UniProtKB - P39176 (ERFK_ECOLI)
Protein
Probable L,D-transpeptidase ErfK/SrfK
Gene
erfK
Organism
Escherichia coli (strain K12)
Status
Functioni
Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp.1 Publication
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 207 | Sequence analysis | 1 |
GO - Molecular functioni
- peptidoglycan L,D-transpeptidase activity Source: EcoCyc
- transferase activity, transferring glycosyl groups Source: UniProtKB-KW
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan-protein cross-linking Source: EcoCyc
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Hydrolase, Transferase |
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:G7073-MONOMER |
UniPathwayi | UPA00219 |
Protein family/group databases
MEROPSi | C82.A02 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:erfK Synonyms:yeeG, yzzT Ordered Locus Names:b1990, JW1968 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Periplasm Curated
GO - Cellular componenti
- periplasmic space Source: UniProtKB-SubCell
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Disruption phenotypei
Simultaneous disruption of erfK, ybiS, ycfS and ynhG leads to loss of covalent anchoring of the major outer membrane lipoprotein (Lpp, also known as the Braun lipoprotein) to the peptidoglycan. Complementation with erfK restores some of this anchoring.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | 1 PublicationAdd BLAST | 21 | |
ChainiPRO_0000021194 | 22 – 310 | Probable L,D-transpeptidase ErfK/SrfKAdd BLAST | 289 |
Proteomic databases
jPOSTi | P39176 |
PaxDbi | P39176 |
PRIDEi | P39176 |
Interactioni
Subunit structurei
Interacts with DsbG.
1 PublicationBinary interactionsi
P39176
With | #Exp. | IntAct |
---|---|---|
pheS [P08312] | 4 | EBI-555707,EBI-555676 |
Protein-protein interaction databases
BioGRIDi | 4259382, 19 interactors 849652, 1 interactor |
DIPi | DIP-9522N |
IntActi | P39176, 3 interactors |
STRINGi | 511145.b1990 |
Family & Domainsi
Sequence similaritiesi
Belongs to the YkuD family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1376, Bacteria |
HOGENOMi | CLU_046834_0_1_6 |
InParanoidi | P39176 |
PhylomeDBi | P39176 |
Family and domain databases
Gene3Di | 2.40.440.10, 1 hit |
InterProi | View protein in InterPro IPR005490, LD_TPept_cat_dom IPR041597, Ldt_C IPR038063, Transpep_catalytic_dom |
Pfami | View protein in Pfam PF17969, Ldt_C, 1 hit PF03734, YkuD, 1 hit |
SUPFAMi | SSF141523, SSF141523, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P39176-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRRVNILCSF ALLFASHTSL AVTYPLPPEG SRLVGQSFTV TVPDHNTQPL
60 70 80 90 100
ETFAAQYGQG LSNMLEANPG ADVFLPKSGS QLTIPQQLIL PDTVRKGIVV
110 120 130 140 150
NVAEMRLYYY PPDSNTVEVF PIGIGQAGRE TPRNWVTTVE RKQEAPTWTP
160 170 180 190 200
TPNTRREYAK RGESLPAFVP AGPDNPMGLY AIYIGRLYAI HGTNANFGIG
210 220 230 240 250
LRVSQGCIRL RNDDIKYLFD NVPVGTRVQI IDQPVKYTTE PDGSNWLEVH
260 270 280 290 300
EPLSRNRAEY ESDRKVPLPV TPSLRAFING QEVDVNRANA ALQRRSGMPV
310
QISSGSRQMF
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 28 – 29 | PE → TD AA sequence (PubMed:9298646).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33333 Genomic DNA Translation: AAA78909.1 U00096 Genomic DNA Translation: AAC75051.1 AP009048 Genomic DNA Translation: BAA15807.1 |
PIRi | E64963 |
RefSeqi | NP_416494.1, NC_000913.3 |
Genome annotation databases
EnsemblBacteriai | AAC75051; AAC75051; b1990 BAA15807; BAA15807; BAA15807 |
GeneIDi | 945273 |
KEGGi | ecj:JW1968 eco:b1990 |
PATRICi | fig|511145.12.peg.2065 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33333 Genomic DNA Translation: AAA78909.1 U00096 Genomic DNA Translation: AAC75051.1 AP009048 Genomic DNA Translation: BAA15807.1 |
PIRi | E64963 |
RefSeqi | NP_416494.1, NC_000913.3 |
3D structure databases
SMRi | P39176 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259382, 19 interactors 849652, 1 interactor |
DIPi | DIP-9522N |
IntActi | P39176, 3 interactors |
STRINGi | 511145.b1990 |
Protein family/group databases
MEROPSi | C82.A02 |
Proteomic databases
jPOSTi | P39176 |
PaxDbi | P39176 |
PRIDEi | P39176 |
Genome annotation databases
EnsemblBacteriai | AAC75051; AAC75051; b1990 BAA15807; BAA15807; BAA15807 |
GeneIDi | 945273 |
KEGGi | ecj:JW1968 eco:b1990 |
PATRICi | fig|511145.12.peg.2065 |
Organism-specific databases
EchoBASEi | EB2546 |
Phylogenomic databases
eggNOGi | COG1376, Bacteria |
HOGENOMi | CLU_046834_0_1_6 |
InParanoidi | P39176 |
PhylomeDBi | P39176 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:G7073-MONOMER |
Miscellaneous databases
PROi | PR:P39176 |
Family and domain databases
Gene3Di | 2.40.440.10, 1 hit |
InterProi | View protein in InterPro IPR005490, LD_TPept_cat_dom IPR041597, Ldt_C IPR038063, Transpep_catalytic_dom |
Pfami | View protein in Pfam PF17969, Ldt_C, 1 hit PF03734, YkuD, 1 hit |
SUPFAMi | SSF141523, SSF141523, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ERFK_ECOLI | |
Accessioni | P39176Primary (citable) accession number: P39176 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families