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Protein

DNA damage response protein kinase DUN1

Gene

DUN1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transducer of the DNA damage signal. Phosphorylates SML1 on serine residues. Cooperates with the PAN deadenylation complex in the regulation of RAD5 mRNA levels and cell survival in response to replicational stress.2 Publications

Miscellaneous

Present with 3480 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei229ATP1
Active sitei328Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi206 – 214ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cellular response to oxidative stress Source: GO_Central
  • DNA damage checkpoint Source: SGD
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: SGD
  • peptidyl-threonine phosphorylation Source: SGD
  • protein phosphorylation Source: SGD
  • replication fork protection Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processDNA damage
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29504-MONOMER
BRENDAi2.7.11.1 984

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage response protein kinase DUN1 (EC:2.7.11.1)
Gene namesi
Name:DUN1
Ordered Locus Names:YDL101C
ORF Names:D2370
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL101C
SGDiS000002259 DUN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi229K → R: Loss of kinase activity. 1 Publication1
Mutagenesisi328D → A: Loss of kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000859301 – 513DNA damage response protein kinase DUN1Add BLAST513

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei139PhosphoserineCombined sources1
Modified residuei380PhosphothreonineCombined sources1

Post-translational modificationi

Autophosphorylation increases in response to DNA damage.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39009
PaxDbiP39009
PRIDEiP39009

PTM databases

iPTMnetiP39009

Interactioni

Subunit structurei

Interacts with the PAB-dependent poly(A)-nuclease (PAN) complex regulatory subunit PAN3 via its forkhead-associated (FHA) domain.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31961, 485 interactors
DIPiDIP-1772N
IntActiP39009, 57 interactors
MINTiP39009
STRINGi4932.YDL101C

Structurei

Secondary structure

1513
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP39009
SMRiP39009
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39009

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 112FHAPROSITE-ProRule annotationAdd BLAST57
Domaini200 – 480Protein kinasePROSITE-ProRule annotationAdd BLAST281

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00840000129744
HOGENOMiHOG000233016
InParanoidiP39009
KOiK06641
OMAiSRAVDCW
OrthoDBiEOG092C0QJU

Family and domain databases

CDDicd00060 FHA, 1 hit
InterProiView protein in InterPro
IPR000253 FHA_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR008984 SMAD_FHA_dom_sf
PfamiView protein in Pfam
PF00498 FHA, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00240 FHA, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50006 FHA_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P39009-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLSTKREHS GDVTDSSFKR QQRSNKPSSE YTCLGHLVNL IPGKEQKVEI
60 70 80 90 100
TNRNVTTIGR SRSCDVILSE PDISTFHAEF HLLQMDVDNF QRNLINVIDK
110 120 130 140 150
SRNGTFINGN RLVKKDYILK NGDRIVFGKS CSFLFKYASS SSTDIENDDE
160 170 180 190 200
KVSSESRSYK NDDEVFKKPQ ISATSSQNAT TSAAIRKLNK TRPVSFFDKY
210 220 230 240 250
LLGKELGAGH YALVKEAKNK KTGQQVAVKI FHAQQNDDQK KNKQFREETN
260 270 280 290 300
ILMRVQHPNI VNLLDSFVEP ISKSQIQKYL VLEKIDDGEL FERIVRKTCL
310 320 330 340 350
RQDESKALFK QLLTGLKYLH EQNIIHRDIK PENILLNITR RENPSQVQLG
360 370 380 390 400
PWDEDEIDIQ VKIADFGLAK FTGEMQFTNT LCGTPSYVAP EVLTKKGYTS
410 420 430 440 450
KVDLWSAGVI LYVCLCGFPP FSDQLGPPSL KEQILQAKYA FYSPYWDKID
460 470 480 490 500
DSVLHLISNL LVLNPDERYN IDEALNHPWF NDIQQQSSVS LELQRLQITD
510
NKIPKTYSEL SCL
Length:513
Mass (Da):58,633
Last modified:February 1, 1995 - v1
Checksum:i4990F24F024702D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25548 Unassigned DNA Translation: AAA16324.1
X95644 Genomic DNA Translation: CAA64912.1
Z74149 Genomic DNA Translation: CAA98668.1
BK006938 Genomic DNA Translation: DAA11759.1
PIRiS43941
RefSeqiNP_010182.1, NM_001180160.1

Genome annotation databases

EnsemblFungiiYDL101C; YDL101C; YDL101C
GeneIDi851457
KEGGisce:YDL101C

Similar proteinsi

Entry informationi

Entry nameiDUN1_YEAST
AccessioniPrimary (citable) accession number: P39009
Secondary accession number(s): D6VRP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 12, 2018
This is version 184 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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