We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - P39280 (EPMB_ECOLI)
Protein
L-lysine 2,3-aminomutase
Gene
epmB
Organism
Escherichia coli (strain K12)
Status
Functioni
With EpmA is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF-P) on 'Lys-34'. EpmB appears to act before EpmA. Displays lysine 2,3-aminomutase activity, producing (R)-beta-lysine from (S)-alpha-lysine (L-lysine). Cannot use (S)-ornithine or (R)-alpha-lysine as a substrate.
3 PublicationsCatalytic activityi
Cofactori
Protein has several cofactor binding sites:- [4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication
- pyridoxal 5'-phosphate1 Publication
Kineticsi
kcat is 4.8 min(-1) (at pH 8 and 25 degrees Celsius).
- KM=5 mM for L-lysine (at pH 8 and 25 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 120 | Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis | 1 | |
Metal bindingi | 124 | Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis | 1 | |
Metal bindingi | 127 | Iron-sulfur (4Fe-4S-S-AdoMet)Sequence analysis | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- intramolecular transferase activity, transferring amino groups Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
Keywordsi
Molecular function | Isomerase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:G7836-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: L-lysine 2,3-aminomutase (EC:5.4.3.-)Short name: LAM Alternative name(s): EF-P post-translational modification enzyme B |
Gene namesi | Name:epmB Synonyms:yjeK Ordered Locus Names:b4146, JW4106 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lack CadA activity (lysine decarboxylase).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000172288 | 1 – 342 | L-lysine 2,3-aminomutaseAdd BLAST | 342 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 332 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
jPOSTi | P39280 |
PaxDbi | P39280 |
PRIDEi | P39280 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 852954, 1 interactor |
IntActi | P39280, 16 interactors |
STRINGi | 511145.b4146 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 106 – 329 | Radical SAM corePROSITE-ProRule annotationAdd BLAST | 224 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1509, Bacteria |
HOGENOMi | CLU_032161_2_0_6 |
InParanoidi | P39280 |
OMAi | PIWLNTH |
PhylomeDBi | P39280 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR022462, EpmB IPR003739, Lys_aminomutase/Glu_NH3_mut IPR007197, rSAM |
PANTHERi | PTHR30538, PTHR30538, 1 hit |
Pfami | View protein in Pfam PF04055, Radical_SAM, 1 hit |
PIRSFi | PIRSF004911, DUF160, 1 hit |
SFLDi | SFLDF00314, L-lysine_2_3-aminomutase_(yjeK, 1 hit SFLDS00029, Radical_SAM, 1 hit |
TIGRFAMsi | TIGR03821, EFP_modif_epmB, 1 hit TIGR00238, TIGR00238, 1 hit |
PROSITEi | View protein in PROSITE PS51918, RADICAL_SAM, 1 hit |
i Sequence
Sequence statusi: Complete.
P39280-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAHIVTLNTP SREDWLTQLA DVVTDPDELL RLLNIDAEEK LLAGRSAKKL
60 70 80 90 100
FALRVPRSFI DRMEKGNPDD PLLRQVLTSQ DEFVIAPGFS TDPLEEQHSV
110 120 130 140 150
VPGLLHKYHN RALLLVKGGC AVNCRYCFRR HFPYAENQGN KRNWQTALEY
160 170 180 190 200
VAAHPELDEM IFSGGDPLMA KDHELDWLLT QLEAIPHIKR LRIHSRLPIV
210 220 230 240 250
IPARITEALV ECFARSTLQI LLVNHINHAN EVDETFRQAM AKLRRVGVTL
260 270 280 290 300
LNQSVLLRDV NDNAQTLANL SNALFDAGVM PYYLHVLDKV QGAAHFMVSD
310 320 330 340
DEARQIMREL LTLVSGYLVP KLAREIGGEP SKTPLDLQLR QQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97045.1 U00096 Genomic DNA Translation: AAC77106.1 AP009048 Genomic DNA Translation: BAE78148.1 |
PIRi | S56374 |
RefSeqi | NP_418570.1, NC_000913.3 WP_000940549.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77106; AAC77106; b4146 BAE78148; BAE78148; BAE78148 |
GeneIDi | 948662 |
KEGGi | ecj:JW4106 eco:b4146 |
PATRICi | fig|1411691.4.peg.2554 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97045.1 U00096 Genomic DNA Translation: AAC77106.1 AP009048 Genomic DNA Translation: BAE78148.1 |
PIRi | S56374 |
RefSeqi | NP_418570.1, NC_000913.3 WP_000940549.1, NZ_LN832404.1 |
3D structure databases
AlphaFoldDBi | P39280 |
SMRi | P39280 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 852954, 1 interactor |
IntActi | P39280, 16 interactors |
STRINGi | 511145.b4146 |
Proteomic databases
jPOSTi | P39280 |
PaxDbi | P39280 |
PRIDEi | P39280 |
Genome annotation databases
EnsemblBacteriai | AAC77106; AAC77106; b4146 BAE78148; BAE78148; BAE78148 |
GeneIDi | 948662 |
KEGGi | ecj:JW4106 eco:b4146 |
PATRICi | fig|1411691.4.peg.2554 |
Organism-specific databases
EchoBASEi | EB2366 |
Phylogenomic databases
eggNOGi | COG1509, Bacteria |
HOGENOMi | CLU_032161_2_0_6 |
InParanoidi | P39280 |
OMAi | PIWLNTH |
PhylomeDBi | P39280 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7836-MONOMER |
Miscellaneous databases
PROi | PR:P39280 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR022462, EpmB IPR003739, Lys_aminomutase/Glu_NH3_mut IPR007197, rSAM |
PANTHERi | PTHR30538, PTHR30538, 1 hit |
Pfami | View protein in Pfam PF04055, Radical_SAM, 1 hit |
PIRSFi | PIRSF004911, DUF160, 1 hit |
SFLDi | SFLDF00314, L-lysine_2_3-aminomutase_(yjeK, 1 hit SFLDS00029, Radical_SAM, 1 hit |
TIGRFAMsi | TIGR03821, EFP_modif_epmB, 1 hit TIGR00238, TIGR00238, 1 hit |
PROSITEi | View protein in PROSITE PS51918, RADICAL_SAM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EPMB_ECOLI | |
Accessioni | P39280Primary (citable) accession number: P39280 Secondary accession number(s): Q2M6F8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | May 25, 2022 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families