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Protein

Palmitoyltransferase AKR1

Gene

AKR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Palmitoyltransferase specific for casein kinase 1. Palmitoylates isoforms YCK1 and YCK2 at both C-terminal cysteine residues, which is required for their proper plasma membrane localization. Required for constitutive endocytosis of a-factor receptor STE3 and both constitutive and pheromone-induced endocytosis of alpha-factor receptor STE2.5 Publications

Miscellaneous

Present with 4072 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei500S-palmitoyl cysteine intermediate1 Publication1

GO - Molecular functioni

  • G-protein beta/gamma-subunit complex binding Source: SGD
  • palmitoyltransferase activity Source: UniProtKB
  • protein-cysteine S-palmitoyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  • negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  • protein palmitoylation Source: UniProtKB
  • protein targeting to membrane Source: SGD
  • regulation of endocytosis Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-29834-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase AKR1 (EC:2.3.1.225)
Alternative name(s):
Ankyrin repeat-containing protein AKR1
Gene namesi
Name:AKR1
Ordered Locus Names:YDR264C
ORF Names:D9954.9, YD9230B.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR264C
SGDiS000002672 AKR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 321CytoplasmicAdd BLAST321
Transmembranei322 – 341HelicalSequence analysisAdd BLAST20
Topological domaini342 – 346Lumenal5
Transmembranei347 – 364HelicalSequence analysisAdd BLAST18
Topological domaini365 – 384CytoplasmicAdd BLAST20
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Topological domaini406 – 418LumenalAdd BLAST13
Transmembranei419 – 439HelicalSequence analysisAdd BLAST21
Topological domaini440 – 513CytoplasmicAdd BLAST74
Transmembranei514 – 534HelicalSequence analysisAdd BLAST21
Topological domaini535 – 570LumenalAdd BLAST36
Transmembranei571 – 591HelicalSequence analysisAdd BLAST21
Topological domaini592 – 764CytoplasmicAdd BLAST173

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi497 – 498DH → AA: Abolishes YCK2 palmitoylation. 1 Publication2
Mutagenesisi500C → A: Abolishes YCK2 palmitoylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002129341 – 764Palmitoyltransferase AKR1Add BLAST764

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei51PhosphoserineCombined sources1
Modified residuei57PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP39010
PaxDbiP39010
PRIDEiP39010

PTM databases

iPTMnetiP39010
SwissPalmiP39010

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
STE4P188513EBI-2421,EBI-7390

Protein-protein interaction databases

BioGridi32320, 188 interactors
DIPiDIP-1145N
IntActiP39010, 42 interactors
MINTiP39010
STRINGi4932.YDR264C

Structurei

3D structure databases

ProteinModelPortaliP39010
SMRiP39010
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati72 – 102ANK 1Add BLAST31
Repeati108 – 137ANK 2Add BLAST30
Repeati142 – 171ANK 3Add BLAST30
Repeati175 – 204ANK 4Add BLAST30
Repeati213 – 242ANK 5Add BLAST30
Repeati246 – 275ANK 6Add BLAST30
Domaini470 – 520DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000063074
HOGENOMiHOG000033883
InParanoidiP39010
KOiK20032
OMAiHLNFLFI
OrthoDBiEOG092C216R

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR001594 Palmitoyltrfase_DHHC
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF01529 DHHC, 1 hit
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 4 hits
PS50216 DHHC, 1 hit

Sequencei

Sequence statusi: Complete.

P39010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNELENVPR ASTLTNEEQT VDPSNNDSQE DISLGDSNEI TSLASLKAIR
60 70 80 90 100
SGNEEESGNE QVNHNDEAEE DPLLTRYHTA CQRGDLATVK EMIHGKLLEV
110 120 130 140 150
NNDGDSTEHI TGLHWASINN RLSVVDFLVS QGADVNARAG ALHATPLHWA
160 170 180 190 200
ARYGYVYIVD FLLKHGADPT MTDDQGFNLL HLSVNSSNIM LVLYVLFNVV
210 220 230 240 250
SKGLLDIDCR DPKGRTSLLW AAYQGDSLTV AELLKFGASI KIADTEGFTP
260 270 280 290 300
LHWGTVKGQP HVLKYLIQDG ADFFQKTDTG KDCFAIAQEM NTVYSLREAL
310 320 330 340 350
THSGFDYHGY PIKKWFKKSQ HAKLVTFITP FLFLGIAFAL FSHINPLFVI
360 370 380 390 400
IVLFLLAIAT NKGLNKFVLP SYGRMGVHNV TLLRSPLLSG VFFGTLLWVT
410 420 430 440 450
IVWFFKVMPR TFSDEQYTNI LMLVILVSVF YLFGQLVIMD PGCLPEETDH
460 470 480 490 500
ENVRQTISNL LEIGKFDTKN FCIETWIRKP LRSKFSPLNN AVVARFDHYC
510 520 530 540 550
PWIFNDVGLK NHKAFIFFIT LMESGIFTFL ALCLEYFDEL EDAHEDTSQK
560 570 580 590 600
NGKCFILGAS DLCSGLIYDR FVFLILLWAL LQSIWVASLI FVQAFQICKG
610 620 630 640 650
MTNTEFNVLM KESKSIGPDG LSFNENFNTT PEGFAPSIDP GEESNDTVLA
660 670 680 690 700
PVPGSTIRKP RTCFGVCYAV TGMDQWLAVI KETIGIKDST GHNVYSITSR
710 720 730 740 750
IPTNYGWKRN VKDFWLTSDI NAPLWRRILY PPSGSKALLN GIEVDYFKLY
760
KLPNKDVEQG NDMV
Length:764
Mass (Da):85,840
Last modified:February 1, 1995 - v1
Checksum:i9C9759F0140CE3F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31407 Genomic DNA Translation: AAC41676.1
U51030 Genomic DNA Translation: AAB64454.1
Z70202 Genomic DNA Translation: CAA94103.1
Z68290 Genomic DNA Translation: CAA92582.1
BK006938 Genomic DNA Translation: DAA12108.1
PIRiS48521
RefSeqiNP_010550.1, NM_001180572.1

Genome annotation databases

EnsemblFungiiYDR264C; YDR264C; YDR264C
GeneIDi851857
KEGGisce:YDR264C

Similar proteinsi

Entry informationi

Entry nameiAKR1_YEAST
AccessioniPrimary (citable) accession number: P39010
Secondary accession number(s): D6VSP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 18, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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