UniProtKB - P38604 (ERG7_YEAST)
Protein
Lanosterol synthase
Gene
ERG7
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus.
Miscellaneous
Present with 2193 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:5.4.99.7
Activity regulationi
ERG27 is required for activity.1 Publication
pH dependencei
Optimum pH is 7.1 Publication
Temperature dependencei
Optimum temperature is 35 degrees Celsius.1 Publication
: lanosterol biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Squalene synthase (ERG9)
- Squalene monooxygenase (ERG1)
- Lanosterol synthase (ERG7)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 456 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- beta-amyrin synthase activity Source: InterPro
- lanosterol synthase activity Source: SGD
GO - Biological processi
- ergosterol biosynthetic process Source: SGD
- triterpenoid biosynthetic process Source: InterPro
Keywordsi
Molecular function | Isomerase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
Enzyme and pathway databases
BioCyci | MetaCyc:YHR072W-MONOMER |
BRENDAi | 5.4.99.7, 984 |
Reactomei | R-SCE-191273, Cholesterol biosynthesis |
SABIO-RKi | P38604 |
UniPathwayi | UPA00767;UER00753 |
Names & Taxonomyi
Protein namesi | Recommended name: Lanosterol synthase (EC:5.4.99.7)Alternative name(s): 2,3-epoxysqualene--lanosterol cyclase Oxidosqualene--lanosterol cyclase Short name: OSC |
Gene namesi | Name:ERG7 Ordered Locus Names:YHR072W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YHR072W |
SGDi | S000001114, ERG7 |
Subcellular locationi
Endoplasmic reticulum
Other locations
Note: Predominantly in lipid particles.
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- lipid droplet Source: SGD
Keywords - Cellular componenti
Endoplasmic reticulum, Lipid droplet, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 457 | C → D: Reduces thermal stability and enzyme activity; when associated with A-526 or C-526. No effect; when associated with D-526 or Q-526. 1 Publication | 1 | |
Mutagenesisi | 526 | E → A or C: Reduces thermal stability and enzyme activity; when associated with D-457. 1 Publication | 1 | |
Mutagenesisi | 526 | E → D or Q: No effect; when associated with D-457. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL5355 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000072657 | 2 – 731 | Lanosterol synthaseAdd BLAST | 730 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
MaxQBi | P38604 |
PaxDbi | P38604 |
PRIDEi | P38604 |
PTM databases
iPTMneti | P38604 |
Interactioni
Binary interactionsi
P38604
With | #Exp. | IntAct |
---|---|---|
ERG27 [Q12452] | 4 | EBI-6572,EBI-38132 |
Protein-protein interaction databases
BioGRIDi | 36505, 287 interactors |
DIPi | DIP-8319N |
IntActi | P38604, 19 interactors |
MINTi | P38604 |
STRINGi | 4932.YHR072W |
Chemistry databases
BindingDBi | P38604 |
Miscellaneous databases
RNActi | P38604, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 125 – 167 | PFTB 1Add BLAST | 43 | |
Repeati | 566 – 603 | PFTB 2Add BLAST | 38 | |
Repeati | 615 – 661 | PFTB 3Add BLAST | 47 |
Sequence similaritiesi
Belongs to the terpene cyclase/mutase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0497, Eukaryota |
GeneTreei | ENSGT00390000011570 |
HOGENOMi | CLU_009074_2_1_1 |
InParanoidi | P38604 |
OMAi | CWARQTI |
Family and domain databases
CDDi | cd02892, SQCY_1, 1 hit |
InterProi | View protein in InterPro IPR032696, SQ_cyclase_C IPR032697, SQ_cyclase_N IPR018333, Squalene_cyclase IPR002365, Terpene_synthase_CS IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF13243, SQHop_cyclase_C, 1 hit PF13249, SQHop_cyclase_N, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits |
TIGRFAMsi | TIGR01787, squalene_cyclas, 1 hit |
PROSITEi | View protein in PROSITE PS01074, TERPENE_SYNTHASES, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P38604-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEFYSDTIG LPKTDPRLWR LRTDELGRES WEYLTPQQAA NDPPSTFTQW
60 70 80 90 100
LLQDPKFPQP HPERNKHSPD FSAFDACHNG ASFFKLLQEP DSGIFPCQYK
110 120 130 140 150
GPMFMTIGYV AVNYIAGIEI PEHERIELIR YIVNTAHPVD GGWGLHSVDK
160 170 180 190 200
STVFGTVLNY VILRLLGLPK DHPVCAKARS TLLRLGGAIG SPHWGKIWLS
210 220 230 240 250
ALNLYKWEGV NPAPPETWLL PYSLPMHPGR WWVHTRGVYI PVSYLSLVKF
260 270 280 290 300
SCPMTPLLEE LRNEIYTKPF DKINFSKNRN TVCGVDLYYP HSTTLNIANS
310 320 330 340 350
LVVFYEKYLR NRFIYSLSKK KVYDLIKTEL QNTDSLCIAP VNQAFCALVT
360 370 380 390 400
LIEEGVDSEA FQRLQYRFKD ALFHGPQGMT IMGTNGVQTW DCAFAIQYFF
410 420 430 440 450
VAGLAERPEF YNTIVSAYKF LCHAQFDTEC VPGSYRDKRK GAWGFSTKTQ
460 470 480 490 500
GYTVADCTAE AIKAIIMVKN SPVFSEVHHM ISSERLFEGI DVLLNLQNIG
510 520 530 540 550
SFEYGSFATY EKIKAPLAME TLNPAEVFGN IMVEYPYVEC TDSSVLGLTY
560 570 580 590 600
FHKYFDYRKE EIRTRIRIAI EFIKKSQLPD GSWYGSWGIC FTYAGMFALE
610 620 630 640 650
ALHTVGETYE NSSTVRKGCD FLVSKQMKDG GWGESMKSSE LHSYVDSEKS
660 670 680 690 700
LVVQTAWALI ALLFAEYPNK EVIDRGIDLL KNRQEESGEW KFESVEGVFN
710 720 730
HSCAIEYPSY RFLFPIKALG MYSRAYETHT L
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 61 | H → N in AAA16975 (PubMed:8134375).Curated | 1 | |
Sequence conflicti | 530 | N → D in AAA16975 (PubMed:8134375).Curated | 1 | |
Sequence conflicti | 530 | N → D in AAB68891 (PubMed:8091229).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 359 – 731 | Missing in strain: SGL9; loss of activity. 1 PublicationAdd BLAST | 373 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U04841 Unassigned DNA Translation: AAA16975.1 U23488 Genomic DNA Translation: AAA64377.1 U60996 Genomic DNA Translation: AAB08472.1 U10556 Genomic DNA Translation: AAB68891.1 AY693043 Genomic DNA Translation: AAT93062.1 BK006934 Genomic DNA Translation: DAA06765.2 |
PIRi | S46813 |
RefSeqi | NP_011939.2, NM_001179202.2 |
Genome annotation databases
EnsemblFungii | YHR072W_mRNA; YHR072W; YHR072W |
GeneIDi | 856470 |
KEGGi | sce:YHR072W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U04841 Unassigned DNA Translation: AAA16975.1 U23488 Genomic DNA Translation: AAA64377.1 U60996 Genomic DNA Translation: AAB08472.1 U10556 Genomic DNA Translation: AAB68891.1 AY693043 Genomic DNA Translation: AAT93062.1 BK006934 Genomic DNA Translation: DAA06765.2 |
PIRi | S46813 |
RefSeqi | NP_011939.2, NM_001179202.2 |
3D structure databases
SMRi | P38604 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36505, 287 interactors |
DIPi | DIP-8319N |
IntActi | P38604, 19 interactors |
MINTi | P38604 |
STRINGi | 4932.YHR072W |
Chemistry databases
BindingDBi | P38604 |
ChEMBLi | CHEMBL5355 |
PTM databases
iPTMneti | P38604 |
Proteomic databases
MaxQBi | P38604 |
PaxDbi | P38604 |
PRIDEi | P38604 |
Genome annotation databases
EnsemblFungii | YHR072W_mRNA; YHR072W; YHR072W |
GeneIDi | 856470 |
KEGGi | sce:YHR072W |
Organism-specific databases
EuPathDBi | FungiDB:YHR072W |
SGDi | S000001114, ERG7 |
Phylogenomic databases
eggNOGi | KOG0497, Eukaryota |
GeneTreei | ENSGT00390000011570 |
HOGENOMi | CLU_009074_2_1_1 |
InParanoidi | P38604 |
OMAi | CWARQTI |
Enzyme and pathway databases
UniPathwayi | UPA00767;UER00753 |
BioCyci | MetaCyc:YHR072W-MONOMER |
BRENDAi | 5.4.99.7, 984 |
Reactomei | R-SCE-191273, Cholesterol biosynthesis |
SABIO-RKi | P38604 |
Miscellaneous databases
PROi | PR:P38604 |
RNActi | P38604, protein |
Family and domain databases
CDDi | cd02892, SQCY_1, 1 hit |
InterProi | View protein in InterPro IPR032696, SQ_cyclase_C IPR032697, SQ_cyclase_N IPR018333, Squalene_cyclase IPR002365, Terpene_synthase_CS IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF13243, SQHop_cyclase_C, 1 hit PF13249, SQHop_cyclase_N, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits |
TIGRFAMsi | TIGR01787, squalene_cyclas, 1 hit |
PROSITEi | View protein in PROSITE PS01074, TERPENE_SYNTHASES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ERG7_YEAST | |
Accessioni | P38604Primary (citable) accession number: P38604 Secondary accession number(s): D3DL21, Q92327 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 180 of the entry and version 6 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VIII
Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names