UniProtKB - P38907 (CENPN_YEAST)
Protein
Inner kinetochore subunit CHL4
Gene
CHL4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.1 Publication
Miscellaneous
Present with 606 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- structural molecule activity Source: SGD
GO - Biological processi
- cell division Source: UniProtKB-KW
- chromosome segregation Source: SGD
- establishment of meiotic sister chromatid cohesion Source: SGD
- establishment of mitotic sister chromatid cohesion Source: SGD
- kinetochore assembly Source: SGD
- maintenance of meiotic sister chromatid cohesion Source: SGD
- mitotic spindle assembly checkpoint Source: SGD
- protein localization to chromosome, centromeric region Source: SGD
Keywordsi
Biological process | Cell cycle, Cell division, Meiosis, Mitosis |
Names & Taxonomyi
Protein namesi | Recommended name: Inner kinetochore subunit CHL4CuratedAlternative name(s): CENP-N homolog1 Publication Chromosome loss protein 4 Chromosome transmission fidelity protein 17 Constitutive centromere-associated network protein CHL4Curated Minichromosome maintenance protein 17 |
Gene namesi | Name:CHL4 Synonyms:CTF17, MCM17 Ordered Locus Names:YDR254W ORF Names:YD9320A.04 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YDR254W |
SGDi | S000002662, CHL4 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- kinetochore 1 Publication
Note: Associated with kinetochores.
Keywords - Cellular componenti
Centromere, Chromosome, Kinetochore, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089646 | 1 – 458 | Inner kinetochore subunit CHL4Add BLAST | 458 |
Proteomic databases
MaxQBi | P38907 |
PaxDbi | P38907 |
Interactioni
Subunit structurei
Forms a heterodimer with IML3 (PubMed:12589047, PubMed:24075991). CHL4-IML3 is part of a larger constitutive centromere-associated network (CCAN) (also known as central kinetochore CTF19 complex in yeast), which is composed of at least AME1, CHL4, CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2, OKP1 and WIP1 (PubMed:12408861, PubMed:22561346).
Interacts with CTF3 and CTF19 (PubMed:12589047).
4 PublicationsProtein-protein interaction databases
BioGRIDi | 32304, 218 interactors |
ComplexPortali | CPX-1156, Central kinetochore CTF19 complex |
DIPi | DIP-5659N |
IntActi | P38907, 7 interactors |
MINTi | P38907 |
STRINGi | 4932.YDR254W |
Miscellaneous databases
RNActi | P38907, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P38907 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the CENP-N/CHL4 family.Curated
Phylogenomic databases
eggNOGi | ENOG502QVRZ, Eukaryota |
HOGENOMi | CLU_031572_0_0_1 |
InParanoidi | P38907 |
OMAi | PDVKAHY |
Family and domain databases
InterProi | View protein in InterPro IPR007902, Chl4/mis15/CENP-N |
Pfami | View protein in Pfam PF05238, CENP-N, 1 hit |
i Sequence
Sequence statusi: Complete.
P38907-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNELRLEDN YVPTSDTLVV FKQLMKLPVT VLYDLTLSWF AKFGGSFDGD
60 70 80 90 100
IYLLTETLDL LIEKGVRRNV IVNRILYVYW PDGLNVFQLA EIDCHLMISK
110 120 130 140 150
PEKFKWLPSK ALRGDGKPYV VKLQPAKFIE NLQTDLAKIY HCHVYMFKHP
160 170 180 190 200
SLPVLITRIQ LFDSNNLFLS TPNIGSINKE SLYNKLDKFQ GKPLISRRPY
210 220 230 240 250
YVAFPLNSPI IFHSVDKDIY ARLVLQSISR TISERETIIF KPVQKIPVKS
260 270 280 290 300
IHNIMTLLGP SRFAESMGPW ECYASANFER SPLHDYKKHQ GLTGKKVMVR
310 320 330 340 350
EFDDSFLNDD ENFYGKEEPE IRRLRLEKNM IKFKGSANGV MDQKYNDLKE
360 370 380 390 400
FNEHVHNIRN GKKNEDSGEP VYISRYSSLV PIEKVGFTLK NEINSRIITI
410 420 430 440 450
KLKFNGNDIF GGLHELCDKN LINIDKVPGW LAGENGSFSG TIMNGDFQRE
QVAKGGLL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S63181 Genomic DNA Translation: AAB27309.1 M92290 Unassigned DNA Translation: AAA16452.1 Z70202 Genomic DNA Translation: CAA94093.1 Z68329 Genomic DNA Translation: CAA92711.1 BK006938 Genomic DNA Translation: DAA12094.1 |
PIRi | S52594 |
RefSeqi | NP_010540.3, NM_001180562.3 |
Genome annotation databases
EnsemblFungii | YDR254W_mRNA; YDR254W; YDR254W |
GeneIDi | 851841 |
KEGGi | sce:YDR254W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S63181 Genomic DNA Translation: AAB27309.1 M92290 Unassigned DNA Translation: AAA16452.1 Z70202 Genomic DNA Translation: CAA94093.1 Z68329 Genomic DNA Translation: CAA92711.1 BK006938 Genomic DNA Translation: DAA12094.1 |
PIRi | S52594 |
RefSeqi | NP_010540.3, NM_001180562.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4JE3 | X-ray | 2.28 | B | 361-458 | [»] | |
6NUW | electron microscopy | 4.25 | E | 1-458 | [»] | |
6QLD | electron microscopy | 4.15 | N | 5-451 | [»] | |
6QLE | electron microscopy | 3.55 | N | 1-458 | [»] | |
6QLF | electron microscopy | 3.45 | N | 1-458 | [»] | |
SMRi | P38907 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32304, 218 interactors |
ComplexPortali | CPX-1156, Central kinetochore CTF19 complex |
DIPi | DIP-5659N |
IntActi | P38907, 7 interactors |
MINTi | P38907 |
STRINGi | 4932.YDR254W |
Proteomic databases
MaxQBi | P38907 |
PaxDbi | P38907 |
Genome annotation databases
EnsemblFungii | YDR254W_mRNA; YDR254W; YDR254W |
GeneIDi | 851841 |
KEGGi | sce:YDR254W |
Organism-specific databases
EuPathDBi | FungiDB:YDR254W |
SGDi | S000002662, CHL4 |
Phylogenomic databases
eggNOGi | ENOG502QVRZ, Eukaryota |
HOGENOMi | CLU_031572_0_0_1 |
InParanoidi | P38907 |
OMAi | PDVKAHY |
Miscellaneous databases
PROi | PR:P38907 |
RNActi | P38907, protein |
Family and domain databases
InterProi | View protein in InterPro IPR007902, Chl4/mis15/CENP-N |
Pfami | View protein in Pfam PF05238, CENP-N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CENPN_YEAST | |
Accessioni | P38907Primary (citable) accession number: P38907 Secondary accession number(s): D6VSN4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | February 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names