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UniProtKB - P39098 (MA1A2_MOUSE)
Protein
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB
Gene
Man1a2
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
Catalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
Cofactori
Ca2+By similarity
Activity regulationi
Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 508 | Proton donorBy similarity | 1 | |
Metal bindingi | 619 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: GO_Central
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- glycoprotein metabolic process Source: MGI
- lung alveolus development Source: MGI
- N-glycan processing Source: GO_Central
- protein glycosylation Source: UniProtKB-UniPathway
- respiratory gaseous exchange by respiratory system Source: MGI
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.2.1.113, 3474 3.2.1.209, 3474 |
Reactomei | R-MMU-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC:3.2.1.113By similarity)Alternative name(s): Mannosidase alpha class 1A member 2 Processing alpha-1,2-mannosidase IB Short name: Alpha-1,2-mannosidase IB |
Gene namesi | Name:Man1a2 Synonyms:Man1b |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104676, Man1a2 |
VEuPathDBi | HostDB:ENSMUSG00000008763 |
Subcellular locationi
Golgi apparatus
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: MGI
- Golgi membrane Source: MGI
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 36 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 37 – 57 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 58 – 641 | LumenalSequence analysisAdd BLAST | 584 |
Keywords - Cellular componenti
Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000210313 | 2 – 641 | Mannosyl-oligosaccharide 1,2-alpha-mannosidase IBAdd BLAST | 640 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonineBy similarity | 1 | |
Disulfide bondi | 462 ↔ 494 | By similarity | ||
Glycosylationi | 631 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Acetylation, Disulfide bond, GlycoproteinProteomic databases
MaxQBi | P39098 |
PaxDbi | P39098 |
PeptideAtlasi | P39098 |
PRIDEi | P39098 |
ProteomicsDBi | 252705 [P39098-1] 252706 [P39098-2] |
PTM databases
GlyGeni | P39098, 1 site |
iPTMneti | P39098 |
PhosphoSitePlusi | P39098 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000008763, Expressed in spleen and 336 other tissues |
Genevisiblei | P39098, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 201303, 4 interactors |
STRINGi | 10090.ENSMUSP00000008907 |
Miscellaneous databases
RNActi | P39098, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000157498 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | P39098 |
OMAi | YSYLIHA |
OrthoDBi | 693882at2759 |
PhylomeDBi | P39098 |
TreeFami | TF313420 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P39098-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC
60 70 80 90 100
FGAFFFLPDS SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH
110 120 130 140 150
REEEERLRNK IRADHEKALE EAKEKLRKSR EEIRAEIQTE KNKVAQAMKT
160 170 180 190 200
KETRVLPPVP VPQRVGVSGG DPEDMEIKKK RDKIKEMMKH AWDNYRTYGW
210 220 230 240 250
GHNELRPIAR KGHSTNIFGS SQMGATIVDA LDTLYIMGLH DEFMDGQRWI
260 270 280 290 300
EENLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKTK AVQLAEKLLP
310 320 330 340 350
AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFVHLSYLT
360 370 380 390 400
GDLTYYNKVM HIRKLLQKME RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS
410 420 430 440 450
FYEYLLKAWL MSDKTDHEAR RMYDDAVEAI EKHLIKKSRG GLVFIGEWKN
460 470 480 490 500
GHLERKMGHL ACFAGGMFAL GADGSRKDKA GHYLELGAEI ARTCHESYDR
510 520 530 540 550
TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY LWRFTHDPRY
560 570 580 590 600
RQWGWEAALA IEKSCRVSGG FSGVKDVYAP TPVHDDVQQS FFLAETLKYL
610 620 630 640
YLLFSGDDLL PLDHWVFNTE AHPLPVLRLA NSTLSGNPAV R
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2A723 | A2A723_MOUSE | alpha-1,2-Mannosidase | Man1a2 | 246 | Annotation score: | ||
A0A0G2JGT9 | A0A0G2JGT9_MOUSE | Mannosyl-oligosaccharide 1,2-alpha-... | Man1a2 | 122 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 411 | M → T in AAB60438 (PubMed:8144579).Curated | 1 | |
Sequence conflicti | 468 | F → L in AAB60438 (PubMed:8144579).Curated | 1 | |
Sequence conflicti | 592 | F → S in AAB60438 (PubMed:8144579).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018615 | 259 – 285 | Missing in isoform 2. CuratedAdd BLAST | 27 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03458 mRNA Translation: AAB60439.1 U03457 mRNA Translation: AAB60438.1 AF078095 , AF078083, AF078084, AF078085, AF078086, AF078087, AF078088, AF078089, AF078090, AF078091, AF078092, AF078093, AF078094 Genomic DNA Translation: AAC34829.1 AK013515 mRNA Translation: BAB28892.3 AK153336 mRNA Translation: BAE31915.1 AL645760 Genomic DNA No translation available. BC049121 mRNA Translation: AAH49121.1 BC068192 mRNA Translation: AAH68192.1 |
CCDSi | CCDS17676.1 [P39098-1] |
PIRi | A54407 |
RefSeqi | NP_034893.1, NM_010763.2 [P39098-1] |
Genome annotation databases
Ensembli | ENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763 [P39098-1] |
GeneIDi | 17156 |
KEGGi | mmu:17156 |
UCSCi | uc008qqw.3, mouse [P39098-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03458 mRNA Translation: AAB60439.1 U03457 mRNA Translation: AAB60438.1 AF078095 , AF078083, AF078084, AF078085, AF078086, AF078087, AF078088, AF078089, AF078090, AF078091, AF078092, AF078093, AF078094 Genomic DNA Translation: AAC34829.1 AK013515 mRNA Translation: BAB28892.3 AK153336 mRNA Translation: BAE31915.1 AL645760 Genomic DNA No translation available. BC049121 mRNA Translation: AAH49121.1 BC068192 mRNA Translation: AAH68192.1 |
CCDSi | CCDS17676.1 [P39098-1] |
PIRi | A54407 |
RefSeqi | NP_034893.1, NM_010763.2 [P39098-1] |
3D structure databases
SMRi | P39098 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201303, 4 interactors |
STRINGi | 10090.ENSMUSP00000008907 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
PTM databases
GlyGeni | P39098, 1 site |
iPTMneti | P39098 |
PhosphoSitePlusi | P39098 |
Proteomic databases
MaxQBi | P39098 |
PaxDbi | P39098 |
PeptideAtlasi | P39098 |
PRIDEi | P39098 |
ProteomicsDBi | 252705 [P39098-1] 252706 [P39098-2] |
Protocols and materials databases
Antibodypediai | 33890, 95 antibodies from 25 providers |
DNASUi | 17156 |
Genome annotation databases
Ensembli | ENSMUST00000008907; ENSMUSP00000008907; ENSMUSG00000008763 [P39098-1] |
GeneIDi | 17156 |
KEGGi | mmu:17156 |
UCSCi | uc008qqw.3, mouse [P39098-1] |
Organism-specific databases
CTDi | 10905 |
MGIi | MGI:104676, Man1a2 |
VEuPathDBi | HostDB:ENSMUSG00000008763 |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000157498 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | P39098 |
OMAi | YSYLIHA |
OrthoDBi | 693882at2759 |
PhylomeDBi | P39098 |
TreeFami | TF313420 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 3.2.1.113, 3474 3.2.1.209, 3474 |
Reactomei | R-MMU-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
Miscellaneous databases
BioGRID-ORCSi | 17156, 8 hits in 64 CRISPR screens |
ChiTaRSi | Man1a2, mouse |
PROi | PR:P39098 |
RNActi | P39098, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000008763, Expressed in spleen and 336 other tissues |
Genevisiblei | P39098, MM |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MA1A2_MOUSE | |
Accessioni | P39098Primary (citable) accession number: P39098 Secondary accession number(s): Q5SUY6 Q9CUY9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families