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Protein

Heat shock factor protein 2

Gene

Hsf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi7 – 112By similarityAdd BLAST106

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processStress response, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein 2
Short name:
HSF 2
Alternative name(s):
Heat shock transcription factor 2
Short name:
HSTF 2
Gene namesi
Name:Hsf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:96239 Hsf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001245701 – 535Heat shock factor protein 2Add BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki2Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki82Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki135Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki139Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki151Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki210Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki218Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki237Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP38533
PaxDbiP38533
PRIDEiP38533

PTM databases

iPTMnetiP38533
PhosphoSitePlusiP38533

Expressioni

Tissue specificityi

Isoform alpha is expressed predominantly in testis while isoform beta is expressed predominantly in heart and brain.1 Publication

Developmental stagei

In the 7-day-old testis, isoform beta is expressed at significantly higher levels than isoform alpha. As development proceeds, the levels of isoform alpha gradually increase so that it is the predominant isoform in mature testes.1 Publication

Gene expression databases

BgeeiENSMUSG00000019878 Expressed in 55 organ(s), highest expression level in head
CleanExiMM_HSF2
ExpressionAtlasiP38533 baseline and differential
GenevisibleiP38533 MM

Interactioni

Subunit structurei

DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078761

Structurei

3D structure databases

ProteinModelPortaliP38533
SMRiP38533
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 192Hydrophobic repeat HR-A/BAdd BLAST74
Regioni359 – 384Hydrophobic repeat HR-CAdd BLAST26

Sequence similaritiesi

Belongs to the HSF family.Curated

Phylogenomic databases

eggNOGiKOG0627 Eukaryota
COG5169 LUCA
GeneTreeiENSGT00390000001182
HOGENOMiHOG000253917
HOVERGENiHBG005999
InParanoidiP38533
KOiK09415
OrthoDBiEOG091G087O
PhylomeDBiP38533
TreeFamiTF330401

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR027712 HSF2
IPR000232 HSF_DNA-bd
IPR027725 HSF_fam
IPR010542 Vert_HSTF_C
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10015 PTHR10015, 1 hit
PTHR10015:SF185 PTHR10015:SF185, 1 hit
PfamiView protein in Pfam
PF00447 HSF_DNA-bind, 1 hit
PF06546 Vert_HS_TF, 1 hit
PRINTSiPR00056 HSFDOMAIN
SMARTiView protein in SMART
SM00415 HSF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00434 HSF_DOMAIN, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform Alpha (identifier: P38533-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP
60 70 80 90 100
KYFKHNNMAS FVRQLNMYGF RKVVHIESGI IKQERDGPVE FQHPYFKQGQ
110 120 130 140 150
DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ ETIESRLSEL
160 170 180 190 200
KSENESLWKE VSELRAKHAQ QQQVIRKIVQ FIVTLVQNNQ LVSLKRKRPL
210 220 230 240 250
LLNTNGAPKK NLYQHIVKEP TDNHHHKVPH SRTEGLKSRE RISDDIIIYD
260 270 280 290 300
VTDDNVDEEN IPVIPETNED VVVDSSNQYP DIVIVEDDNE DEYAPVIQSG
310 320 330 340 350
EQSEPAREPL RVGSAGSSSP LMSSAVQLNG SSSLTSEDPV TMMDSILNDN
360 370 380 390 400
INLLGKVELL DYLDSIDCSL EDFQAMLSGR QFSIDPDLLV DLFTSSVQMN
410 420 430 440 450
PTDNIKYTKS ENKGLEATKS SVVQHVSEEG RKSKSKPDKQ LIQYTAFPLL
460 470 480 490 500
AFLDGNSASA IEQGSTTASS EVVPSVDKPI EVDELLDSSL DPEPTQSKLV
510 520 530
RLEPLTEAEA SEATLFYLCE LAPAPLDSDM PLLDS
Length:535
Mass (Da):60,225
Last modified:August 30, 2002 - v2
Checksum:i47972520402ABB13
GO
Isoform Beta (identifier: P38533-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     392-409: Missing.

Show »
Length:517
Mass (Da):58,155
Checksum:i098965BBCDF4E1B0
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2P8F8A0A1W2P8F8_MOUSE
Heat shock factor protein 2
Hsf2
478Annotation score:
A0A1W2P851A0A1W2P851_MOUSE
Heat shock factor protein 2
Hsf2
246Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002417392 – 409Missing in isoform Beta. 3 PublicationsAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61754 mRNA Translation: CAA43893.1
AF045627
, AF045615, AF045616, AF045617, AF045618, AF045619, AF045620, AF045621, AF045622, AF045623, AF045624, AF045625, AF045626 Genomic DNA Translation: AAD02417.1
S79629 mRNA Translation: AAB35307.1
BC018414 mRNA Translation: AAH18414.1
CCDSiCCDS23852.1 [P38533-2]
PIRiB40583
RefSeqiNP_032323.3, NM_008297.3 [P38533-2]
UniGeneiMm.261395
Mm.447362

Genome annotation databases

EnsembliENSMUST00000079833; ENSMUSP00000078761; ENSMUSG00000019878 [P38533-2]
GeneIDi15500
KEGGimmu:15500
UCSCiuc007fck.2 mouse [P38533-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61754 mRNA Translation: CAA43893.1
AF045627
, AF045615, AF045616, AF045617, AF045618, AF045619, AF045620, AF045621, AF045622, AF045623, AF045624, AF045625, AF045626 Genomic DNA Translation: AAD02417.1
S79629 mRNA Translation: AAB35307.1
BC018414 mRNA Translation: AAH18414.1
CCDSiCCDS23852.1 [P38533-2]
PIRiB40583
RefSeqiNP_032323.3, NM_008297.3 [P38533-2]
UniGeneiMm.261395
Mm.447362

3D structure databases

ProteinModelPortaliP38533
SMRiP38533
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078761

PTM databases

iPTMnetiP38533
PhosphoSitePlusiP38533

Proteomic databases

MaxQBiP38533
PaxDbiP38533
PRIDEiP38533

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079833; ENSMUSP00000078761; ENSMUSG00000019878 [P38533-2]
GeneIDi15500
KEGGimmu:15500
UCSCiuc007fck.2 mouse [P38533-2]

Organism-specific databases

CTDi3298
MGIiMGI:96239 Hsf2

Phylogenomic databases

eggNOGiKOG0627 Eukaryota
COG5169 LUCA
GeneTreeiENSGT00390000001182
HOGENOMiHOG000253917
HOVERGENiHBG005999
InParanoidiP38533
KOiK09415
OrthoDBiEOG091G087O
PhylomeDBiP38533
TreeFamiTF330401

Miscellaneous databases

PROiPR:P38533
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019878 Expressed in 55 organ(s), highest expression level in head
CleanExiMM_HSF2
ExpressionAtlasiP38533 baseline and differential
GenevisibleiP38533 MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR027712 HSF2
IPR000232 HSF_DNA-bd
IPR027725 HSF_fam
IPR010542 Vert_HSTF_C
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10015 PTHR10015, 1 hit
PTHR10015:SF185 PTHR10015:SF185, 1 hit
PfamiView protein in Pfam
PF00447 HSF_DNA-bind, 1 hit
PF06546 Vert_HS_TF, 1 hit
PRINTSiPR00056 HSFDOMAIN
SMARTiView protein in SMART
SM00415 HSF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00434 HSF_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHSF2_MOUSE
AccessioniPrimary (citable) accession number: P38533
Secondary accession number(s): Q64157, Q8VEJ0, Q9Z2L8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: August 30, 2002
Last modified: November 7, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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