RFC3

Functionⁱ

Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair. RFC3 supplies a catalytic site to the ATP site of RFC4.4 Publications

Miscellaneous

Present with 3140 molecules/cell in log phase SD medium.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	20	ATP	1
Binding siteⁱ	28	ATP; via amide nitrogen and carbonyl oxygen	1
Binding siteⁱ	148	ATP	1
Binding siteⁱ	206	ATP	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	16 – 19	ATP		4
Nucleotide bindingⁱ	53 – 61	ATP		9

GO - Molecular functionⁱ

ATPase activity
Source: SGD
Inferred from direct assayⁱ
- 8302859
ATP binding Source: UniProtKB-KW
DNA binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

leading strand elongation
Source: SGD
Inferred from direct assayⁱ
- 1346062
mismatch repair
Source: SGD
Traceable author statementⁱ
- 10072354
sister chromatid cohesion
Source: SGD
Inferred from physical interactionⁱ
- 11389843

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Cell cycle, DNA replication
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-33280-MONOMER
Reactomeⁱ	R-SCE-110312 Translesion synthesis by REV1 R-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex R-SCE-110320 Translesion Synthesis by POLH R-SCE-174411 Polymerase switching on the C-strand of the telomere R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair R-SCE-5655862 Translesion synthesis by POLK R-SCE-5656121 Translesion synthesis by POLI R-SCE-5656169 Termination of translesion DNA synthesis R-SCE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER R-SCE-5696400 Dual Incision in GG-NER R-SCE-6782135 Dual incision in TC-NER R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-69091 Polymerase switching

BioCycⁱ

YEAST:G3O-33280-MONOMER

Reactomeⁱ

R-SCE-110312 Translesion synthesis by REV1
R-SCE-110314 Recognition of DNA damage by PCNA-containing replication complex
R-SCE-110320 Translesion Synthesis by POLH
R-SCE-174411 Polymerase switching on the C-strand of the telomere
R-SCE-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-SCE-5655862 Translesion synthesis by POLK
R-SCE-5656121 Translesion synthesis by POLI
R-SCE-5656169 Termination of translesion DNA synthesis
R-SCE-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-SCE-5696400 Dual Incision in GG-NER
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-69091 Polymerase switching

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Replication factor C subunit 3 Short name: Replication factor C3 Alternative name(s): Activator 1 40 kDa subunit
Gene namesⁱ	Name:RFC3 Ordered Locus Names:YNL290W ORF Names:N0533
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome XIV

Organism-specific databases

EuPathDBⁱ	FungiDB:YNL290W
Saccharomyces Genome Database More...SGDⁱ	S000005234 RFC3

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000121756	2 – 340	Replication factor C subunit 3Add BLAST		339

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	2	N-acetylserineCombined sources		1

Keywords - PTMⁱ

Acetylation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P38629
PaxDbⁱ	P38629
PRIDEⁱ	P38629

PTM databases

iPTMnetⁱ	P38629

iPTMnetⁱ

P38629

Interactionⁱ

Subunit structureⁱ

Replication factor C (RFC) is a heteropentamer of subunits RFC1, RFC2, RFC3, RFC4 and RFC5 and forms a complex with POL30/PCNA in the presence of ATP. Component of the RAD24-RFC complex which consists of RAD14, RFC2, RFC3, RFC4 and RFC5 and associates with the checkpoint clamp DDC1:MEC3:RAD17 complex. Component of the ELG1-RFC complex which consists of ELG1, RFC2, RFC3, RFC4 and RFC5. Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. RFC3 interacts with ECO1 and POL30/PCNA.9 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CTF18	P49956	3	EBI-15000,EBI-4560
CTF8	P38877	2	EBI-15000,EBI-5216
RAD24	P32641	5	EBI-15000,EBI-14675
RFC2	P40348	4	EBI-15000,EBI-14992

Protein-protein interaction databases

BioGridⁱ	35547, 98 interactors
ComplexPortalⁱ	CPX-1731 CTF18-RFC complex CPX-1807 Rad17 RFC-like complex CPX-422 ELG1-RFC complex CPX-545 DNA replication factor C complex
Database of interacting proteins More...DIPⁱ	DIP-2529N
IntActⁱ	P38629, 40 interactors
Molecular INTeraction database More...MINTⁱ	P38629
STRINGⁱ	4932.YNL290W

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	15 – 18	Combined sources	4
Helixⁱ	24 – 26	Combined sources	3
Helixⁱ	31 – 42	Combined sources	12
Beta strandⁱ	49 – 52	Combined sources	4
Beta strandⁱ	54 – 58	Combined sources	5
Helixⁱ	59 – 71	Combined sources	13
Helixⁱ	75 – 78	Combined sources	4
Beta strandⁱ	79 – 82	Combined sources	4
Helixⁱ	90 – 94	Combined sources	5
Helixⁱ	96 – 102	Combined sources	7
Beta strandⁱ	106 – 108	Combined sources	3
Beta strandⁱ	112 – 116	Combined sources	5
Helixⁱ	119 – 121	Combined sources	3
Helixⁱ	124 – 136	Combined sources	13
Turnⁱ	137 – 140	Combined sources	4
Beta strandⁱ	141 – 148	Combined sources	8
Helixⁱ	150 – 152	Combined sources	3
Helixⁱ	155 – 158	Combined sources	4
Beta strandⁱ	161 – 165	Combined sources	5
Helixⁱ	171 – 183	Combined sources	13
Turnⁱ	184 – 186	Combined sources	3
Helixⁱ	191 – 201	Combined sources	11
Helixⁱ	205 – 211	Combined sources	7
Turnⁱ	212 – 218	Combined sources	7
Beta strandⁱ	221 – 223	Combined sources	3
Helixⁱ	229 – 235	Combined sources	7
Helixⁱ	241 – 252	Combined sources	12
Helixⁱ	256 – 268	Combined sources	13
Turnⁱ	269 – 271	Combined sources	3
Helixⁱ	274 – 285	Combined sources	12
Helixⁱ	293 – 310	Combined sources	18
Helixⁱ	316 – 330	Combined sources	15

Show more details

3D structure databases

ProteinModelPortalⁱ	P38629
SMRⁱ	P38629
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P38629

EvolutionaryTraceⁱ

P38629

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the activator 1 small subunits family.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000075072
HOGENOMⁱ	HOG000224152
InParanoidⁱ	P38629
KEGG Orthology (KO) More...KOⁱ	K10756
OMAⁱ	CTGNPHP
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C39MN

Family and domain databases

InterProⁱ	View protein in InterPro IPR003593 AAA+_ATPase IPR003959 ATPase_AAA_core IPR008921 DNA_pol3_clamp-load_cplx_C IPR027417 P-loop_NTPase IPR013748 Rep_factorC_C
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00004 AAA, 1 hit PF08542 Rep_fac_C, 1 hit
SMARTⁱ	View protein in SMART SM00382 AAA, 1 hit
SUPFAMⁱ	SSF48019 SSF48019, 1 hit SSF52540 SSF52540, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P38629-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSTSTEKRSK ENLPWVEKYR PETLDEVYGQ NEVITTVRKF VDEGKLPHLL 
        60         70         80         90        100
FYGPPGTGKT STIVALAREI YGKNYSNMVL ELNASDDRGI DVVRNQIKDF 
       110        120        130        140        150
ASTRQIFSKG FKLIILDEAD AMTNAAQNAL RRVIERYTKN TRFCVLANYA 
       160        170        180        190        200
HKLTPALLSR CTRFRFQPLP QEAIERRIAN VLVHEKLKLS PNAEKALIEL 
       210        220        230        240        250
SNGDMRRVLN VLQSCKATLD NPDEDEISDD VIYECCGAPR PSDLKAVLKS 
       260        270        280        290        300
ILEDDWGTAH YTLNKVRSAK GLALIDLIEG IVKILEDYEL QNEETRVHLL 
       310        320        330        340
TKLADIEYSI SKGGNDQIQG SAVIGAIKAS FENETVKANV

Length:340

Mass (Da):38,204

Last modified:October 1, 1994 - v1

Checksum:ⁱ0170EDABAB5CEA52

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L18755 Genomic DNA Translation: AAA34969.1 U26029 Genomic DNA Translation: AAC49062.1 U23084 Genomic DNA Translation: AAC49110.1 Z71566 Genomic DNA Translation: CAA96207.1 BK006947 Genomic DNA Translation: DAA10269.1
PIRⁱ	A36988
NCBI Reference Sequences More...RefSeqⁱ	NP_014109.1, NM_001183128.1

Genome annotation databases

EnsemblFungiⁱ	YNL290W; YNL290W; YNL290W
GeneIDⁱ	855426
KEGGⁱ	sce:YNL290W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P38629	Rfc3p		YEASC		340	UniRef100_P38629
YNL290Wp-like protein (Fragment)		YEAS6		181

Protein	Similar proteins		Species	Score	Length	Source
P38629	Rfc3p		YEASC		340	UniRef90_P38629
YNL290Wp-like protein (Fragment)		YEAS6		181
RFC3-like protein		SACEU		354
Rfc3p		SACCK		340
RFC3-like protein		SACK1		340
+13

Protein	Similar proteins		Species	Score	Length	Source
P38629	Rfc3p		YEASC		340	UniRef50_P38629
YNL290Wp-like protein (Fragment)		YEAS6		181
RFC3-like protein		SACEU		354
Rfc3p		YEASB		340
Replication factor C subunit 3		YEAS1		340
+63

Entry informationⁱ

Entry nameⁱ	RFC3_YEAST
Accessionⁱ	P38629Primary (citable) accession number: P38629 Secondary accession number(s): D6W0Q3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
	Last sequence update:	October 1, 1994
	Last modified:	September 12, 2018
	This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P38629 (RFC3_YEAST)

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Replication factor C subunit 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Cytosol

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ