UniProtKB - P38649 (BGAT_MOUSE)
Protein
Histo-blood group ABO system transferase
Gene
Abo
Organism
Mus musculus (Mouse)
Status
Functioni
Catalytic activityi
- an α-L-fucosyl-(1→2)-β-D-galactosyl derivative + UDP-N-acetyl-α-D-galactosamine = an N-acetyl-α-D-galactosaminyl-(1→3)-[α-L-fucosyl-(1→2)]-β-D-galactosyl derivative + H+ + UDPBy similarityEC:2.4.1.40By similarity
- an α-L-fucosyl-(1→2)-β-D-galactosyl derivative + UDP-α-D-galactose = an α-D-galactosyl-(1→3)-[α-L-fucosyl-(1→2)]-β-D-galactosyl derivative + H+ + UDPBy similarityEC:2.4.1.37By similarity
Cofactori
Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 105 | UDP-N-acetyl-galactosamineBy similarity | 1 | |
Metal bindingi | 190 | ManganeseBy similarity | 1 | |
Metal bindingi | 192 | ManganeseBy similarity | 1 | |
Binding sitei | 212 | Glycoprotein fucosyl-galactosyl groupBy similarity | 1 | |
Binding sitei | 224 | Glycoprotein fucosyl-galactosyl groupBy similarity | 1 | |
Active sitei | 282 | NucleophileBy similarity | 1 | |
Binding sitei | 282 | Glycoprotein fucosyl-galactosyl groupBy similarity | 1 | |
Binding sitei | 305 | Glycoprotein fucosyl-galactosyl groupBy similarity | 1 |
GO - Molecular functioni
- alpha-1,3-galactosyltransferase activity Source: MGI
- antigen binding Source: MGI
- fucosylgalactoside 3-alpha-galactosyltransferase activity Source: MGI
- glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity Source: MGI
- manganese ion binding Source: MGI
- nucleotide binding Source: MGI
- transferase activity, transferring glycosyl groups Source: GO_Central
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- lipid glycosylation Source: GO_Central
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BRENDAi | 2.4.1.37, 3477 2.4.1.40, 3477 |
Reactomei | R-MMU-9033807, ABO blood group biosynthesis |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT6, Glycosyltransferase Family 6 |
Names & Taxonomyi
Protein namesi | Recommended name: Histo-blood group ABO system transferaseAlternative name(s): Cis-AB transferase Fucosylglycoprotein 3-alpha-galactosyltransferase Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40By similarity) Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37By similarity) Histo-blood group A transferase Short name: A transferase Histo-blood group B transferase Short name: B transferase NAGAT |
Gene namesi | Name:Abo |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2135738, Abo |
Subcellular locationi
Extracellular region or secreted
Golgi apparatus
Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid (By similarity).By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: MGI
- Golgi cisterna membrane Source: UniProtKB-SubCell
Other locations
- cytoplasmic vesicle Source: MGI
- integral component of membrane Source: UniProtKB-KW
- vesicle Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 13 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 14 – 34 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 35 – 332 | LumenalSequence analysisAdd BLAST | 298 |
Keywords - Cellular componenti
Golgi apparatus, Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000157293 | 1 – 332 | Histo-blood group ABO system transferaseAdd BLAST | 332 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 92 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | P38649 |
PRIDEi | P38649 |
PTM databases
GlyGeni | P38649, 1 site |
iPTMneti | P38649 |
PhosphoSitePlusi | P38649 |
Expressioni
Tissue specificityi
Submaxillary glands (at protein level).1 Publication
Gene expression databases
Bgeei | ENSMUSG00000015787, Expressed in prostate gland ventral lobe and 57 other tissues |
ExpressionAtlasi | P38649, baseline and differential |
Genevisiblei | P38649, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000099964 |
Miscellaneous databases
RNActi | P38649, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 100 – 102 | UDP-N-acetyl-galactosamine bindingBy similarity | 3 | |
Regioni | 190 – 192 | UDP-N-acetyl-galactosamine bindingBy similarity | 3 |
Domaini
The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
Sequence similaritiesi
Belongs to the glycosyltransferase 6 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QQAJ, Eukaryota |
GeneTreei | ENSGT00950000182858 |
HOGENOMi | CLU_062445_0_1_1 |
InParanoidi | P38649 |
OrthoDBi | 1204439at2759 |
PhylomeDBi | P38649 |
TreeFami | TF330991 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR005076, Glyco_trans_6 IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR10462, PTHR10462, 1 hit |
Pfami | View protein in Pfam PF03414, Glyco_transf_6, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P38649-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLRGRPKCN FLHLGILPFA VFVLVFFGYL FLSFRSQNLG HPGAVTRNAY
60 70 80 90 100
LQPRVLKPTR KDVLVLTPWL APIIWEGTFN IDILNEQFRI RNTTIGLTVF
110 120 130 140 150
AIKKYVVFLK LFLETAEQHF MVGHKVIYYV FTDRPADVPQ VILGAGRQLV
160 170 180 190 200
VLTVRNYTRW QDVSMHRMEM ISHFSERRFL REVDYLVCAD ADMKFSDHVG
210 220 230 240 250
VEILSTFFGT LHPGFYSSSR EAFTYERRPQ SQAYIPWDRG DFYYGGAFFG
260 270 280 290 300
GSVLEVYHLT KACHEAMMED KANGIEPVWH DESYLNKYLL YHKPTKVLSP
310 320 330
EYLWDQQLLG WPSIMKKLRY VAVPKDHQAI RN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketZ4YLB0 | Z4YLB0_MOUSE | Histo-blood group ABO system transf... | Abo | 334 | Annotation score: |
Sequence cautioni
The sequence CAM23695 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 77 | G → R in BAC29005 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 109 | L → I in BAC29005 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 160 | W → C in BAC28473 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB041038 Genomic DNA Translation: BAB20559.1 AB041039 mRNA Translation: BAB20560.1 AK033786 mRNA Translation: BAC28473.1 AK035261 mRNA Translation: BAC29005.1 AL773563 Genomic DNA Translation: CAM23695.1 Sequence problems. AL773563 Genomic DNA Translation: CAM23696.1 CH466542 Genomic DNA Translation: EDL08332.1 BC116759 mRNA Translation: AAI16760.1 BC116761 mRNA Translation: AAI16762.1 |
CCDSi | CCDS15811.1 |
RefSeqi | NP_001277373.1, NM_001290444.1 NP_109643.3, NM_030718.5 |
Genome annotation databases
Ensembli | ENSMUST00000102900; ENSMUSP00000099964; ENSMUSG00000015787 |
GeneIDi | 80908 |
KEGGi | mmu:80908 |
UCSCi | uc008iwb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB041038 Genomic DNA Translation: BAB20559.1 AB041039 mRNA Translation: BAB20560.1 AK033786 mRNA Translation: BAC28473.1 AK035261 mRNA Translation: BAC29005.1 AL773563 Genomic DNA Translation: CAM23695.1 Sequence problems. AL773563 Genomic DNA Translation: CAM23696.1 CH466542 Genomic DNA Translation: EDL08332.1 BC116759 mRNA Translation: AAI16760.1 BC116761 mRNA Translation: AAI16762.1 |
CCDSi | CCDS15811.1 |
RefSeqi | NP_001277373.1, NM_001290444.1 NP_109643.3, NM_030718.5 |
3D structure databases
SMRi | P38649 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000099964 |
Protein family/group databases
CAZyi | GT6, Glycosyltransferase Family 6 |
PTM databases
GlyGeni | P38649, 1 site |
iPTMneti | P38649 |
PhosphoSitePlusi | P38649 |
Proteomic databases
PaxDbi | P38649 |
PRIDEi | P38649 |
Protocols and materials databases
Antibodypediai | 80247, 787 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000102900; ENSMUSP00000099964; ENSMUSG00000015787 |
GeneIDi | 80908 |
KEGGi | mmu:80908 |
UCSCi | uc008iwb.2, mouse |
Organism-specific databases
CTDi | 28 |
MGIi | MGI:2135738, Abo |
Phylogenomic databases
eggNOGi | ENOG502QQAJ, Eukaryota |
GeneTreei | ENSGT00950000182858 |
HOGENOMi | CLU_062445_0_1_1 |
InParanoidi | P38649 |
OrthoDBi | 1204439at2759 |
PhylomeDBi | P38649 |
TreeFami | TF330991 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 2.4.1.37, 3477 2.4.1.40, 3477 |
Reactomei | R-MMU-9033807, ABO blood group biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 80908, 0 hits in 17 CRISPR screens |
ChiTaRSi | Abo, mouse |
PROi | PR:P38649 |
RNActi | P38649, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015787, Expressed in prostate gland ventral lobe and 57 other tissues |
ExpressionAtlasi | P38649, baseline and differential |
Genevisiblei | P38649, MM |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR005076, Glyco_trans_6 IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR10462, PTHR10462, 1 hit |
Pfami | View protein in Pfam PF03414, Glyco_transf_6, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BGAT_MOUSE | |
Accessioni | P38649Primary (citable) accession number: P38649 Secondary accession number(s): A2AL98 Q9EQW3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1995 |
Last sequence update: | December 16, 2008 | |
Last modified: | February 10, 2021 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families