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UniProtKB - P39199 (PRMB_ECOLI)

Entry version 164 (25 May 2022)
Sequence version 3 (26 Sep 2001)
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Protein

50S ribosomal protein L3 glutamine methyltransferase

Gene

prmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Specifically methylates the 50S ribosomal protein L3 on 'Gln-150'. Does not methylate the translation termination release factors RF1 and RF2.

1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EG12449-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.1.1.298, 2026

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
50S ribosomal protein L3 glutamine methyltransferase (EC:2.1.1.298)
Short name:
L3 MTase
Alternative name(s):
N5-glutamine methyltransferase PrmB
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:prmB
Synonyms:yfcB
Ordered Locus Names:b2330, JW5841
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000880031 – 31050S ribosomal protein L3 glutamine methyltransferaseAdd BLAST310

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P39199

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P39199

PRoteomics IDEntifications database

More...PRIDEi		P39199

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4261060, 26 interactors

Database of interacting proteins

More...DIPi		DIP-11976N

Protein interaction database and analysis system

More...IntActi		P39199, 8 interactors

STRING: functional protein association networks

More...STRINGi		511145.b2330

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		P39199

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P39199

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the protein N5-glutamine methyltransferase family. PrmB subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG2890, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_018398_5_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P39199

Identification of Orthologs from Complete Genome Data

More...OMAi		IYYGHGT

Database for complete collections of gene phylogenies

More...PhylomeDBi		P39199

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.50.150, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_02125, L3_methyltr_PrmB, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR002052, DNA_methylase_N6_adenine_CS
IPR004556, HemK-like
IPR017127, Ribosome_L3_Gln-N5_MeTrfase
IPR029063, SAM-dependent_MTases
IPR007848, Small_mtfrase_dom

Pfam protein domain database

More...Pfami		View protein in Pfam
PF05175, MTS, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF037167, Mtase_YfcB_prd, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF53335, SSF53335, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00536, hemK_fam, 1 hit
TIGR03533, L3_gln_methyl, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P39199-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDKIFVDEAV NELQTIQDML RWSVSRFSAA NIWYGHGTDN PWDEAVQLVL 
        60         70         80         90        100
PSLYLPLDIP EDMRTARLTS SEKHRIVERV IRRVNERIPV AYLTNKAWFC 
       110        120        130        140        150
GHEFYVDERV LVPRSPIGEL INNKFAGLIS KQPQHILDMC TGSGCIAIAC 
       160        170        180        190        200
AYAFPDAEVD AVDISPDALA VAEQNIEEHG LIHNVIPIRS DLFRDLPKVQ 
       210        220        230        240        250
YDLIVTNPPY VDAEDMSDLP NEYRHEPELG LASGTDGLKL TRRILGNAAD 
       260        270        280        290        300
YLADDGVLIC EVGNSMVHLM EQYPDVPFTW LEFDNGGDGV FMLTKEQLIA 
       310
AREHFAIYKD
Length:310
Mass (Da):35,002
Last modified:September 26, 2001 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3EE8F2D45AFC3760
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence M33021 differs from that shown. Reason: Frameshift.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75390.2
AP009048 Genomic DNA Translation: BAA16188.2
M27714 Genomic DNA No translation available.
Y00720 Genomic DNA No translation available.
M33021 Genomic DNA No translation available.

Protein sequence database of the Protein Information Resource

More...PIRi		H65005

NCBI Reference Sequences

More...RefSeqi		NP_416833.4, NC_000913.3
WP_001300582.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC75390; AAC75390; b2330
BAA16188; BAA16188; BAA16188

Database of genes from NCBI RefSeq genomes

More...GeneIDi		946805

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW5841
eco:b2330

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|511145.12.peg.2426

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75390.2
AP009048 Genomic DNA Translation: BAA16188.2
M27714 Genomic DNA No translation available.
Y00720 Genomic DNA No translation available.
M33021 Genomic DNA No translation available.
PIRiH65005
RefSeqiNP_416833.4, NC_000913.3
WP_001300582.1, NZ_LN832404.1

3D structure databases

AlphaFoldDBiP39199
SMRiP39199
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4261060, 26 interactors
DIPiDIP-11976N
IntActiP39199, 8 interactors
STRINGi511145.b2330

Proteomic databases

jPOSTiP39199
PaxDbiP39199
PRIDEiP39199

Genome annotation databases

EnsemblBacteriaiAAC75390; AAC75390; b2330
BAA16188; BAA16188; BAA16188
GeneIDi946805
KEGGiecj:JW5841
eco:b2330
PATRICifig|511145.12.peg.2426

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB2343

Phylogenomic databases

eggNOGiCOG2890, Bacteria
HOGENOMiCLU_018398_5_1_6
InParanoidiP39199
OMAiIYYGHGT
PhylomeDBiP39199

Enzyme and pathway databases

BioCyciEcoCyc:EG12449-MONOMER
BRENDAi2.1.1.298, 2026

Miscellaneous databases

Protein Ontology

More...PROi		PR:P39199

Family and domain databases

Gene3Di3.40.50.150, 1 hit
HAMAPiMF_02125, L3_methyltr_PrmB, 1 hit
InterProiView protein in InterPro
IPR002052, DNA_methylase_N6_adenine_CS
IPR004556, HemK-like
IPR017127, Ribosome_L3_Gln-N5_MeTrfase
IPR029063, SAM-dependent_MTases
IPR007848, Small_mtfrase_dom
PfamiView protein in Pfam
PF05175, MTS, 1 hit
PIRSFiPIRSF037167, Mtase_YfcB_prd, 1 hit
SUPFAMiSSF53335, SSF53335, 1 hit
TIGRFAMsiTIGR00536, hemK_fam, 1 hit
TIGR03533, L3_gln_methyl, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPRMB_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P39199
Secondary accession number(s): P76939, P78252
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: September 26, 2001
Last modified: May 25, 2022
This is version 164 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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