UniProtKB - P39277 (YJEH_ECOLI)
Protein
L-methionine/branched-chain amino acid exporter YjeH
Gene
yjeH
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the efflux of L-methionine. Can also export L-leucine, L-isoleucine and L-valine. Activity is dependent on electrochemical potential.1 Publication
Activity regulationi
Efflux is inhibited by carbonyl cyanide m-chlorophenylhydrazone (CCCP).1 Publication
GO - Molecular functioni
- L-methionine secondary active transmembrane transporter activity Source: EcoCyc
- neutral L-amino acid secondary active transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- cellular response to amino acid stimulus Source: EcoCyc
- isoleucine transmembrane transport Source: EcoCyc
- leucine transport Source: EcoCyc
- L-valine transmembrane transport Source: EcoCyc
- methionine transport Source: EcoCyc
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:B4141-MONOMER ECOL316407:JW4101-MONOMER MetaCyc:B4141-MONOMER |
Protein family/group databases
TCDBi | 2.A.3.13.1 the amino acid-polyamine-organocation (apc) family |
Names & Taxonomyi
Protein namesi | Recommended name: L-methionine/branched-chain amino acid exporter YjeHCurated |
Gene namesi | Name:yjeH Ordered Locus Names:b4141, JW4101 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 15 | PeriplasmicCuratedAdd BLAST | 15 | |
Transmembranei | 16 – 36 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 37 – 41 | CytoplasmicCurated | 5 | |
Transmembranei | 42 – 62 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 63 – 89 | PeriplasmicCuratedAdd BLAST | 27 | |
Transmembranei | 90 – 110 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 111 – 113 | CytoplasmicCurated | 3 | |
Transmembranei | 114 – 134 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 135 – 147 | PeriplasmicCuratedAdd BLAST | 13 | |
Transmembranei | 148 – 168 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 169 – 182 | CytoplasmicCuratedAdd BLAST | 14 | |
Transmembranei | 183 – 203 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 204 – 219 | PeriplasmicCuratedAdd BLAST | 16 | |
Transmembranei | 220 – 240 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 241 – 257 | CytoplasmicCuratedAdd BLAST | 17 | |
Transmembranei | 258 – 278 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 279 – 317 | PeriplasmicCuratedAdd BLAST | 39 | |
Transmembranei | 318 – 338 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 339 – 341 | CytoplasmicCurated | 3 | |
Transmembranei | 342 – 362 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 363 – 378 | PeriplasmicCuratedAdd BLAST | 16 | |
Transmembranei | 379 – 399 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 400 – 418 | Cytoplasmic1 PublicationAdd BLAST | 19 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Deletion increases the susceptibility to DL-ethionine, DL-norleucine and DL-norvaline. Deletion also results in intracellular accumulation and a reduced rate of export of methionine in the presence of extracellular Met-Met dipeptides.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 | T → Y: Strong decrease in methionine efflux. 1 Publication | 1 | |
Mutagenesisi | 25 | G → F: Strong decrease in methionine efflux. 1 Publication | 1 | |
Mutagenesisi | 195 | W → A: Strong decrease in methionine efflux. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169738 | 1 – 418 | L-methionine/branched-chain amino acid exporter YjeHAdd BLAST | 418 |
Proteomic databases
PaxDbi | P39277 |
PRIDEi | P39277 |
Expressioni
Inductioni
Induced in the presence of intracellular L-methionine, L-leucine or L-isoleucine.1 Publication
Interactioni
Protein-protein interaction databases
BioGridi | 4262197, 12 interactors 852948, 1 interactor |
DIPi | DIP-12573N |
IntActi | P39277, 4 interactors |
STRINGi | 511145.b4141 |
Family & Domainsi
Sequence similaritiesi
Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid efflux (AAE) (TC 2.A.3.13) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG4105DVH Bacteria COG0531 LUCA |
HOGENOMi | HOG000273866 |
InParanoidi | P39277 |
KOi | K16263 |
PhylomeDBi | P39277 |
Family and domain databases
InterProi | View protein in InterPro IPR002293 AA/rel_permease1 |
Pfami | View protein in Pfam PF13520 AA_permease_2, 1 hit |
PIRSFi | PIRSF006060 AA_transporter, 1 hit |
i Sequence
Sequence statusi: Complete.
P39277-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGLKQELGL AQGIGLLSTS LLGTGVFAVP ALAALVAGNN SLWAWPVLII
60 70 80 90 100
LVFPIAIVFA ILGRHYPSAG GVAHFVGMAF GSRLERVTGW LFLSVIPVGL
110 120 130 140 150
PAALQIAAGF GQAMFGWHSW QLLLAELGTL ALVWYIGTRG ASSSANLQTV
160 170 180 190 200
IAGLIVALIV AIWWAGDIKP ANIPFPAPGN IELTGLFAAL SVMFWCFVGL
210 220 230 240 250
EAFAHLASEF KNPERDFPRA LMIGLLLAGL VYWGCTVVVL HFDAYGEKMA
260 270 280 290 300
AAASLPKIVV QLFGVGALWI ACVIGYLACF ASLNIYIQSF ARLVWSQAQH
310 320 330 340 350
NPDHYLARLS SRHIPNNALN AVLGCCVVST LVIHALEINL DALIIYANGI
360 370 380 390 400
FIMIYLLCML AGCKLLQGRY RLLAVVGGLL CVLLLAMVGW KSLYALIMLA
410
GLWLLLPKRK TPENGITT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97040.1 U00096 Genomic DNA Translation: AAC77101.1 AP009048 Genomic DNA Translation: BAE78143.1 |
PIRi | S56369 |
RefSeqi | NP_418565.1, NC_000913.3 WP_000015837.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77101; AAC77101; b4141 BAE78143; BAE78143; BAE78143 |
GeneIDi | 948656 |
KEGGi | ecj:JW4101 eco:b4141 |
PATRICi | fig|1411691.4.peg.2559 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97040.1 U00096 Genomic DNA Translation: AAC77101.1 AP009048 Genomic DNA Translation: BAE78143.1 |
PIRi | S56369 |
RefSeqi | NP_418565.1, NC_000913.3 WP_000015837.1, NZ_STEB01000014.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGridi | 4262197, 12 interactors 852948, 1 interactor |
DIPi | DIP-12573N |
IntActi | P39277, 4 interactors |
STRINGi | 511145.b4141 |
Protein family/group databases
TCDBi | 2.A.3.13.1 the amino acid-polyamine-organocation (apc) family |
Proteomic databases
PaxDbi | P39277 |
PRIDEi | P39277 |
Genome annotation databases
EnsemblBacteriai | AAC77101; AAC77101; b4141 BAE78143; BAE78143; BAE78143 |
GeneIDi | 948656 |
KEGGi | ecj:JW4101 eco:b4141 |
PATRICi | fig|1411691.4.peg.2559 |
Organism-specific databases
EchoBASEi | EB2363 |
Phylogenomic databases
eggNOGi | ENOG4105DVH Bacteria COG0531 LUCA |
HOGENOMi | HOG000273866 |
InParanoidi | P39277 |
KOi | K16263 |
PhylomeDBi | P39277 |
Enzyme and pathway databases
BioCyci | EcoCyc:B4141-MONOMER ECOL316407:JW4101-MONOMER MetaCyc:B4141-MONOMER |
Miscellaneous databases
PROi | PR:P39277 |
Family and domain databases
InterProi | View protein in InterPro IPR002293 AA/rel_permease1 |
Pfami | View protein in Pfam PF13520 AA_permease_2, 1 hit |
PIRSFi | PIRSF006060 AA_transporter, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YJEH_ECOLI | |
Accessioni | P39277Primary (citable) accession number: P39277 Secondary accession number(s): Q2M6G3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | November 13, 2019 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene