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Protein

Immunoglobulin G-binding protein A

Gene

spa

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Avidly binds the Fc region of immunoglobulins (Ig) from most mammalian species, in a non-specific aggregation reaction that can lead to host tissue damage. Also binds the Fab region of Igs, in a region remote from the Ig light chain and the heavy chain constant region, i.e. not a typical Ig-antigen recognition.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIgG-binding protein

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin G-binding protein A
Short name:
IgG-binding protein A
Alternative name(s):
Staphylococcal protein A
Short name:
SpA
Gene namesi
Name:spa
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Biotechnological usei

Important immunodiagnostic reagent because of its ability to bind the Fab and Fc fragments of a wide range of mammalian immunoglobulins.Curated

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
ChainiPRO_000000565537 – 477Immunoglobulin G-binding protein AAdd BLAST441
PropeptideiPRO_0000005656478 – 508Removed by sortasePROSITE-ProRule annotationBy similarityAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei477Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation1

Keywords - PTMi

Peptidoglycan-anchor

Proteomic databases

PRIDEiP38507

Interactioni

Protein-protein interaction databases

IntActiP38507, 1 interactor
MINTiP38507

Structurei

Secondary structure

1508
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP38507
SMRiP38507
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38507

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 92Immunoglobulin-binding region EBy similarityAdd BLAST56
Repeati93 – 153Immunoglobulin-binding region DBy similarityAdd BLAST61
Repeati154 – 211Immunoglobulin-binding region ABy similarityAdd BLAST58
Repeati212 – 269Immunoglobulin-binding region BBy similarityAdd BLAST58
Repeati270 – 327Immunoglobulin-binding region CBy similarityAdd BLAST58
Repeati333 – 3402-18
Repeati341 – 3482-28
Repeati349 – 3562-38
Repeati357 – 3642-48
Repeati365 – 3722-58
Repeati373 – 3802-68
Repeati381 – 3882-78
Repeati389 – 3962-88
Repeati397 – 4052-99
Repeati406 – 4132-108
Domaini413 – 457LysMPROSITE-ProRule annotationAdd BLAST45
Repeati414 – 4212-118
Repeati422 – 4292-128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni333 – 40812 X 8 AA approximate tandem repeatsAdd BLAST76

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi7 – 18YSIRK-G/S signaling motifBy similarityAdd BLAST12
Motifi474 – 478LPXTG sorting signalPROSITE-ProRule annotation5

Domaini

Each of the immunoglobulin-binding region repeats can bind the Fc region of an immunoglobulin.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4106I9N Bacteria
ENOG410XXBQ LUCA

Family and domain databases

CDDicd00118 LysM, 1 hit
Gene3Di3.10.350.10, 1 hit
InterProiView protein in InterPro
IPR019948 Gram-positive_anchor
IPR009063 Ig/albumin-bd_sf
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
IPR005038 Octapeptide
IPR003132 Protein_A_Ig-bd
IPR005877 YSIRK_signal_dom
PfamiView protein in Pfam
PF02216 B, 5 hits
PF00746 Gram_pos_anchor, 1 hit
PF01476 LysM, 1 hit
PF03373 Octapeptide, 11 hits
PF04650 YSIRK_signal, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 1 hit
SUPFAMiSSF46997 SSF46997, 5 hits
TIGRFAMsiTIGR01168 YSIRK_signal, 1 hit
PROSITEiView protein in PROSITE
PS50847 GRAM_POS_ANCHORING, 1 hit
PS51782 LYSM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38507-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV
60 70 80 90 100
LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNKF
110 120 130 140 150
NKDQQSAFYE ILNMPNLNEE QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ
160 170 180 190 200
APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLAE
210 220 230 240 250
AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS
260 270 280 290 300
QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI
310 320 330 340 350
QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDGN KPGKEDGNKP
360 370 380 390 400
GKEDNKKPGK EDGNKPGKED NKKPGKEDGN KPGKEDGNKP GKEDGNKPGK
410 420 430 440 450
EDGNKPGKED GNGVHVVKPG DTVNDIAKAN GTTADKIAAD NKLADKNMIK
460 470 480 490 500
PGQELVVDKK QPANHADANK AQALPETGEE NPFIGTTVFG GLSLALGAAL

LAGRRREL
Length:508
Mass (Da):55,439
Last modified:October 1, 1994 - v1
Checksum:iE78C538D4B5E88F5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti273K → D AA sequence (PubMed:913410).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18264 Genomic DNA Translation: AAA26677.1
PIRiA29605
RefSeqiWP_047211818.1, NZ_UHAY01000002.1

Genome annotation databases

PATRICifig|1280.4807.peg.2341

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18264 Genomic DNA Translation: AAA26677.1
PIRiA29605
RefSeqiWP_047211818.1, NZ_UHAY01000002.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BDCNMR-A212-270[»]
1BDDNMR-A212-270[»]
1EDINMR-A37-92[»]
1EDJNMR-A37-92[»]
1EDKNMR-A37-92[»]
1EDLNMR-A37-92[»]
1FC2X-ray2.80C212-269[»]
1H0TNMR-A213-269[»]
1LP1X-ray2.30B212-269[»]
1Q2NNMR-A212-269[»]
1SS1NMR-A212-270[»]
2JWDNMR-A213-269[»]
2M5ANMR-A213-269[»]
2SPZNMR-A212-269[»]
3MZWX-ray2.90B212-269[»]
4NPDX-ray0.90A270-327[»]
4NPEX-ray1.42A270-327[»]
4NPFX-ray1.49X/Y154-269[»]
4WWIX-ray2.31A/B/C270-327[»]
4Y4YX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d158-211[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d219-459[»]
4Y5ZX-ray2.950/1/2/3/4/5/6/7/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V158-211[»]
0/1/2/3/4/5/6/7/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V219-459[»]
4ZMDX-ray1.87A/B270-327[»]
4ZNCX-ray2.28A/B/C270-327[»]
5CBNX-ray2.30A217-269[»]
5CBOX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L101-153[»]
5COCX-ray2.67A213-267[»]
5EWXX-ray2.60A/B212-266[»]
5H76X-ray2.60A/B/C220-267[»]
5H79X-ray2.70C/D40-210[»]
5H7AX-ray2.70A/B/C/D/E/F/G/H/I/J/K/L157-209[»]
5H7BX-ray3.10A/B157-209[»]
5H7CX-ray2.70A/C99-324[»]
5H7DX-ray2.57A/B/C/D/I/J/M/N220-267[»]
5X3FX-ray3.38A220-269[»]
5XBYX-ray3.25A/B/C/D217-269[»]
ProteinModelPortaliP38507
SMRiP38507
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP38507, 1 interactor
MINTiP38507

Proteomic databases

PRIDEiP38507

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

PATRICifig|1280.4807.peg.2341

Phylogenomic databases

eggNOGiENOG4106I9N Bacteria
ENOG410XXBQ LUCA

Miscellaneous databases

EvolutionaryTraceiP38507
PROiPR:P38507

Family and domain databases

CDDicd00118 LysM, 1 hit
Gene3Di3.10.350.10, 1 hit
InterProiView protein in InterPro
IPR019948 Gram-positive_anchor
IPR009063 Ig/albumin-bd_sf
IPR018392 LysM_dom
IPR036779 LysM_dom_sf
IPR005038 Octapeptide
IPR003132 Protein_A_Ig-bd
IPR005877 YSIRK_signal_dom
PfamiView protein in Pfam
PF02216 B, 5 hits
PF00746 Gram_pos_anchor, 1 hit
PF01476 LysM, 1 hit
PF03373 Octapeptide, 11 hits
PF04650 YSIRK_signal, 1 hit
SMARTiView protein in SMART
SM00257 LysM, 1 hit
SUPFAMiSSF46997 SSF46997, 5 hits
TIGRFAMsiTIGR01168 YSIRK_signal, 1 hit
PROSITEiView protein in PROSITE
PS50847 GRAM_POS_ANCHORING, 1 hit
PS51782 LYSM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPA_STAAU
AccessioniPrimary (citable) accession number: P38507
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 7, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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