UniProtKB - P38623 (RCK2_YEAST)
Protein
Serine/threonine-protein kinase RCK2
Gene
RCK2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Serine/threonine-protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment.1 Publication
Miscellaneous
Present with 1790 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
Activity regulationi
Activated by Ser-520 phosphorylation by HOG1.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 201 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 313 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 169 – 177 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: UniProtKB-KW
- calmodulin-dependent protein kinase activity Source: GO_Central
- protein kinase activity Source: SGD
- protein serine/threonine kinase activity Source: SGD
GO - Biological processi
- cellular response to oxidative stress Source: SGD
- osmosensory signaling pathway Source: SGD
- protein phosphorylation Source: SGD
- regulation of meiotic nuclear division Source: SGD
Keywordsi
Molecular function | Calmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 984 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase RCK2 (EC:2.7.11.1)Alternative name(s): CAM kinase-like protein kinase CLK1 |
Gene namesi | Name:RCK2 Synonyms:CLK1, CMK3 Ordered Locus Names:YLR248W ORF Names:L9672.6 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YLR248W |
SGDi | S000004238, RCK2 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086604 | 1 – 610 | Serine/threonine-protein kinase RCK2Add BLAST | 610 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 46 | PhosphoserineCombined sources | 1 | |
Modified residuei | 50 | PhosphoserineCombined sources | 1 | |
Modified residuei | 187 | PhosphoserineCombined sources | 1 | |
Modified residuei | 350 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 520 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Autophosphorylated. Phosphorylated by HOG1 at Ser-520 after osmotic stress.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P38623 |
PaxDbi | P38623 |
PRIDEi | P38623 |
PTM databases
iPTMneti | P38623 |
Interactioni
Binary interactionsi
P38623
With | #Exp. | IntAct |
---|---|---|
HOG1 [P32485] | 4 | EBI-14885,EBI-8437 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 31516, 133 interactors |
DIPi | DIP-5079N |
IntActi | P38623, 11 interactors |
MINTi | P38623 |
STRINGi | 4932.YLR248W |
Miscellaneous databases
RNActi | P38623, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 163 – 478 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 316 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 493 – 506 | Calmodulin-bindingSequence analysisAdd BLAST | 14 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota |
GeneTreei | ENSGT00940000176587 |
HOGENOMi | CLU_006421_3_0_1 |
InParanoidi | P38623 |
OMAi | FKQGYRG |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P38623-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKIKALFSK KKPDQADLSQ ESKKPFKGKT RSSGTNNKDV SQITSSPKKS
60 70 80 90 100
FQDKNIVQYP SVVADDHHMK SLTDELVTTI DSDSSPSDNI TTENVETVTS
110 120 130 140 150
VPAIDVHESS EGQLSSDPLI SDESLSEQSE IISDIQDDST DDDNMEDEIP
160 170 180 190 200
EKSFLEQKEL IGYKLINKIG EGAFSKVFRA IPAKNSSNEF LTKNYKAVAI
210 220 230 240 250
KVIKKADLSS INGDHRKKDK GKDSTKTSSR DQVLKEVALH KTVSAGCSQI
260 270 280 290 300
VAFIDFQETD SYYYIIQELL TGGEIFGEIV RLTYFSEDLS RHVIKQLALA
310 320 330 340 350
VKHMHSLGVV HRDIKPENLL FEPIEFTRSI KPKLRKSDDP QTKADEGIFT
360 370 380 390 400
PGVGGGGIGI VKLADFGLSK QIFSKNTKTP CGTVGYTAPE VVKDEHYSMK
410 420 430 440 450
VDMWGIGCVL YTMLCGFPPF YDEKIDTLTE KISRGEYTFL KPWWDEISAG
460 470 480 490 500
AKNAVAKLLE LEPSKRYDID QFLDDPWLNT FDCLPKEGES SQKKAGTSER
510 520 530 540 550
RHPHKKQFQL FQRDSSLLFS PAAVAMRDAF DIGNAVKRTE EDRMGTRGGL
560 570 580 590 600
GSLAEDEELE DSYSGAQGDE QLEQNMFQLT LDTSTILQRR KKVQENDVGP
610
TIPISATIRE
Sequence cautioni
The sequence CAA50389 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 109 | S → N in CAA50389 (PubMed:8112585).Curated | 1 | |
Sequence conflicti | 109 | S → N in AAA64421 (PubMed:8939941).Curated | 1 | |
Sequence conflicti | 188 | N → H in CAA50389 (PubMed:8112585).Curated | 1 | |
Sequence conflicti | 188 | N → H in AAA64421 (PubMed:8939941).Curated | 1 | |
Sequence conflicti | 233 | V → A in CAA50389 (PubMed:8112585).Curated | 1 | |
Sequence conflicti | 233 | V → A in AAA64421 (PubMed:8939941).Curated | 1 | |
Sequence conflicti | 328 | R → P in CAA50389 (PubMed:8112585).Curated | 1 | |
Sequence conflicti | 328 | R → P in AAA64421 (PubMed:8939941).Curated | 1 | |
Sequence conflicti | 456 | A → S in CAA50389 (PubMed:8112585).Curated | 1 | |
Sequence conflicti | 456 | A → S in AAA64421 (PubMed:8939941).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X71065 Genomic DNA Translation: CAA50389.1 Frameshift. U23464 Genomic DNA Translation: AAA64421.1 U20865 Genomic DNA Translation: AAB67392.1 BK006945 Genomic DNA Translation: DAA09562.1 |
PIRi | S59394 |
RefSeqi | NP_013349.1, NM_001182135.1 |
Genome annotation databases
EnsemblFungii | YLR248W_mRNA; YLR248W; YLR248W |
GeneIDi | 850950 |
KEGGi | sce:YLR248W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X71065 Genomic DNA Translation: CAA50389.1 Frameshift. U23464 Genomic DNA Translation: AAA64421.1 U20865 Genomic DNA Translation: AAB67392.1 BK006945 Genomic DNA Translation: DAA09562.1 |
PIRi | S59394 |
RefSeqi | NP_013349.1, NM_001182135.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 31516, 133 interactors |
DIPi | DIP-5079N |
IntActi | P38623, 11 interactors |
MINTi | P38623 |
STRINGi | 4932.YLR248W |
PTM databases
iPTMneti | P38623 |
Proteomic databases
MaxQBi | P38623 |
PaxDbi | P38623 |
PRIDEi | P38623 |
Genome annotation databases
EnsemblFungii | YLR248W_mRNA; YLR248W; YLR248W |
GeneIDi | 850950 |
KEGGi | sce:YLR248W |
Organism-specific databases
EuPathDBi | FungiDB:YLR248W |
SGDi | S000004238, RCK2 |
Phylogenomic databases
eggNOGi | KOG0032, Eukaryota |
GeneTreei | ENSGT00940000176587 |
HOGENOMi | CLU_006421_3_0_1 |
InParanoidi | P38623 |
OMAi | FKQGYRG |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 984 |
Miscellaneous databases
PROi | PR:P38623 |
RNActi | P38623, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RCK2_YEAST | |
Accessioni | P38623Primary (citable) accession number: P38623 Secondary accession number(s): D6VYP6, Q02532, Q06557 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 5, 2010 | |
Last modified: | December 2, 2020 | |
This is version 193 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD