UniProtKB - P39276 (DTPC_ECOLI)
Protein
Dipeptide and tripeptide permease C
Gene
dtpC
Organism
Escherichia coli (strain K12)
Status
Functioni
Proton-dependent permease that transports di- and tripeptides. Shows significantly higher specificity towards dipeptides than tripeptides. Has a preference for dipeptides with a C-terminal Lys residue. Can bind Ala-Lys, Lys-Ala, Ala-Ala. Can also transport alanine and trialanine.4 Publications
Kineticsi
- KM=0.07 mM for Ala-Lys1 Publication
- KM=0.51 mM for Lys-Ala1 Publication
pH dependencei
Optimum pH is around 6.5.1 Publication
GO - Molecular functioni
- dipeptide transmembrane transporter activity Source: EcoCyc
- oligopeptide transmembrane transporter activity Source: GO_Central
- peptide:proton symporter activity Source: EcoCyc
- peptide transmembrane transporter activity Source: GO_Central
- proton transmembrane transporter activity Source: EcoCyc
- tripeptide transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- dipeptide transmembrane transport Source: EcoCyc
- protein transport Source: UniProtKB-KW
- proton transmembrane transport Source: EcoCyc
- tripeptide transmembrane transport Source: EcoCyc
Keywordsi
Biological process | Peptide transport, Protein transport, Symport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:YJDL-MONOMER MetaCyc:YJDL-MONOMER |
BRENDAi | 3.6.3.43, 2026 |
Protein family/group databases
TCDBi | 2.A.17.1.5, the proton-dependent oligopeptide transporter (pot/ptr) family |
Names & Taxonomyi
Protein namesi | Recommended name: Dipeptide and tripeptide permease CCuratedAlternative name(s): Dipeptide/tripeptide:H(+) symporter DtpCCurated |
Gene namesi | Name:dtpC1 Publication Synonyms:yjdL Ordered Locus Names:b4130, JW4091 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 12 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 13 – 33 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 34 – 46 | PeriplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 47 – 67 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 68 – 70 | CytoplasmicSequence analysis | 3 | |
Transmembranei | 71 – 93 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 94 – 102 | PeriplasmicSequence analysis | 9 | |
Transmembranei | 103 – 125 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 126 – 140 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 141 – 161 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 162 – 164 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 165 – 185 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 186 – 208 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 209 – 229 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 230 – 234 | PeriplasmicSequence analysis | 5 | |
Transmembranei | 235 – 255 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 256 – 262 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 263 – 283 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 284 – 307 | PeriplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 308 – 328 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 329 – 340 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 341 – 361 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 362 – 375 | PeriplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 376 – 396 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 397 – 406 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 407 – 427 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 428 – 446 | PeriplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 447 – 467 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 468 – 485 | Cytoplasmic1 PublicationAdd BLAST | 18 |
GO - Cellular componenti
- integral component of membrane Source: GO_Central
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 20 | E → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 20 | E → D: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 20 | E → Q: Decrease in activity. Abolishes the pH dependency. 1 Publication | 1 | |
Mutagenesisi | 388 | E → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 388 | E → D: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 388 | E → Q: Decrease in activity. Does not bind Ala-Lys dipeptide. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064330 | 1 – 485 | Dipeptide and tripeptide permease CAdd BLAST | 485 |
Proteomic databases
PaxDbi | P39276 |
PRIDEi | P39276 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4261129, 143 interactors |
STRINGi | 511145.b4130 |
Family & Domainsi
Sequence similaritiesi
Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3104, Bacteria |
HOGENOMi | CLU_004790_0_0_6 |
InParanoidi | P39276 |
PhylomeDBi | P39276 |
Family and domain databases
InterProi | View protein in InterPro IPR005279, Dipep/tripep_permease IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR000109, POT_fam IPR018456, PTR2_symporter_CS |
PANTHERi | PTHR11654, PTHR11654, 1 hit |
Pfami | View protein in Pfam PF00854, PTR2, 1 hit |
SUPFAMi | SSF103473, SSF103473, 2 hits |
TIGRFAMsi | TIGR00924, yjdL_sub1_fam, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS01022, PTR2_1, 1 hit PS01023, PTR2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P39276-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTPSQPRAI YYIVAIQIWE YFSFYGMRAL LILYLTHQLG FDDNHAISLF
60 70 80 90 100
SAYASLVYVT PILGGWLADR LLGNRTAVIA GALLMTLGHV VLGIDTNSTF
110 120 130 140 150
SLYLALAIII CGYGLFKSNI SCLLGELYDE NDHRRDGGFS LLYAAGNIGS
160 170 180 190 200
IAAPIACGLA AQWYGWHVGF ALAGGGMFIG LLIFLSGHRH FQSTRSMDKK
210 220 230 240 250
ALTSVKFALP VWSWLVVMLC LAPVFFTLLL ENDWSGYLLA IVCLIAAQII
260 270 280 290 300
ARMMIKFPEH RRALWQIVLL MFVGTLFWVL AQQGGSTISL FIDRFVNRQA
310 320 330 340 350
FNIEVPTALF QSVNAIAVML AGVVLAWLAS PESRGNSTLR VWLKFAFGLL
360 370 380 390 400
LMACGFMLLA FDARHAAADG QASMGVMISG LALMGFAELF IDPVAIAQIT
410 420 430 440 450
RLKMSGVLTG IYMLATGAVA NWLAGVVAQQ TTESQISGMA IAAYQRFFSQ
460 470 480
MGEWTLACVA IIVVLAFATR FLFSTPTNMI QESND
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97030.1 U00096 Genomic DNA Translation: AAC77091.1 AP009048 Genomic DNA Translation: BAE78133.1 |
PIRi | S56359 |
RefSeqi | NP_418554.1, NC_000913.3 WP_000856829.1, NZ_SSZK01000018.1 |
Genome annotation databases
EnsemblBacteriai | AAC77091; AAC77091; b4130 BAE78133; BAE78133; BAE78133 |
GeneIDi | 57730024 948644 |
KEGGi | ecj:JW4091 eco:b4130 |
PATRICi | fig|511145.12.peg.4262 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97030.1 U00096 Genomic DNA Translation: AAC77091.1 AP009048 Genomic DNA Translation: BAE78133.1 |
PIRi | S56359 |
RefSeqi | NP_418554.1, NC_000913.3 WP_000856829.1, NZ_SSZK01000018.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4261129, 143 interactors |
STRINGi | 511145.b4130 |
Protein family/group databases
TCDBi | 2.A.17.1.5, the proton-dependent oligopeptide transporter (pot/ptr) family |
Proteomic databases
PaxDbi | P39276 |
PRIDEi | P39276 |
Genome annotation databases
EnsemblBacteriai | AAC77091; AAC77091; b4130 BAE78133; BAE78133; BAE78133 |
GeneIDi | 57730024 948644 |
KEGGi | ecj:JW4091 eco:b4130 |
PATRICi | fig|511145.12.peg.4262 |
Organism-specific databases
EchoBASEi | EB2362 |
Phylogenomic databases
eggNOGi | COG3104, Bacteria |
HOGENOMi | CLU_004790_0_0_6 |
InParanoidi | P39276 |
PhylomeDBi | P39276 |
Enzyme and pathway databases
BioCyci | EcoCyc:YJDL-MONOMER MetaCyc:YJDL-MONOMER |
BRENDAi | 3.6.3.43, 2026 |
Miscellaneous databases
PROi | PR:P39276 |
Family and domain databases
InterProi | View protein in InterPro IPR005279, Dipep/tripep_permease IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR000109, POT_fam IPR018456, PTR2_symporter_CS |
PANTHERi | PTHR11654, PTHR11654, 1 hit |
Pfami | View protein in Pfam PF00854, PTR2, 1 hit |
SUPFAMi | SSF103473, SSF103473, 2 hits |
TIGRFAMsi | TIGR00924, yjdL_sub1_fam, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit PS01022, PTR2_1, 1 hit PS01023, PTR2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DTPC_ECOLI | |
Accessioni | P39276Primary (citable) accession number: P39276 Secondary accession number(s): Q2M6H3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 10, 2021 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families