi | P39285 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7840-MONOMER |
Miscellaneous databases
PROi | PR:P39285 |
Family and domain databases
Gene3Di | 2.30.30.60, 1 hit |
InterProi | View protein in InterPro IPR010920, LSM_dom_sf IPR006685, MscS_channel IPR011066, MscS_channel_C IPR006686, MscS_channel_CS IPR011014, MscS_channel_TM-2 IPR023408, MscS_dom_sf IPR025692, MscS_IM_dom1 IPR024393, MscS_porin |
Pfami | View protein in Pfam PF00924, MS_channel, 1 hit PF12795, MscS_porin, 1 hit PF12794, MscS_TM, 1 hit |
SUPFAMi | SSF50182, SSF50182, 1 hit SSF82689, SSF82689, 1 hit SSF82861, SSF82861, 1 hit |
PROSITEi | View protein in PROSITE PS01246, UPF0003, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MSCM_ECOLI | |
Accessioni | P39285Primary (citable) accession number: P39285 Secondary accession number(s): P76798, Q2M6E3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | July 15, 1998 | |
Last modified: | May 25, 2022 | |
This is version 150 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families