RFC1

Functionⁱ

Component of the ATP-dependent clamp loader RFC complex for the POL30/PCNA homotrimer DNA clamp. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Replication factor C (RFC or activator 1) complex acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment.

Miscellaneous

Present with 2360 molecules/cell in log phase SD medium.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	299	ATP; via carbonyl oxygen	1
Binding siteⁱ	311	ATP; via amide nitrogen and carbonyl oxygen	1
Binding siteⁱ	456	ATP	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	353 – 361	ATP		9

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
DNA clamp loader activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell division Source: UniProtKB-KW
DNA repair
Source: SGD
Inferred from mutant phenotypeⁱ
- 8770585
leading strand elongation
Source: SGD
Inferred from direct assayⁱ
- 1346062
mismatch repair
Source: SGD
Inferred from genetic interactionⁱ
- 9504910
mitotic cell cycle
Source: SGD
Inferred from mutant phenotypeⁱ
- 6749598
- 6749599

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Cell cycle, Cell division, DNA replication
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-33719-MONOMER
Reactomeⁱ	R-SCE-110312 Translesion synthesis by REV1 R-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex R-SCE-110320 Translesion Synthesis by POLH R-SCE-174411 Polymerase switching on the C-strand of the telomere R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair R-SCE-5655862 Translesion synthesis by POLK R-SCE-5656121 Translesion synthesis by POLI R-SCE-5656169 Termination of translesion DNA synthesis R-SCE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-SCE-5696400 Dual Incision in GG-NER R-SCE-6782135 Dual incision in TC-NER R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-69091 Polymerase switching

BioCycⁱ

YEAST:G3O-33719-MONOMER

Reactomeⁱ

R-SCE-110312 Translesion synthesis by REV1
R-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-110320 Translesion Synthesis by POLH
R-SCE-174411 Polymerase switching on the C-strand of the telomere
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5655862 Translesion synthesis by POLK
R-SCE-5656121 Translesion synthesis by POLI
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-69091 Polymerase switching

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Replication factor C subunit 1 Short name: Replication factor C1 Alternative name(s): Activator 1 95 kDa subunit Cell division control protein 44
Gene namesⁱ	Name:RFC1 Synonyms:CDC44 Ordered Locus Names:YOR217W ORF Names:YOR50-7
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome XV

Organism-specific databases

EuPathDBⁱ	FungiDB:YOR217W
Saccharomyces Genome Database More...SGDⁱ	S000005743 RFC1

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	427	D → H in cs mutant CDC44-2; causes cell cycle arrest. 1 Publication	1
Mutagenesisⁱ	436	G → R in cs mutant CDC44-3/4; causes cell cycle arrest. 1 Publication	1
Mutagenesisⁱ	512	G → A in cs mutant CDC44-9; no effect. 1 Publication	1
Mutagenesisⁱ	513	D → N in cs mutants CDC44-1/5/8 and CDC44-9; causes cell cycle arrest. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000121776	1 – 861	Replication factor C subunit 1Add BLAST		861

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	38	PhosphothreonineCombined sources	1
Modified residueⁱ	40	PhosphoserineCombined sources	1
Modified residueⁱ	63	PhosphothreonineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P38630
PaxDbⁱ	P38630
PRIDEⁱ	P38630

PTM databases

iPTMnetⁱ	P38630

iPTMnetⁱ

P38630

Interactionⁱ

Subunit structureⁱ

Replication factor C (RFC) is a heteropentamer of subunits RFC1, RFC2, RFC3, RFC4 and RFC5 and forms a complex with POL30/PCNA in the presence of ATP. Interacts with ECO1 and POL30/PCNA.5 Publications

Protein-protein interaction databases

BioGridⁱ	34612, 275 interactors
ComplexPortalⁱ	CPX-545 DNA replication factor C complex
Database of interacting proteins More...DIPⁱ	DIP-2527N
ELMⁱ	P38630
IntActⁱ	P38630, 16 interactors
Molecular INTeraction database More...MINTⁱ	P38630
STRINGⁱ	4932.YOR217W

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	298 – 301	Combined sources	4
Helixⁱ	307 – 309	Combined sources	3
Helixⁱ	314 – 325	Combined sources	12
Helixⁱ	327 – 332	Combined sources	6
Turnⁱ	333 – 335	Combined sources	3
Beta strandⁱ	347 – 352	Combined sources	6
Helixⁱ	359 – 369	Combined sources	11
Beta strandⁱ	373 – 377	Combined sources	5
Helixⁱ	385 – 390	Combined sources	6
Helixⁱ	392 – 395	Combined sources	4
Turnⁱ	402 – 406	Combined sources	5
Beta strandⁱ	418 – 423	Combined sources	6
Helixⁱ	426 – 428	Combined sources	3
Helixⁱ	436 – 446	Combined sources	11
Beta strandⁱ	451 – 456	Combined sources	6
Helixⁱ	464 – 466	Combined sources	3
Turnⁱ	467 – 469	Combined sources	3
Beta strandⁱ	470 – 474	Combined sources	5
Helixⁱ	480 – 493	Combined sources	14
Helixⁱ	502 – 509	Combined sources	8
Turnⁱ	510 – 512	Combined sources	3
Helixⁱ	514 – 521	Combined sources	8
Helixⁱ	523 – 527	Combined sources	5
Helixⁱ	535 – 544	Combined sources	10
Turnⁱ	545 – 548	Combined sources	4
Helixⁱ	550 – 558	Combined sources	9
Helixⁱ	561 – 563	Combined sources	3
Helixⁱ	568 – 570	Combined sources	3
Helixⁱ	574 – 581	Combined sources	8
Turnⁱ	585 – 587	Combined sources	3
Helixⁱ	588 – 595	Combined sources	8
Beta strandⁱ	596 – 602	Combined sources	7
Helixⁱ	610 – 631	Combined sources	22
Helixⁱ	638 – 640	Combined sources	3
Helixⁱ	641 – 648	Combined sources	8
Helixⁱ	650 – 654	Combined sources	5
Helixⁱ	669 – 687	Combined sources	19
Turnⁱ	688 – 692	Combined sources	5
Helixⁱ	699 – 703	Combined sources	5
Turnⁱ	704 – 706	Combined sources	3
Helixⁱ	707 – 714	Combined sources	8
Helixⁱ	725 – 730	Combined sources	6
Turnⁱ	731 – 733	Combined sources	3
Helixⁱ	737 – 746	Combined sources	10

Show more details

3D structure databases

ProteinModelPortalⁱ	P38630
SMRⁱ	P38630
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P38630

EvolutionaryTraceⁱ

P38630

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	153 – 243	BRCTPROSITE-ProRule annotationAdd BLAST		91

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	830 – 834	Nuclear localization signalSequence analysis		5
Motifⁱ	855 – 860	Nuclear localization signalSequence analysis		6

Sequence similaritiesⁱ

Belongs to the activator 1 large subunit family.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00730000111066
HOGENOMⁱ	HOG000164552
InParanoidⁱ	P38630
KEGG Orthology (KO) More...KOⁱ	K10754
OMAⁱ	PKMKPFD
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C1OUP

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00027 BRCT, 1 hit
Gene3Dⁱ	3.40.50.10190, 1 hit
InterProⁱ	View protein in InterPro IPR003593 AAA+_ATPase IPR003959 ATPase_AAA_core IPR001357 BRCT_dom IPR036420 BRCT_dom_sf IPR008921 DNA_pol3_clamp-load_cplx_C IPR013725 DNA_replication_fac_RFC1_C IPR027417 P-loop_NTPase IPR012178 RFC1
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00004 AAA, 1 hit PF00533 BRCT, 1 hit PF08519 RFC1, 1 hit
PIRSFⁱ	PIRSF036578 RFC1, 1 hit
SMARTⁱ	View protein in SMART SM00382 AAA, 1 hit SM00292 BRCT, 1 hit
SUPFAMⁱ	SSF48019 SSF48019, 1 hit SSF52113 SSF52113, 1 hit SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS50172 BRCT, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P38630-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVNISDFFGK NKKSVRSSTS RPTRQVGSSK PEVIDLDTES DQESTNKTPK 
        60         70         80         90        100
KMPVSNVIDV SETPEGEKKL PLPAKRKASS PTVKPASSKK TKPSSKSSDS 
       110        120        130        140        150
ASNITAQDVL DKIPSLDLSN VHVKENAKFD FKSANSNADP DEIVSEIGSF 
       160        170        180        190        200
PEGKPNCLLG LTIVFTGVLP TLERGASEAL AKRYGARVTK SISSKTSVVV 
       210        220        230        240        250
LGDEAGPKKL EKIKQLKIKA IDEEGFKQLI AGMPAEGGDG EAAEKARRKL 
       260        270        280        290        300
EEQHNIATKE AELLVKKEEE RSKKLAATRV SGGHLERDNV VREEDKLWTV 
       310        320        330        340        350
KYAPTNLQQV CGNKGSVMKL KNWLANWENS KKNSFKHAGK DGSGVFRAAM 
       360        370        380        390        400
LYGPPGIGKT TAAHLVAQEL GYDILEQNAS DVRSKTLLNA GVKNALDNMS 
       410        420        430        440        450
VVGYFKHNEE AQNLNGKHFV IIMDEVDGMS GGDRGGVGQL AQFCRKTSTP 
       460        470        480        490        500
LILICNERNL PKMRPFDRVC LDIQFRRPDA NSIKSRLMTI AIREKFKLDP 
       510        520        530        540        550
NVIDRLIQTT RGDIRQVINL LSTISTTTKT INHENINEIS KAWEKNIALK 
       560        570        580        590        600
PFDIAHKMLD GQIYSDIGSR NFTLNDKIAL YFDDFDFTPL MIQENYLSTR 
       610        620        630        640        650
PSVLKPGQSH LEAVAEAANC ISLGDIVEKK IRSSEQLWSL LPLHAVLSSV 
       660        670        680        690        700
YPASKVAGHM AGRINFTAWL GQNSKSAKYY RLLQEIHYHT RLGTSTDKIG 
       710        720        730        740        750
LRLDYLPTFR KRLLDPFLKQ GADAISSVIE VMDDYYLTKE DWDSIMEFFV 
       760        770        780        790        800
GPDVTTAIIK KIPATVKSGF TRKYNSMTHP VAIYRTGSTI GGGGVGTSTS 
       810        820        830        840        850
TPDFEDVVDA DDNPVPADDE ETQDSSTDLK KDKLIKQKAK PTKRKTATSK 
       860 
PGGSKKRKTK A

Length:861

Mass (Da):94,903

Last modified:October 1, 1994 - v1

Checksum:ⁱA7D9208D66DD9A98

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U03102 Unassigned DNA Translation: AAC48916.1 U26027 Genomic DNA Translation: AAC49060.1 X92441 Genomic DNA Translation: CAA63180.1 Z75125 Genomic DNA Translation: CAA99434.1 BK006948 Genomic DNA Translation: DAA10989.1
PIRⁱ	S44763
NCBI Reference Sequences More...RefSeqⁱ	NP_014860.1, NM_001183636.1

Genome annotation databases

EnsemblFungiⁱ	YOR217W; YOR217W; YOR217W
GeneIDⁱ	854392
KEGGⁱ	sce:YOR217W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P38630	Replication factor C subunit 1		YEAS8		861	UniRef100_P38630
Replication factor C subunit 1		Saccharomyces sp. 'boulardii'		861
Replication factor C subunit 1		YEASC		861
Replication factor C subunit 1		YEAS2		861
YOR217Wp-like protein		YEAS6		433

Protein	Similar proteins		Species	Score	Length	Source
P38630	Replication factor C subunit 1		YEAS8		861	UniRef90_P38630
Replication factor C subunit 1		Saccharomyces sp. 'boulardii'		861
Replication factor C subunit 1		YEASC		861
Replication factor C subunit 1		YEAS2		861
YOR217Wp-like protein		YEAS6		433
+3

Protein	Similar proteins		Species	Score	Length	Source
P38630	Replication factor C subunit 1		YEAS8		861	UniRef50_P38630
Replication factor C subunit 1		Saccharomyces sp. 'boulardii'		861
Replication factor C subunit 1		YEASC		861
Replication factor C subunit 1		YEAS2		861
YOR217Wp-like protein		YEAS6		433
+15

Entry informationⁱ

Entry nameⁱ	RFC1_YEAST
Accessionⁱ	P38630Primary (citable) accession number: P38630 Secondary accession number(s): D6W2S3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
	Last sequence update:	October 1, 1994
	Last modified:	September 12, 2018
	This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

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UniParc

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UniRef

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Supporting data

UniProtKB - P38630 (RFC1_YEAST)

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Replication factor C subunit 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ