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Entry version 172 (17 Jun 2020)
Sequence version 1 (01 Feb 1995)
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Protein

Glutamine-dependent NAD(+) synthetase

Gene

QNS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine-dependent NAD(+) synthetase (QNS1)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (L-Gln route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei45Proton acceptor; for glutaminase activityBy similarity1
Active sitei114For glutaminase activityBy similarity1
Active sitei175Nucleophile; for glutaminase activityBy similarity1
Active sitei361By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi359 – 366ATPBy similarity8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLigase
LigandATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:MONOMER3O-845

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-196807 Nicotinate metabolism

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00253;UER00334

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Glutamine-dependent NAD(+) synthetase (EC:6.3.5.1)
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:QNS1
Ordered Locus Names:YHR074W
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome VIII

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YHR074W

Saccharomyces Genome Database

More...
SGDi
S000001116 QNS1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001522481 – 714Glutamine-dependent NAD(+) synthetaseAdd BLAST714

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P38795

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P38795

PRoteomics IDEntifications database

More...
PRIDEi
P38795

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P38795

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
36508, 172 interactors

Database of interacting proteins

More...
DIPi
DIP-5592N

Protein interaction database and analysis system

More...
IntActi
P38795, 3 interactors

Molecular INTeraction database

More...
MINTi
P38795

STRING: functional protein association networks

More...
STRINGi
4932.YHR074W

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P38795 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P38795

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini5 – 275CN hydrolasePROSITE-ProRule annotationAdd BLAST271

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni329 – 714LigaseAdd BLAST386

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the C-terminal section; belongs to the NAD synthetase family.Curated

Phylogenomic databases

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000010152

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_011884_2_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P38795

KEGG Orthology (KO)

More...
KOi
K01950

Identification of Orthologs from Complete Genome Data

More...
OMAi
LDFEGNM

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00553 NAD_synthase, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.620, 1 hit
3.60.110.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_02090 NadE_glutamine_dep, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR014445 Gln-dep_NAD_synthase
IPR022310 NAD/GMP_synthase
IPR003694 NAD_synthase
IPR014729 Rossmann-like_a/b/a_fold

The PANTHER Classification System

More...
PANTHERi
PTHR23090 PTHR23090, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00795 CN_hydrolase, 1 hit
PF02540 NAD_synthase, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF006630 NADS_GAT, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF56317 SSF56317, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00552 nadE, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P38795-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSHLITLATC NLNQWALDFE GNRDRILQSI KIAKERGARL RVGPELEITG
60 70 80 90 100
YGCLDHFLEN DVCLHSWEMY AQIIKNKETH GLILDIGMPV LHKNVRYNCR
110 120 130 140 150
LLSLDGEILF IRPKIWLAND GNYREMRFFT PWMKPGVVED FILPPEIQKV
160 170 180 190 200
TGQRLVPFGD AVINSLDTCI GTETCEELFT PQSPHIAMSL DGVEIMTNSS
210 220 230 240 250
GSHHELRKLN KRLDLILNAT KRCGGVYLYA NQRGCDGDRL YYDGCALIAI
260 270 280 290 300
NGTIVAQGSQ FSLDDVEVVT ATVDLEEVRS YRAAVMSRGL QASLAEIKFK
310 320 330 340 350
RIDIPVELAL MTSRFDPTVC PTKVREPFYH SPEEEIALGP ACWMWDYLRR
360 370 380 390 400
CNGTGFFLPL SGGIDSCATA MIVHSMCRLV TDAAQNGNEQ VIKDVRKITR
410 420 430 440 450
SGDDWIPDSP QDLASKIFHS CFMGTENSSK ETRNRAKDLS NAIGSYHVDL
460 470 480 490 500
KMDSLVSSVV SLFEVATGKK PIYKIFGGSQ IENLALQNIQ ARLRMVLSYL
510 520 530 540 550
FAQLLPWVRG IPNSGGLLVL GSANVDECLR GYLTKYDCSS ADINPIGGIS
560 570 580 590 600
KTDLKRFIAY ASKQYNMPIL NDFLNATPTA ELEPMTKDYV QSDEIDMGMT
610 620 630 640 650
YEELGVFGYL RKVEKCGPYS MFLKLLHQWS PKLTPRQISE KVKRFFFFYA
660 670 680 690 700
INRHKQTVLT PSYHAEQYSP EDNRFDLRPF LINPRFPWAS RKIDEVVEQC
710
EAHKGSTLDI MSID
Length:714
Mass (Da):80,686
Last modified:February 1, 1995 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iED8C723550F9E8A9
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U10556 Genomic DNA Translation: AAB68889.1
BK006934 Genomic DNA Translation: DAA06768.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S46811

NCBI Reference Sequences

More...
RefSeqi
NP_011941.1, NM_001179204.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YHR074W_mRNA; YHR074W; YHR074W

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
856473

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YHR074W

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA Translation: AAB68889.1
BK006934 Genomic DNA Translation: DAA06768.1
PIRiS46811
RefSeqiNP_011941.1, NM_001179204.1

3D structure databases

SMRiP38795
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi36508, 172 interactors
DIPiDIP-5592N
IntActiP38795, 3 interactors
MINTiP38795
STRINGi4932.YHR074W

PTM databases

iPTMnetiP38795

Proteomic databases

MaxQBiP38795
PaxDbiP38795
PRIDEiP38795

Genome annotation databases

EnsemblFungiiYHR074W_mRNA; YHR074W; YHR074W
GeneIDi856473
KEGGisce:YHR074W

Organism-specific databases

EuPathDBiFungiDB:YHR074W
SGDiS000001116 QNS1

Phylogenomic databases

GeneTreeiENSGT00390000010152
HOGENOMiCLU_011884_2_0_1
InParanoidiP38795
KOiK01950
OMAiLDFEGNM

Enzyme and pathway databases

UniPathwayiUPA00253;UER00334
BioCyciYEAST:MONOMER3O-845
ReactomeiR-SCE-196807 Nicotinate metabolism

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P38795
RNActiP38795 protein

Family and domain databases

CDDicd00553 NAD_synthase, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.110.10, 1 hit
HAMAPiMF_02090 NadE_glutamine_dep, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
IPR014445 Gln-dep_NAD_synthase
IPR022310 NAD/GMP_synthase
IPR003694 NAD_synthase
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR23090 PTHR23090, 1 hit
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
PF02540 NAD_synthase, 1 hit
PIRSFiPIRSF006630 NADS_GAT, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
TIGRFAMsiTIGR00552 nadE, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNADE_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P38795
Secondary accession number(s): D3DL24
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 17, 2020
This is version 172 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names
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