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Protein

Antigen 43

Gene

flu

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Controls colony form variation and autoaggregation. May function as an adhesin.1 Publication

Enzyme and pathway databases

BioCyciEcoCyc:G7080-MONOMER

Protein family/group databases

TCDBi1.B.12.8.2 the autotransporter-1 (at-1) family

Names & Taxonomyi

Protein namesi
Recommended name:
Antigen 43
Short name:
AG43
Alternative name(s):
Fluffing protein
Cleaved into the following 2 chains:
Gene namesi
Name:flu
Synonyms:yeeQ, yzzX
Ordered Locus Names:b2000, JW1982
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12686 flu

Subcellular locationi

Antigen 43 :
Chain Antigen 43 alpha chain :
Chain Antigen 43 beta chain :

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 521 PublicationAdd BLAST52
ChainiPRO_000038757253 – 1039Antigen 43Add BLAST987
ChainiPRO_000000269653 – 551Antigen 43 alpha chainAdd BLAST499
ChainiPRO_0000002697552 – 1039Antigen 43 beta chainAdd BLAST488

Proteomic databases

PaxDbiP39180
PRIDEiP39180

Interactioni

Subunit structurei

Interaction with TamA of the translocation and assembly module (TAM) initiates insertion in the outer membrane (PubMed:25341963).1 Publication

Protein-protein interaction databases

BioGridi4260664, 6 interactors
DIPiDIP-2892N
IntActiP39180, 4 interactors
STRINGi316385.ECDH10B_2146

Structurei

3D structure databases

ProteinModelPortaliP39180
SMRiP39180
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini737 – 1039AutotransporterPROSITE-ProRule annotationAdd BLAST303

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. The C-terminal beta chain translocator domain inserts in the outer membrane via TamA (PubMed:25341963). This domain probably forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage. Finally, the mature protein remains tightly associated with the bacterium (Probable).Curated1 Publication

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105F4K Bacteria
COG3468 LUCA
HOGENOMiHOG000009569
KOiK12687
OMAiFWDSKKK
PhylomeDBiP39180

Family and domain databases

Gene3Di2.160.20.20, 1 hit
InterProiView protein in InterPro
IPR030930 AIDA
IPR005546 Autotransporte_beta
IPR036709 Autotransporte_beta_dom_sf
IPR024973 ESPR
IPR012332 P22_tailspike-like_C_sf
IPR011050 Pectin_lyase_fold/virulence
PfamiView protein in Pfam
PF16168 AIDA, 3 hits
PF03797 Autotransporter, 1 hit
PF13018 ESPR, 1 hit
SMARTiView protein in SMART
SM00869 Autotransporter, 1 hit
SUPFAMiSSF103515 SSF103515, 1 hit
SSF51126 SSF51126, 2 hits
TIGRFAMsiTIGR04415 O_hepto_targRPT, 5 hits
PROSITEiView protein in PROSITE
PS51208 AUTOTRANSPORTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRHLNTCYR LVWNHMTGAF VVASELARAR GKRGGVAVAL SLAAVTSLPV
60 70 80 90 100
LAADIVVHPG ETVNGGTLAN HDNQIVFGTT NGMTISTGLE YGPDNEANTG
110 120 130 140 150
GQWVQDGGTA NKTTVTSGGL QRVNPGGSVS DTVISAGGGQ SLQGRAVNTT
160 170 180 190 200
LNGGEQWMHE GAIATGTVIN DKGWQVVKPG TVATDTVVNT GAEGGPDAEN
210 220 230 240 250
GDTGQFVRGD AVRTTINKNG RQIVRAEGTA NTTVVYAGGD QTVHGHALDT
260 270 280 290 300
TLNGGYQYVH NGGTASDTVV NSDGWQIVKN GGVAGNTTVN QKGRLQVDAG
310 320 330 340 350
GTATNVTLKQ GGALVTSTAA TVTGINRLGA FSVVEGKADN VVLENGGRLD
360 370 380 390 400
VLTGHTATNT RVDDGGTLDV RNGGTATTVS MGNGGVLLAD SGAAVSGTRS
410 420 430 440 450
DGKAFSIGGG QADALMLEKG SSFTLNAGDT ATDTTVNGGL FTARGGTLAG
460 470 480 490 500
TTTLNNGAIL TLSGKTVNND TLTIREGDAL LQGGSLTGNG SVEKSGSGTL
510 520 530 540 550
TVSNTTLTQK AVNLNEGTLT LNDSTVTTDV IAQRGTALKL TGSTVLNGAI
560 570 580 590 600
DPTNVTLASG ATWNIPDNAT VQSVVDDLSH AGQIHFTSTR TGKFVPATLK
610 620 630 640 650
VKNLNGQNGT ISLRVRPDMA QNNADRLVID GGRATGKTIL NLVNAGNSAS
660 670 680 690 700
GLATSGKGIQ VVEAINGATT EEGAFVQGNR LQAGAFNYSL NRDSDESWYL
710 720 730 740 750
RSENAYRAEV PLYASMLTQA MDYDRIVAGS RSHQTGVNGE NNSVRLSIQG
760 770 780 790 800
GHLGHDNNGG IARGATPESS GSYGFVRLEG DLMRTEVAGM SVTAGVYGAA
810 820 830 840 850
GHSSVDVKDD DGSRAGTVRD DAGSLGGYLN LVHTSSGLWA DIVAQGTRHS
860 870 880 890 900
MKASSDNNDF RARGWGWLGS LETGLPFSIT DNLMLEPQLQ YTWQGLSLDD
910 920 930 940 950
GKDNAGYVKF GHGSAQHVRA GFRLGSHNDM TFGEGTSSRA PLRDSAKHSV
960 970 980 990 1000
SELPVNWWVQ PSVIRTFSSR GDMRVGTSTA GSGMTFSPSQ NGTSLDLQAG
1010 1020 1030
LEARVRENIT LGVQAGYAHS VSGSSAEGYN GQATLNVTF
Length:1,039
Mass (Da):106,825
Last modified:October 11, 2004 - v3
Checksum:iABC16C46C264EAED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61 – 63ETV → TTT AA sequence (PubMed:9298646).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti2K → N in strain: ML 308-225. 1
Natural varianti41 – 42SL → FF in strain: ML 308-225. 2
Natural varianti46T → K in strain: ML 308-225. 1
Natural varianti157W → L in strain: ML 308-225. 1
Natural varianti188V → F in strain: ML 308-225. 1
Natural varianti303 – 305ATN → STI in strain: ML 308-225. 3
Natural varianti320A → T in strain: ML 308-225. 1
Natural varianti372N → Q in strain: ML 308-225. 1
Natural varianti493E → V in strain: ML 308-225. 1
Natural varianti497S → N in strain: ML 308-225. 1
Natural varianti585H → Y in strain: ML 308-225. 1
Natural varianti709E → K in strain: ML 308-225. 1
Natural varianti721M → T in strain: ML 308-225. 1
Natural varianti751 – 753GHL → SHF in strain: ML 308-225. 3
Natural varianti803S → P in strain: ML 308-225. 1
Natural varianti815A → V in strain: ML 308-225. 1
Natural varianti829 – 835LNLVHTS → MNLIYNA in strain: ML 308-225. 7
Natural varianti845 – 847QGT → LGA in strain: ML 308-225. 3
Natural varianti855S → T in strain: ML 308-225. 1
Natural varianti888Q → L in strain: ML 308-225. 1
Natural varianti1025S → I in strain: ML 308-225. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24429 Genomic DNA Translation: AAB47869.1
U00096 Genomic DNA Translation: AAT48141.1
AP009048 Genomic DNA Translation: BAA15825.2
RefSeqiWP_000820410.1, NZ_LN832404.1
YP_026164.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48141; AAT48141; b2000
BAA15825; BAA15825; BAA15825
GeneIDi946540
KEGGiecj:JW1982
eco:b2000
PATRICifig|1411691.4.peg.253

Similar proteinsi

Entry informationi

Entry nameiAG43_ECOLI
AccessioniPrimary (citable) accession number: P39180
Secondary accession number(s): P75614
, P76360, P97241, Q46771
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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