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Protein

Replication factor C subunit 1

Gene

RFC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ATP-dependent clamp loader RFC complex for the POL30/PCNA homotrimer DNA clamp. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Replication factor C (RFC or activator 1) complex acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment.

Miscellaneous

Present with 2360 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei299ATP; via carbonyl oxygen1
Binding sitei311ATP; via amide nitrogen and carbonyl oxygen1
Binding sitei456ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi353 – 361ATP9

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • DNA repair Source: SGD
  • leading strand elongation Source: SGD
  • mismatch repair Source: SGD
  • mitotic cell cycle Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33719-MONOMER
ReactomeiR-SCE-110312 Translesion synthesis by REV1
R-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-110320 Translesion Synthesis by POLH
R-SCE-174411 Polymerase switching on the C-strand of the telomere
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5655862 Translesion synthesis by POLK
R-SCE-5656121 Translesion synthesis by POLI
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-69091 Polymerase switching

Names & Taxonomyi

Protein namesi
Recommended name:
Replication factor C subunit 1
Short name:
Replication factor C1
Alternative name(s):
Activator 1 95 kDa subunit
Cell division control protein 44
Gene namesi
Name:RFC1
Synonyms:CDC44
Ordered Locus Names:YOR217W
ORF Names:YOR50-7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR217W
SGDiS000005743 RFC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi427D → H in cs mutant CDC44-2; causes cell cycle arrest. 1 Publication1
Mutagenesisi436G → R in cs mutant CDC44-3/4; causes cell cycle arrest. 1 Publication1
Mutagenesisi512G → A in cs mutant CDC44-9; no effect. 1 Publication1
Mutagenesisi513D → N in cs mutants CDC44-1/5/8 and CDC44-9; causes cell cycle arrest. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001217761 – 861Replication factor C subunit 1Add BLAST861

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38PhosphothreonineCombined sources1
Modified residuei40PhosphoserineCombined sources1
Modified residuei63PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38630
PaxDbiP38630
PRIDEiP38630

PTM databases

iPTMnetiP38630

Interactioni

Subunit structurei

Replication factor C (RFC) is a heteropentamer of subunits RFC1, RFC2, RFC3, RFC4 and RFC5 and forms a complex with POL30/PCNA in the presence of ATP. Interacts with ECO1 and POL30/PCNA.5 Publications

Protein-protein interaction databases

BioGridi34612, 275 interactors
ComplexPortaliCPX-545 DNA replication factor C complex
DIPiDIP-2527N
ELMiP38630
IntActiP38630, 16 interactors
MINTiP38630
STRINGi4932.YOR217W

Structurei

Secondary structure

1861
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP38630
SMRiP38630
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38630

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 243BRCTPROSITE-ProRule annotationAdd BLAST91

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi830 – 834Nuclear localization signalSequence analysis5
Motifi855 – 860Nuclear localization signalSequence analysis6

Sequence similaritiesi

Belongs to the activator 1 large subunit family.Curated

Phylogenomic databases

GeneTreeiENSGT00730000111066
HOGENOMiHOG000164552
InParanoidiP38630
KOiK10754
OMAiPKMKPFD
OrthoDBiEOG092C1OUP

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR013725 DNA_replication_fac_RFC1_C
IPR027417 P-loop_NTPase
IPR012178 RFC1
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF00533 BRCT, 1 hit
PF08519 RFC1, 1 hit
PIRSFiPIRSF036578 RFC1, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00292 BRCT, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52113 SSF52113, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit

Sequencei

Sequence statusi: Complete.

P38630-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVNISDFFGK NKKSVRSSTS RPTRQVGSSK PEVIDLDTES DQESTNKTPK
60 70 80 90 100
KMPVSNVIDV SETPEGEKKL PLPAKRKASS PTVKPASSKK TKPSSKSSDS
110 120 130 140 150
ASNITAQDVL DKIPSLDLSN VHVKENAKFD FKSANSNADP DEIVSEIGSF
160 170 180 190 200
PEGKPNCLLG LTIVFTGVLP TLERGASEAL AKRYGARVTK SISSKTSVVV
210 220 230 240 250
LGDEAGPKKL EKIKQLKIKA IDEEGFKQLI AGMPAEGGDG EAAEKARRKL
260 270 280 290 300
EEQHNIATKE AELLVKKEEE RSKKLAATRV SGGHLERDNV VREEDKLWTV
310 320 330 340 350
KYAPTNLQQV CGNKGSVMKL KNWLANWENS KKNSFKHAGK DGSGVFRAAM
360 370 380 390 400
LYGPPGIGKT TAAHLVAQEL GYDILEQNAS DVRSKTLLNA GVKNALDNMS
410 420 430 440 450
VVGYFKHNEE AQNLNGKHFV IIMDEVDGMS GGDRGGVGQL AQFCRKTSTP
460 470 480 490 500
LILICNERNL PKMRPFDRVC LDIQFRRPDA NSIKSRLMTI AIREKFKLDP
510 520 530 540 550
NVIDRLIQTT RGDIRQVINL LSTISTTTKT INHENINEIS KAWEKNIALK
560 570 580 590 600
PFDIAHKMLD GQIYSDIGSR NFTLNDKIAL YFDDFDFTPL MIQENYLSTR
610 620 630 640 650
PSVLKPGQSH LEAVAEAANC ISLGDIVEKK IRSSEQLWSL LPLHAVLSSV
660 670 680 690 700
YPASKVAGHM AGRINFTAWL GQNSKSAKYY RLLQEIHYHT RLGTSTDKIG
710 720 730 740 750
LRLDYLPTFR KRLLDPFLKQ GADAISSVIE VMDDYYLTKE DWDSIMEFFV
760 770 780 790 800
GPDVTTAIIK KIPATVKSGF TRKYNSMTHP VAIYRTGSTI GGGGVGTSTS
810 820 830 840 850
TPDFEDVVDA DDNPVPADDE ETQDSSTDLK KDKLIKQKAK PTKRKTATSK
860
PGGSKKRKTK A
Length:861
Mass (Da):94,903
Last modified:October 1, 1994 - v1
Checksum:iA7D9208D66DD9A98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03102 Unassigned DNA Translation: AAC48916.1
U26027 Genomic DNA Translation: AAC49060.1
X92441 Genomic DNA Translation: CAA63180.1
Z75125 Genomic DNA Translation: CAA99434.1
BK006948 Genomic DNA Translation: DAA10989.1
PIRiS44763
RefSeqiNP_014860.1, NM_001183636.1

Genome annotation databases

EnsemblFungiiYOR217W; YOR217W; YOR217W
GeneIDi854392
KEGGisce:YOR217W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03102 Unassigned DNA Translation: AAC48916.1
U26027 Genomic DNA Translation: AAC49060.1
X92441 Genomic DNA Translation: CAA63180.1
Z75125 Genomic DNA Translation: CAA99434.1
BK006948 Genomic DNA Translation: DAA10989.1
PIRiS44763
RefSeqiNP_014860.1, NM_001183636.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SXJX-ray2.85A295-785[»]
ProteinModelPortaliP38630
SMRiP38630
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34612, 275 interactors
ComplexPortaliCPX-545 DNA replication factor C complex
DIPiDIP-2527N
ELMiP38630
IntActiP38630, 16 interactors
MINTiP38630
STRINGi4932.YOR217W

PTM databases

iPTMnetiP38630

Proteomic databases

MaxQBiP38630
PaxDbiP38630
PRIDEiP38630

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR217W; YOR217W; YOR217W
GeneIDi854392
KEGGisce:YOR217W

Organism-specific databases

EuPathDBiFungiDB:YOR217W
SGDiS000005743 RFC1

Phylogenomic databases

GeneTreeiENSGT00730000111066
HOGENOMiHOG000164552
InParanoidiP38630
KOiK10754
OMAiPKMKPFD
OrthoDBiEOG092C1OUP

Enzyme and pathway databases

BioCyciYEAST:G3O-33719-MONOMER
ReactomeiR-SCE-110312 Translesion synthesis by REV1
R-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-110320 Translesion Synthesis by POLH
R-SCE-174411 Polymerase switching on the C-strand of the telomere
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5655862 Translesion synthesis by POLK
R-SCE-5656121 Translesion synthesis by POLI
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-69091 Polymerase switching

Miscellaneous databases

EvolutionaryTraceiP38630
PROiPR:P38630

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR013725 DNA_replication_fac_RFC1_C
IPR027417 P-loop_NTPase
IPR012178 RFC1
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF00533 BRCT, 1 hit
PF08519 RFC1, 1 hit
PIRSFiPIRSF036578 RFC1, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00292 BRCT, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52113 SSF52113, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRFC1_YEAST
AccessioniPrimary (citable) accession number: P38630
Secondary accession number(s): D6W2S3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 12, 2018
This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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Main funding by: National Institutes of Health

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