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Protein

NADH-cytochrome b5 reductase 1

Gene

CBR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Electron donor reductase for cytochrome b5. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes. Plays a role in bud morphology.1 Publication

Miscellaneous

Present with 4820 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.

Cofactori

Enzyme regulationi

Competitively inhibited by NAD+. Inhibited by mercurials such as p-chloromercuribenzoate (PCMB) and HgCl2. Enzymatic activity increases under anaerobic conditions.2 Publications

Kineticsi

  1. KM=6.3 µM for NADH1 Publication
  2. KM=0.8 µM for cytochrome b51 Publication

    pH dependencei

    Optimum pH is 5.6. Active from pH 5 to 7.5.1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi122 – 137FADBy similarityAdd BLAST16
    Nucleotide bindingi148 – 180FADBy similarityAdd BLAST33

    GO - Molecular functioni

    • cytochrome-b5 reductase activity, acting on NAD(P)H Source: SGD

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein, NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:YIL043C-MONOMER
    YEAST:YIL043C-MONOMER
    ReactomeiR-SCE-114608 Platelet degranulation
    R-SCE-196836 Vitamin C (ascorbate) metabolism
    R-SCE-6798695 Neutrophil degranulation
    SABIO-RKiP38626

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-cytochrome b5 reductase 1 (EC:1.6.2.2)
    Alternative name(s):
    Microsomal cytochrome b reductase
    P35
    Gene namesi
    Name:CBR1
    Synonyms:CBR, CBR5
    Ordered Locus Names:YIL043C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome IX

    Organism-specific databases

    EuPathDBiFungiDB:YIL043C
    SGDiS000001305 CBR1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei7 – 27HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001676271 – 284NADH-cytochrome b5 reductase 1Add BLAST284

    Proteomic databases

    MaxQBiP38626
    PaxDbiP38626
    PRIDEiP38626

    Expressioni

    Inductioni

    Protein levels are highest during exponential growth phase and lowest in stationary phase.1 Publication

    Interactioni

    Subunit structurei

    Monomer. Interacts with STE20.2 Publications

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi34947, 117 interactors
    IntActiP38626, 37 interactors
    MINTiP38626
    STRINGi4932.YIL043C

    Structurei

    3D structure databases

    ProteinModelPortaliP38626
    SMRiP38626
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini38 – 142FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST105

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00390000008881
    HOGENOMiHOG000175005
    InParanoidiP38626
    KOiK00326
    OMAiHNTAIYR
    OrthoDBiEOG092C43G8

    Family and domain databases

    Gene3Di3.40.50.80, 1 hit
    InterProiView protein in InterPro
    IPR017927 Fd_Rdtase_FAD-bd
    IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
    IPR039261 FNR_nucleotide_bd
    IPR001834 NADH-Cyt_B5_reductase
    IPR008333 OxRdtase_FAD-bd_dom
    IPR001433 OxRdtase_FAD/NAD-bd
    IPR017938 Riboflavin_synthase-like_b-brl
    PfamiView protein in Pfam
    PF00970 FAD_binding_6, 1 hit
    PF00175 NAD_binding_1, 1 hit
    PRINTSiPR00406 CYTB5RDTASE
    PR00371 FPNCR
    SUPFAMiSSF52343 SSF52343, 1 hit
    SSF63380 SSF63380, 1 hit
    PROSITEiView protein in PROSITE
    PS51384 FAD_FR, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P38626-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAIDAQKLVV VIVIVVVPLL FKFIIGPKTK PVLDPKRNDF QSFPLVEKTI
    60 70 80 90 100
    LTHNTSMYKF GLPHADDVLG LPIGQHIVIK ANINGKDITR SYTPTSLDGD
    110 120 130 140 150
    TKGNFELLVK SYPTGNVSKM IGELKIGDSI QIKGPRGNYH YERNCRSHLG
    160 170 180 190 200
    MIAGGTGIAP MYQIMKAIAM DPHDTTKVSL VFGNVHEEDI LLKKELEALV
    210 220 230 240 250
    AMKPSQFKIV YYLDSPDRED WTGGVGYITK DVIKEHLPAA TMDNVQILIC
    260 270 280
    GPPAMVASVR RSTVDLGFRR SKPLSKMEDQ VFVF
    Length:284
    Mass (Da):31,494
    Last modified:April 29, 2008 - v2
    Checksum:i0E71E7AB083BB623
    GO

    Sequence cautioni

    The sequence CAA82214 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA86908 differs from that shown. Reason: Erroneous initiation.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z28365 Genomic DNA Translation: CAA82214.1 Different initiation.
    Z46861 Genomic DNA Translation: CAA86908.1 Different initiation.
    BK006942 Genomic DNA Translation: DAA08505.1
    PIRiS49935
    RefSeqiNP_012221.2, NM_001179393.1

    Genome annotation databases

    EnsemblFungiiYIL043C; YIL043C; YIL043C
    GeneIDi854768
    KEGGisce:YIL043C

    Similar proteinsi

    Entry informationi

    Entry nameiNCB5R_YEAST
    AccessioniPrimary (citable) accession number: P38626
    Secondary accession number(s): D6VVN9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: April 29, 2008
    Last modified: July 18, 2018
    This is version 157 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

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