UniProtKB - P38500 (NIR_BETPN)
Protein
Ferredoxin--nitrite reductase, chloroplastic
Gene
NIR1
Organism
Betula pendula (European white birch) (Betula verrucosa)
Status
Functioni
Catalytic activityi
- EC:1.7.7.1
Cofactori
Protein has several cofactor binding sites:- sirohemeNote: Binds 1 siroheme per subunit.
- [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity
: nitrate reduction (assimilation) Pathwayi
This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 461 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 467 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 502 | Iron-sulfur (4Fe-4S)By similarity | 1 | |
Metal bindingi | 506 | Iron (siroheme axial ligand)By similarity | 1 | |
Metal bindingi | 506 | Iron-sulfur (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- ferredoxin-nitrite reductase activity Source: UniProtKB-EC
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- nitrate assimilation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Nitrate assimilation, Transport |
Ligand | 4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00653 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:NIR1 |
Organismi | Betula pendula (European white birch) (Betula verrucosa) |
Taxonomic identifieri | 3505 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Fagales › Betulaceae › Betula |
Subcellular locationi
Chloroplast
- chloroplast By similarity
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 22 | ChloroplastSequence analysisAdd BLAST | 22 | |
ChainiPRO_0000019704 | 23 – 583 | Ferredoxin--nitrite reductase, chloroplasticAdd BLAST | 561 |
Proteomic databases
PRIDEi | P38500 |
Expressioni
Inductioni
By nitrate.
Interactioni
Subunit structurei
Monomer.
Family & Domainsi
Sequence similaritiesi
Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.Curated
Keywords - Domaini
Transit peptideFamily and domain databases
InterProi | View protein in InterPro IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR006067, NO2/SO3_Rdtase_4Fe4S_dom IPR006066, NO2/SO3_Rdtase_FeS/sirohaem_BS |
Pfami | View protein in Pfam PF01077, NIR_SIR, 2 hits PF03460, NIR_SIR_ferr, 2 hits |
PRINTSi | PR00397, SIROHAEM |
SUPFAMi | SSF55124, SSF55124, 2 hits |
PROSITEi | View protein in PROSITE PS00365, NIR_SIR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P38500-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSLSVRFLS PPLFSSTPAW PRTGLAATQA VPPVVAEVDA GRLEPRVEER
60 70 80 90 100
EGYWVLKEKF REGINPQEKL KLEREPMKLF MEGGIEDLAK MSLEEIDKDK
110 120 130 140 150
ISKSDIDVRL KWLGLFHRRK HHYGRFMMRL KLPNGVTTSA QTRYLASVIR
160 170 180 190 200
KYGKDGCADV TTRQNWQIRG VVLSDVPEIL KGLDEVGLTS LQSGMDNVRN
210 220 230 240 250
PVGNPLAGID IHEIVATRPY NNLLSQFITA NSRGNLAFTN LPRKWNVCVV
260 270 280 290 300
GSHDLFEHPH INDLAYMPAI KDGRFGFNLL VGGFFSPRRC AEAVPLDAWV
310 320 330 340 350
SADDIILVCK AILEAYRDLG TRGNRQKTRM MWLIDELGIE GFRSEVVKRM
360 370 380 390 400
PNQELERAAP EDLIEKQWER RELIGVHPQK QEGLSYVGLH IPVGRVQADD
410 420 430 440 450
MDELARLADT YGCGELRLTV EQNIIIPNIE NSKLEALLGE PLLKDRFSPE
460 470 480 490 500
PPILMKGLVA CTGNQFCGQA IIETKARALK VTEEVQRQVA VTRPVRMHWT
510 520 530 540 550
GCPNSCGQVQ VADIGFMGCM ARDENGKPCE GAAVFLGGRI GSDSHLGNLY
560 570 580
KKGVPCKNLV PLVVDILVKH FGAVPREREE SED
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60093 mRNA Translation: CAA42690.1 |
PIRi | S20495 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X60093 mRNA Translation: CAA42690.1 |
PIRi | S20495 |
3D structure databases
SMRi | P38500 |
ModBasei | Search... |
Proteomic databases
PRIDEi | P38500 |
Enzyme and pathway databases
UniPathwayi | UPA00653 |
Family and domain databases
InterProi | View protein in InterPro IPR005117, NiRdtase/SiRdtase_haem-b_fer IPR036136, Nit/Sulf_reduc_fer-like_dom_sf IPR006067, NO2/SO3_Rdtase_4Fe4S_dom IPR006066, NO2/SO3_Rdtase_FeS/sirohaem_BS |
Pfami | View protein in Pfam PF01077, NIR_SIR, 2 hits PF03460, NIR_SIR_ferr, 2 hits |
PRINTSi | PR00397, SIROHAEM |
SUPFAMi | SSF55124, SSF55124, 2 hits |
PROSITEi | View protein in PROSITE PS00365, NIR_SIR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NIR_BETPN | |
Accessioni | P38500Primary (citable) accession number: P38500 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | October 7, 2020 | |
This is version 101 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families