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Protein

DNA-dependent metalloprotease WSS1

Gene

WSS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Metalloendopeptidase that acts selectively on DNA-binding proteins. DNA is needed to bring the protease and substrates together to enable proteolysis. Involved in the repair of toxic DNA-protein cross-links (DPCs) such as covalently trapped topoisomerase 1 (TOP1) adducts on DNA lesions or DPCs induced by reactive compounds such as formaldehyde. Involved in DNA damage response and processing of stalled or collapsed replication forks by removing the covalently trapped TOP1 from chromatin. DPC proteolysis enables the repair of the lesions via downstream DNA repair pathways. May be recruited to DPCs via the SUMOylation of substrate proteins at damaged DNA sites.6 Publications

Caution

Was initially reported to be a SUMO-dependent isopeptidase (PubMed:20516210), but this activity could not be confirmed (PubMed:24998930).2 Publications

Activity regulationi

Inactivated by EDTA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115Zinc; catalyticPROSITE-ProRule annotation1
Active sitei116PROSITE-ProRule annotation1
Metal bindingi119Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi125Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processDNA damage
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31172-MONOMER

Protein family/group databases

MEROPSiM80.001

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-dependent metalloprotease WSS1 (EC:3.4.24.-)
Alternative name(s):
DNA damage response protein WSS1
SUMO-dependent isopeptidase WSS1
Weak suppressor of SMT3 protein 1
Gene namesi
Name:WSS1
Ordered Locus Names:YHR134W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR134W
SGDiS000001176 WSS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Accumulates DPCs and exhibits gross chromosomal rearrangements.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115H → A in wss1-pd; loss of function; when associated with A-119. 1 Publication1
Mutagenesisi116E → Q: Loss of function. 1 Publication1
Mutagenesisi119H → A in wss1-pd; loss of function; when associated with A-115. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002029191 – 269DNA-dependent metalloprotease WSS1Add BLAST269

Proteomic databases

PaxDbiP38838
PRIDEiP38838
TopDownProteomicsiP38838

Interactioni

Subunit structurei

Binds to DNA. Interacts with CDC48 and SMT3. Interacts with PSY2 and TOF1.3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi36567, 210 interactors
DIPiDIP-1600N
IntActiP38838, 4 interactors
MINTiP38838
STRINGi4932.YHR134W

Structurei

3D structure databases

ProteinModelPortaliP38838
SMRiP38838
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 221WLMPROSITE-ProRule annotationAdd BLAST202

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 208Required and sufficient for binding to DNAAdd BLAST48

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi152 – 160SHP box9
Motifi209 – 219VCP-interaction motif (VIM)Add BLAST11
Motifi247 – 254SUMO interaction motif 1 (SIM)8
Motifi266 – 269SUMO interaction motif 2 (SIM)4

Domaini

The SHP box and the VCP-interaction motif (VIM) are important both together for binding to CDC48. The 2 SUMO interaction motifs (SIM) are each important for binding to SMT3(SUMO).1 Publication

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000093863
InParanoidiP38838
KOiK22685
OMAiWCHSENA
OrthoDBiEOG092C3KVC

Family and domain databases

InterProiView protein in InterPro
IPR013536 WLM_dom
PfamiView protein in Pfam
PF08325 WLM, 1 hit
PROSITEiView protein in PROSITE
PS51397 WLM, 1 hit
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

P38838-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKTEGIKSPS AKYHDMAGSQ RIPHKNPHIQ KVAVLQSKPN KEDALNLIKE
60 70 80 90 100
IAHKVSYLMK ENHFKVTNLV EFYPRDQRLL GMNVNHGSKI MLRLRCSTDE
110 120 130 140 150
FQFLPMECIM GTMLHELTHN LFGPHDKKFY NKLDELIGRQ WVIEQRGLYD
160 170 180 190 200
TFLGNGQRLG GRANLRSNRY PMTGISTNTG IVRKRGKGVK LGSLHPEGIS
210 220 230 240 250
SIDRGNSPRE LAAFAAERRY RDDRWCGETK NNKDQIISDN ISSSLEVVIL
260
DDDDEVLPGD TLIEVIDLT
Length:269
Mass (Da):30,627
Last modified:February 1, 1995 - v1
Checksum:i09DC0854537F9D02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA Translation: AAB68404.1
BK006934 Genomic DNA Translation: DAA06827.1
PIRiS48978
RefSeqiNP_012002.1, NM_001179264.1

Genome annotation databases

EnsemblFungiiYHR134W; YHR134W; YHR134W
GeneIDi856536
KEGGisce:YHR134W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10398 Genomic DNA Translation: AAB68404.1
BK006934 Genomic DNA Translation: DAA06827.1
PIRiS48978
RefSeqiNP_012002.1, NM_001179264.1

3D structure databases

ProteinModelPortaliP38838
SMRiP38838
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36567, 210 interactors
DIPiDIP-1600N
IntActiP38838, 4 interactors
MINTiP38838
STRINGi4932.YHR134W

Protein family/group databases

MEROPSiM80.001

Proteomic databases

PaxDbiP38838
PRIDEiP38838
TopDownProteomicsiP38838

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR134W; YHR134W; YHR134W
GeneIDi856536
KEGGisce:YHR134W

Organism-specific databases

EuPathDBiFungiDB:YHR134W
SGDiS000001176 WSS1

Phylogenomic databases

HOGENOMiHOG000093863
InParanoidiP38838
KOiK22685
OMAiWCHSENA
OrthoDBiEOG092C3KVC

Enzyme and pathway databases

BioCyciYEAST:G3O-31172-MONOMER

Miscellaneous databases

PROiPR:P38838

Family and domain databases

InterProiView protein in InterPro
IPR013536 WLM_dom
PfamiView protein in Pfam
PF08325 WLM, 1 hit
PROSITEiView protein in PROSITE
PS51397 WLM, 1 hit
PS00142 ZINC_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWSS1_YEAST
AccessioniPrimary (citable) accession number: P38838
Secondary accession number(s): D3DL83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 12, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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