UniProtKB - P38879 (NACA_YEAST)
Protein
Nascent polypeptide-associated complex subunit alpha
Gene
EGD2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of the nascent polypeptide-associated complex (NAC), a dynamic component of the ribosomal exit tunnel, protecting the emerging polypeptides from interaction with other cytoplasmic proteins to ensure appropriate nascent protein targeting. The NAC complex also promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane and blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum. EGD2 may also be involved in transcription regulation.6 Publications
Miscellaneous
Present with 38016 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- phosphatidic acid binding Source: SGD
- phosphatidylinositol-3,5-bisphosphate binding Source: SGD
- phosphatidylinositol-3-phosphate binding Source: SGD
- phosphatidylinositol-4-phosphate binding Source: SGD
- unfolded protein binding Source: SGD
GO - Biological processi
- posttranslational protein targeting to endoplasmic reticulum membrane Source: SGD
- protein targeting to membrane Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Nascent polypeptide-associated complex subunit alphaShort name: NAC-alpha Alternative name(s): Alpha-NAC GAL4 DNA-binding enhancer protein 2 |
Gene namesi | Name:EGD2 Ordered Locus Names:YHR193C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YHR193C |
SGDi | S000001236, EGD2 |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: GO_Central
- nascent polypeptide-associated complex Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000135594 | 2 – 174 | Nascent polypeptide-associated complex subunit alphaAdd BLAST | 173 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 | |
Modified residuei | 93 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Ubiquitinated by the NOT4 E3 ligase and the UBC4 E2 ubiquitin conjugation enzyme.1 Publication
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P38879 |
PaxDbi | P38879 |
PRIDEi | P38879 |
TopDownProteomicsi | P38879 |
PTM databases
iPTMneti | P38879 |
Interactioni
Subunit structurei
Part of the nascent polypeptide-associated complex (NAC), consisting of EGD2 and either EGD1 or BTT1. NAC associates with ribosomes via EGD1 or BTT1, and with the CCR4-NOT complex.
Binary interactionsi
Hide detailsP38879
With | #Exp. | IntAct |
---|---|---|
EGD1 [Q02642] | 4 | EBI-6379,EBI-6371 |
UBC4 [P15731] | 2 | EBI-6379,EBI-19735 |
GO - Molecular functioni
- unfolded protein binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 36627, 291 interactors |
ComplexPortali | CPX-1306, Nascent polypeptide-associated complex, EGD1-EGD2 variant CPX-1307, Nascent polypeptide-associated complex, BTT1-EGD2 variant |
DIPi | DIP-2098N |
IntActi | P38879, 52 interactors |
MINTi | P38879 |
STRINGi | 4932.YHR193C |
Miscellaneous databases
RNActi | P38879, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 15 – 73 | NAC-A/BPROSITE-ProRule annotationAdd BLAST | 59 | |
Domaini | 135 – 174 | UBAAdd BLAST | 40 |
Domaini
The UBA domain is required for the stability of EGD1.
Sequence similaritiesi
Belongs to the NAC-alpha family.Curated
Phylogenomic databases
eggNOGi | KOG2239, Eukaryota |
GeneTreei | ENSGT00940000175567 |
HOGENOMi | CLU_057806_2_1_1 |
InParanoidi | P38879 |
OMAi | LVMCQAN |
Family and domain databases
Gene3Di | 2.20.70.30, 1 hit |
InterProi | View protein in InterPro IPR016641, EGD2/NACA IPR044034, NAC-like_UBA IPR038187, NAC_A/B_dom_sf IPR002715, Nas_poly-pep-assoc_cplx_dom IPR009060, UBA-like_sf |
PANTHERi | PTHR21713, PTHR21713, 1 hit |
Pfami | View protein in Pfam PF19026, HYPK_UBA, 1 hit PF01849, NAC, 1 hit |
PIRSFi | PIRSF015901, NAC_alpha, 1 hit |
SMARTi | View protein in SMART SM01407, NAC, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS51151, NAC_AB, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P38879-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAIPENANV TVLNKNEKKA RELIGKLGLK QIPGIIRVTF RKKDNQIYAI
60 70 80 90 100
EKPEVFRSAG GNYVVFGEAK VDNFTQKLAA AQQQAQASGI MPSNEDVATK
110 120 130 140 150
SPEDIQADMQ AAAEGSVNAA AEEDDEEGEV DAGDLNKDDI ELVVQQTNVS
160 170
KNQAIKALKA HNGDLVNAIM SLSK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17134 Genomic DNA Translation: AAA92080.1 U00030 Genomic DNA Translation: AAB68367.1 AY558288 Genomic DNA Translation: AAS56614.1 L12222 mRNA Translation: AAC15849.1 BK006934 Genomic DNA Translation: DAA06885.1 |
PIRi | S46689 |
RefSeqi | NP_012063.3, NM_001179324.3 |
Genome annotation databases
EnsemblFungii | YHR193C_mRNA; YHR193C; YHR193C |
GeneIDi | 856600 |
KEGGi | sce:YHR193C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U17134 Genomic DNA Translation: AAA92080.1 U00030 Genomic DNA Translation: AAB68367.1 AY558288 Genomic DNA Translation: AAS56614.1 L12222 mRNA Translation: AAC15849.1 BK006934 Genomic DNA Translation: DAA06885.1 |
PIRi | S46689 |
RefSeqi | NP_012063.3, NM_001179324.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36627, 291 interactors |
ComplexPortali | CPX-1306, Nascent polypeptide-associated complex, EGD1-EGD2 variant CPX-1307, Nascent polypeptide-associated complex, BTT1-EGD2 variant |
DIPi | DIP-2098N |
IntActi | P38879, 52 interactors |
MINTi | P38879 |
STRINGi | 4932.YHR193C |
PTM databases
iPTMneti | P38879 |
Proteomic databases
MaxQBi | P38879 |
PaxDbi | P38879 |
PRIDEi | P38879 |
TopDownProteomicsi | P38879 |
Genome annotation databases
EnsemblFungii | YHR193C_mRNA; YHR193C; YHR193C |
GeneIDi | 856600 |
KEGGi | sce:YHR193C |
Organism-specific databases
EuPathDBi | FungiDB:YHR193C |
SGDi | S000001236, EGD2 |
Phylogenomic databases
eggNOGi | KOG2239, Eukaryota |
GeneTreei | ENSGT00940000175567 |
HOGENOMi | CLU_057806_2_1_1 |
InParanoidi | P38879 |
OMAi | LVMCQAN |
Miscellaneous databases
ChiTaRSi | EGD2, yeast |
PROi | PR:P38879 |
RNActi | P38879, protein |
Family and domain databases
Gene3Di | 2.20.70.30, 1 hit |
InterProi | View protein in InterPro IPR016641, EGD2/NACA IPR044034, NAC-like_UBA IPR038187, NAC_A/B_dom_sf IPR002715, Nas_poly-pep-assoc_cplx_dom IPR009060, UBA-like_sf |
PANTHERi | PTHR21713, PTHR21713, 1 hit |
Pfami | View protein in Pfam PF19026, HYPK_UBA, 1 hit PF01849, NAC, 1 hit |
PIRSFi | PIRSF015901, NAC_alpha, 1 hit |
SMARTi | View protein in SMART SM01407, NAC, 1 hit |
SUPFAMi | SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS51151, NAC_AB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NACA_YEAST | |
Accessioni | P38879Primary (citable) accession number: P38879 Secondary accession number(s): D3DLE1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 188 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VIII
Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names