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UniProtKB - P39199 (PRMB_ECOLI)
Protein
50S ribosomal protein L3 glutamine methyltransferase
Gene
prmB
Organism
Escherichia coli (strain K12)
Status
Functioni
Specifically methylates the 50S ribosomal protein L3 on 'Gln-150'. Does not methylate the translation termination release factors RF1 and RF2.
1 PublicationCatalytic activityi
- L-glutaminyl-[ribosomal protein uL3] + S-adenosyl-L-methionine = H+ + N5-methyl-L-glutaminyl-[ribosomal protein uL3] + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.2981 Publication
GO - Molecular functioni
- nucleic acid binding Source: InterPro
- protein-glutamine N-methyltransferase activity Source: EcoCyc
- protein methyltransferase activity Source: UniProtKB
- S-adenosylmethionine-dependent methyltransferase activity Source: UniProtKB
- site-specific DNA-methyltransferase (adenine-specific) activity Source: InterPro
GO - Biological processi
- peptidyl-glutamine methylation Source: EcoCyc
- protein methylation Source: UniProtKB
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12449-MONOMER |
BRENDAi | 2.1.1.298, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: 50S ribosomal protein L3 glutamine methyltransferase (EC:2.1.1.298)Short name: L3 MTase Alternative name(s): N5-glutamine methyltransferase PrmB |
Gene namesi | Name:prmB Synonyms:yfcB Ordered Locus Names:b2330, JW5841 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088003 | 1 – 310 | 50S ribosomal protein L3 glutamine methyltransferaseAdd BLAST | 310 |
Proteomic databases
jPOSTi | P39199 |
PaxDbi | P39199 |
PRIDEi | P39199 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261060, 26 interactors |
DIPi | DIP-11976N |
IntActi | P39199, 8 interactors |
STRINGi | 511145.b2330 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2890, Bacteria |
HOGENOMi | CLU_018398_5_1_6 |
InParanoidi | P39199 |
OMAi | IYYGHGT |
PhylomeDBi | P39199 |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
HAMAPi | MF_02125, L3_methyltr_PrmB, 1 hit |
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR004556, HemK-like IPR017127, Ribosome_L3_Gln-N5_MeTrfase IPR029063, SAM-dependent_MTases IPR007848, Small_mtfrase_dom |
Pfami | View protein in Pfam PF05175, MTS, 1 hit |
PIRSFi | PIRSF037167, Mtase_YfcB_prd, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00536, hemK_fam, 1 hit TIGR03533, L3_gln_methyl, 1 hit |
i Sequence
Sequence statusi: Complete.
P39199-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDKIFVDEAV NELQTIQDML RWSVSRFSAA NIWYGHGTDN PWDEAVQLVL
60 70 80 90 100
PSLYLPLDIP EDMRTARLTS SEKHRIVERV IRRVNERIPV AYLTNKAWFC
110 120 130 140 150
GHEFYVDERV LVPRSPIGEL INNKFAGLIS KQPQHILDMC TGSGCIAIAC
160 170 180 190 200
AYAFPDAEVD AVDISPDALA VAEQNIEEHG LIHNVIPIRS DLFRDLPKVQ
210 220 230 240 250
YDLIVTNPPY VDAEDMSDLP NEYRHEPELG LASGTDGLKL TRRILGNAAD
260 270 280 290 300
YLADDGVLIC EVGNSMVHLM EQYPDVPFTW LEFDNGGDGV FMLTKEQLIA
310
AREHFAIYKD
Sequence cautioni
The sequence M33021 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75390.2 AP009048 Genomic DNA Translation: BAA16188.2 M27714 Genomic DNA No translation available. Y00720 Genomic DNA No translation available. M33021 Genomic DNA No translation available. |
PIRi | H65005 |
RefSeqi | NP_416833.4, NC_000913.3 WP_001300582.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75390; AAC75390; b2330 BAA16188; BAA16188; BAA16188 |
GeneIDi | 946805 |
KEGGi | ecj:JW5841 eco:b2330 |
PATRICi | fig|511145.12.peg.2426 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75390.2 AP009048 Genomic DNA Translation: BAA16188.2 M27714 Genomic DNA No translation available. Y00720 Genomic DNA No translation available. M33021 Genomic DNA No translation available. |
PIRi | H65005 |
RefSeqi | NP_416833.4, NC_000913.3 WP_001300582.1, NZ_LN832404.1 |
3D structure databases
AlphaFoldDBi | P39199 |
SMRi | P39199 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261060, 26 interactors |
DIPi | DIP-11976N |
IntActi | P39199, 8 interactors |
STRINGi | 511145.b2330 |
Proteomic databases
jPOSTi | P39199 |
PaxDbi | P39199 |
PRIDEi | P39199 |
Genome annotation databases
EnsemblBacteriai | AAC75390; AAC75390; b2330 BAA16188; BAA16188; BAA16188 |
GeneIDi | 946805 |
KEGGi | ecj:JW5841 eco:b2330 |
PATRICi | fig|511145.12.peg.2426 |
Organism-specific databases
EchoBASEi | EB2343 |
Phylogenomic databases
eggNOGi | COG2890, Bacteria |
HOGENOMi | CLU_018398_5_1_6 |
InParanoidi | P39199 |
OMAi | IYYGHGT |
PhylomeDBi | P39199 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG12449-MONOMER |
BRENDAi | 2.1.1.298, 2026 |
Miscellaneous databases
PROi | PR:P39199 |
Family and domain databases
Gene3Di | 3.40.50.150, 1 hit |
HAMAPi | MF_02125, L3_methyltr_PrmB, 1 hit |
InterProi | View protein in InterPro IPR002052, DNA_methylase_N6_adenine_CS IPR004556, HemK-like IPR017127, Ribosome_L3_Gln-N5_MeTrfase IPR029063, SAM-dependent_MTases IPR007848, Small_mtfrase_dom |
Pfami | View protein in Pfam PF05175, MTS, 1 hit |
PIRSFi | PIRSF037167, Mtase_YfcB_prd, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00536, hemK_fam, 1 hit TIGR03533, L3_gln_methyl, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PRMB_ECOLI | |
Accessioni | P39199Primary (citable) accession number: P39199 Secondary accession number(s): P76939, P78252 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | September 26, 2001 | |
Last modified: | May 25, 2022 | |
This is version 164 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families