UniProtKB - P39109 (YCFI_YEAST)
Protein
Metal resistance protein YCF1
Gene
YCF1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.2 Publications
Miscellaneous
Present with 396 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:7.2.2.21 Publication
- an S-substituted glutathione + ATP + H2O = ADP + an S-substituted glutathione + H+ + phosphate1 PublicationEC:7.6.2.31 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 663 – 670 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 1306 – 1313 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity, coupled to transmembrane movement of substances Source: GO_Central
- ATP binding Source: UniProtKB-KW
- bilirubin transmembrane transporter activity Source: SGD
- glutathione S-conjugate-exporting ATPase activity Source: SGD
GO - Biological processi
- bilirubin transport Source: SGD
- cell redox homeostasis Source: SGD
- glutathione metabolic process Source: SGD
- response to cadmium ion Source: UniProtKB-KW
- response to metal ion Source: SGD
- transmembrane transport Source: SGD
- vacuole fusion, non-autophagic Source: SGD
Keywordsi
Molecular function | Translocase |
Biological process | Cadmium resistance, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:G3O-29733-MONOMER YEAST:G3O-29733-MONOMER |
Reactomei | R-SCE-114608 Platelet degranulation R-SCE-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism R-SCE-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX) R-SCE-2142850 Hyaluronan biosynthesis and export R-SCE-382556 ABC-family proteins mediated transport |
Protein family/group databases
TCDBi | 3.A.1.208.11 the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:YCF1 Ordered Locus Names:YDR135C ORF Names:YD9302.11C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YDR135C |
SGDi | S000002542 YCF1 |
Subcellular locationi
Vacuole
- Vacuole membrane 1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication
Vacuole
- fungal-type vacuole Source: GO_Central
- fungal-type vacuole membrane Source: SGD
- vacuolar membrane Source: GO_Central
Other locations
- integral component of membrane Source: SGD
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 32 | VacuolarSequence analysisAdd BLAST | 32 | |
Transmembranei | 33 – 53 | Helical; Name=1PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 54 – 73 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 74 – 94 | Helical; Name=2PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 95 – 99 | VacuolarSequence analysis | 5 | |
Transmembranei | 100 – 120 | Helical; Name=3PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 121 – 130 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 131 – 151 | Helical; Name=4PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 152 – 169 | VacuolarSequence analysisAdd BLAST | 18 | |
Transmembranei | 170 – 190 | Helical; Name=5PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 191 – 278 | CytoplasmicSequence analysisAdd BLAST | 88 | |
Transmembranei | 279 – 299 | Helical; Name=6PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 300 – 345 | VacuolarSequence analysisAdd BLAST | 46 | |
Transmembranei | 346 – 366 | Helical; Name=7PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 367 – 422 | CytoplasmicSequence analysisAdd BLAST | 56 | |
Transmembranei | 423 – 443 | Helical; Name=8PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 444 – 446 | VacuolarSequence analysis | 3 | |
Transmembranei | 447 – 467 | Helical; Name=9PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 468 – 530 | CytoplasmicSequence analysisAdd BLAST | 63 | |
Transmembranei | 531 – 551 | Helical; Name=10PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 552 – 572 | VacuolarSequence analysisAdd BLAST | 21 | |
Transmembranei | 573 – 593 | Helical; Name=11PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 594 – 943 | CytoplasmicSequence analysisAdd BLAST | 350 | |
Transmembranei | 944 – 964 | Helical; Name=12PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 965 – 1001 | VacuolarSequence analysisAdd BLAST | 37 | |
Transmembranei | 1002 – 1023 | Helical; Name=13PROSITE-ProRule annotationAdd BLAST | 22 | |
Topological domaini | 1024 – 1066 | CytoplasmicSequence analysisAdd BLAST | 43 | |
Transmembranei | 1067 – 1087 | Helical; Name=14PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1088 | VacuolarSequence analysis | 1 | |
Transmembranei | 1089 – 1109 | Helical; Name=15PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1110 – 1180 | CytoplasmicSequence analysisAdd BLAST | 71 | |
Transmembranei | 1181 – 1201 | Helical; Name=16PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1202 – 1205 | VacuolarSequence analysis | 4 | |
Transmembranei | 1206 – 1226 | Helical; Name=17PROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1227 – 1515 | CytoplasmicSequence analysisAdd BLAST | 289 |
Keywords - Cellular componenti
Membrane, VacuolePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 713 | Missing : Loss of function. | 1 | |
Mutagenesisi | 908 | S → A: Loss of function. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093449 | 1 – 1515 | Metal resistance protein YCF1Add BLAST | 1515 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 251 | PhosphoserineCombined sources | 1 | |
Modified residuei | 873 | PhosphoserineCombined sources | 1 | |
Modified residuei | 903 | PhosphoserineCombined sources | 1 | |
Modified residuei | 908 | PhosphoserineCombined sources | 1 | |
Modified residuei | 911 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 914 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P39109 |
PaxDbi | P39109 |
PRIDEi | P39109 |
PTM databases
iPTMneti | P39109 |
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGridi | 32190, 109 interactors |
DIPi | DIP-2612N |
IntActi | P39109, 33 interactors |
MINTi | P39109 |
STRINGi | 4932.YDR135C |
Structurei
3D structure databases
ProteinModelPortali | P39109 |
SMRi | P39109 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 287 – 590 | ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST | 304 | |
Domaini | 626 – 853 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 228 | |
Domaini | 951 – 1235 | ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST | 285 | |
Domaini | 1272 – 1507 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 236 |
Sequence similaritiesi
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification]Curated
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
GeneTreei | ENSGT00940000166156 |
InParanoidi | P39109 |
KOi | K05665 |
OMAi | NEWRVDA |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits |
InterProi | View protein in InterPro IPR003593 AAA+_ATPase IPR011527 ABC1_TM_dom IPR036640 ABC1_TM_sf IPR003439 ABC_transporter-like IPR017871 ABC_transporter_CS IPR027417 P-loop_NTPase |
Pfami | View protein in Pfam PF00664 ABC_membrane, 2 hits PF00005 ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382 AAA, 2 hits |
SUPFAMi | SSF52540 SSF52540, 2 hits SSF90123 SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929 ABC_TM1F, 2 hits PS00211 ABC_TRANSPORTER_1, 2 hits PS50893 ABC_TRANSPORTER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
P39109-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGNLVSWAC KLCRSPEGFG PISFYGDFTQ CFIDGVILNL SAIFMITFGI
60 70 80 90 100
RDLVNLCKKK HSGIKYRRNW IIVSRMALVL LEIAFVSLAS LNISKEEAEN
110 120 130 140 150
FTIVSQYAST MLSLFVALAL HWIEYDRSVV ANTVLLFYWL FETFGNFAKL
160 170 180 190 200
INILIRHTYE GIWYSGQTGF ILTLFQVITC ASILLLEALP KKPLMPHQHI
210 220 230 240 250
HQTLTRRKPN PYDSANIFSR ITFSWMSGLM KTGYEKYLVE ADLYKLPRNF
260 270 280 290 300
SSEELSQKLE KNWENELKQK SNPSLSWAIC RTFGSKMLLA AFFKAIHDVL
310 320 330 340 350
AFTQPQLLRI LIKFVTDYNS ERQDDHSSLQ GFENNHPQKL PIVRGFLIAF
360 370 380 390 400
AMFLVGFTQT SVLHQYFLNV FNTGMYIKSA LTALIYQKSL VLSNEASGLS
410 420 430 440 450
STGDIVNLMS VDVQKLQDLT QWLNLIWSGP FQIIICLYSL YKLLGNSMWV
460 470 480 490 500
GVIILVIMMP LNSFLMRIQK KLQKSQMKYK DERTRVISEI LNNIKSLKLY
510 520 530 540 550
AWEKPYREKL EEVRNNKELK NLTKLGCYMA VTSFQFNIVP FLVSCCTFAV
560 570 580 590 600
FVYTEDRALT TDLVFPALTL FNLLSFPLMI IPMVLNSFIE ASVSIGRLFT
610 620 630 640 650
FFTNEELQPD SVQRLPKVKN IGDVAINIGD DATFLWQRKP EYKVALKNIN
660 670 680 690 700
FQAKKGNLTC IVGKVGSGKT ALLSCMLGDL FRVKGFATVH GSVAYVSQVP
710 720 730 740 750
WIMNGTVKEN ILFGHRYDAE FYEKTIKACA LTIDLAILMD GDKTLVGEKG
760 770 780 790 800
ISLSGGQKAR LSLARAVYAR ADTYLLDDPL AAVDEHVARH LIEHVLGPNG
810 820 830 840 850
LLHTKTKVLA TNKVSALSIA DSIALLDNGE ITQQGTYDEI TKDADSPLWK
860 870 880 890 900
LLNNYGKKNN GKSNEFGDSS ESSVRESSIP VEGELEQLQK LNDLDFGNSD
910 920 930 940 950
AISLRRASDA TLGSIDFGDD ENIAKREHRE QGKVKWNIYL EYAKACNPKS
960 970 980 990 1000
VCVFILFIVI SMFLSVMGNV WLKHWSEVNS RYGSNPNAAR YLAIYFALGI
1010 1020 1030 1040 1050
GSALATLIQT IVLWVFCTIH ASKYLHNLMT NSVLRAPMTF FETTPIGRIL
1060 1070 1080 1090 1100
NRFSNDIYKV DALLGRTFSQ FFVNAVKVTF TITVICATTW QFIFIIIPLS
1110 1120 1130 1140 1150
VFYIYYQQYY LRTSRELRRL DSITRSPIYS HFQETLGGLA TVRGYSQQKR
1160 1170 1180 1190 1200
FSHINQCRID NNMSAFYPSI NANRWLAYRL ELIGSIIILG AATLSVFRLK
1210 1220 1230 1240 1250
QGTLTAGMVG LSLSYALQIT QTLNWIVRMT VEVETNIVSV ERIKEYADLK
1260 1270 1280 1290 1300
SEAPLIVEGH RPPKEWPSQG DIKFNNYSTR YRPELDLVLK HINIHIKPNE
1310 1320 1330 1340 1350
KVGIVGRTGA GKSSLTLALF RMIEASEGNI VIDNIAINEI GLYDLRHKLS
1360 1370 1380 1390 1400
IIPQDSQVFE GTVRENIDPI NQYTDEAIWR ALELSHLKEH VLSMSNDGLD
1410 1420 1430 1440 1450
AQLTEGGGNL SVGQRQLLCL ARAMLVPSKI LVLDEATAAV DVETDKVVQE
1460 1470 1480 1490 1500
TIRTAFKDRT ILTIAHRLNT IMDSDRIIVL DNGKVAEFDS PGQLLSDNKS
1510
LFYSLCMEAG LVNEN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 680 | L → R in AAA50353 (PubMed:7521334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L35237 Genomic DNA Translation: AAA50353.1 Z48179 Genomic DNA Translation: CAA88217.1 BK006938 Genomic DNA Translation: DAA11979.1 |
PIRi | S51863 |
RefSeqi | NP_010419.3, NM_001180442.3 |
Genome annotation databases
EnsemblFungii | YDR135C_mRNA; YDR135C_mRNA; YDR135C |
GeneIDi | 851713 |
KEGGi | sce:YDR135C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L35237 Genomic DNA Translation: AAA50353.1 Z48179 Genomic DNA Translation: CAA88217.1 BK006938 Genomic DNA Translation: DAA11979.1 |
PIRi | S51863 |
RefSeqi | NP_010419.3, NM_001180442.3 |
3D structure databases
ProteinModelPortali | P39109 |
SMRi | P39109 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 32190, 109 interactors |
DIPi | DIP-2612N |
IntActi | P39109, 33 interactors |
MINTi | P39109 |
STRINGi | 4932.YDR135C |
Protein family/group databases
TCDBi | 3.A.1.208.11 the atp-binding cassette (abc) superfamily |
PTM databases
iPTMneti | P39109 |
Proteomic databases
MaxQBi | P39109 |
PaxDbi | P39109 |
PRIDEi | P39109 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
EnsemblFungii | YDR135C_mRNA; YDR135C_mRNA; YDR135C |
GeneIDi | 851713 |
KEGGi | sce:YDR135C |
Organism-specific databases
EuPathDBi | FungiDB:YDR135C |
SGDi | S000002542 YCF1 |
Phylogenomic databases
GeneTreei | ENSGT00940000166156 |
InParanoidi | P39109 |
KOi | K05665 |
OMAi | NEWRVDA |
Enzyme and pathway databases
BioCyci | MetaCyc:G3O-29733-MONOMER YEAST:G3O-29733-MONOMER |
Reactomei | R-SCE-114608 Platelet degranulation R-SCE-196741 Cobalamin (Cbl, vitamin B12) transport and metabolism R-SCE-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX) R-SCE-2142850 Hyaluronan biosynthesis and export R-SCE-382556 ABC-family proteins mediated transport |
Miscellaneous databases
PROi | PR:P39109 |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits |
InterProi | View protein in InterPro IPR003593 AAA+_ATPase IPR011527 ABC1_TM_dom IPR036640 ABC1_TM_sf IPR003439 ABC_transporter-like IPR017871 ABC_transporter_CS IPR027417 P-loop_NTPase |
Pfami | View protein in Pfam PF00664 ABC_membrane, 2 hits PF00005 ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382 AAA, 2 hits |
SUPFAMi | SSF52540 SSF52540, 2 hits SSF90123 SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929 ABC_TM1F, 2 hits PS00211 ABC_TRANSPORTER_1, 2 hits PS50893 ABC_TRANSPORTER_2, 2 hits |
ProtoNeti | Search... |
Entry informationi
Entry namei | YCFI_YEAST | |
Accessioni | P39109Primary (citable) accession number: P39109 Secondary accession number(s): D6VSB9, Q03905 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | May 30, 2000 | |
Last modified: | February 13, 2019 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD