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UniProtKB - P38489 (NFSB_ECOLI)
Protein
Oxygen-insensitive NAD(P)H nitroreductase
Gene
nfsB
Organism
Escherichia coli (strain K12)
Status
Functioni
Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.
1 PublicationCatalytic activityi
Cofactori
Activity regulationi
Subject to competitive inhibition by dicoumarol, nicotinic acid and acetate with respect to NADH. Subject to uncompetitive inhibition by dicoumarol, nicotinic acid and acetate with respect to nitrofurazone and nitrofurantoin.1 Publication
Kineticsi
- KM=350 µM for NADH1 Publication
- KM=1850 µM for nitrofurazone1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 14 | NADBy similarity | 1 | |
Binding sitei | 41 | NAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 71 | FMN3 Publications | 1 | |
Binding sitei | 71 | NADBy similarity | 1 | |
Binding sitei | 74 | NADBy similarity | 1 | |
Binding sitei | 107 | NAD; via carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 14 | FMN3 Publications | 5 | |
Nucleotide bindingi | 165 – 166 | FMN3 Publications | 2 | |
Nucleotide bindingi | 205 – 207 | FMN3 Publications | 3 |
GO - Molecular functioni
- 6,7-dihydropteridine reductase activity Source: EcoCyc
- FMN binding Source: EcoCyc
- identical protein binding Source: IntAct
- NAD(P)H nitroreductase activity Source: EcoCyc
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- 2,4,6-trinitrotoluene catabolic process Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Flavoprotein, FMN, NAD, NADP |
Enzyme and pathway databases
BioCyci | EcoCyc:DIHYDROPTERIREDUCT-MONOMER |
BRENDAi | 1.7.1.B2, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:nfsB1 Publication Synonyms:dprA, nfnB1 Publication, nfsI, ntr Ordered Locus Names:b0578, JW0567 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL1075080 |
DrugBanki | DB04138, 5-[Bis(2-bromoethyl)amino]-N-(2,3-dioxopropyl)-2,4-dinitrobenzamide DB03228, 5-[Bis-2(Chloro-Ethyl)-Amino]-2,4-Dintro-Benzamide DB04392, Bishydroxy[2h-1-Benzopyran-2-One,1,2-Benzopyrone] DB03247, Flavin mononucleotide DB04253, Tretazicar |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000072711 | 1 – 217 | Oxygen-insensitive NAD(P)H nitroreductaseAdd BLAST | 217 |
Proteomic databases
jPOSTi | P38489 |
PaxDbi | P38489 |
PRIDEi | P38489 |
2D gel databases
SWISS-2DPAGEi | P38489 |
Interactioni
Subunit structurei
Homodimer.
4 PublicationsBinary interactionsi
P38489
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-909296,EBI-909296 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262913, 20 interactors 850145, 1 interactor |
DIPi | DIP-10330N |
IntActi | P38489, 12 interactors |
STRINGi | 511145.b0578 |
Chemistry databases
BindingDBi | P38489 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P38489 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P38489 |
Family & Domainsi
Sequence similaritiesi
Belongs to the nitroreductase family.Curated
Phylogenomic databases
eggNOGi | COG0778, Bacteria |
HOGENOMi | CLU_070764_4_1_6 |
InParanoidi | P38489 |
PhylomeDBi | P38489 |
Family and domain databases
CDDi | cd02149, NfsB-like, 1 hit |
Gene3Di | 3.40.109.10, 1 hit |
InterProi | View protein in InterPro IPR033878, NfsB-like IPR029479, Nitroreductase IPR000415, Nitroreductase-like |
Pfami | View protein in Pfam PF00881, Nitroreductase, 1 hit |
SUPFAMi | SSF55469, SSF55469, 1 hit |
i Sequence
Sequence statusi: Complete.
P38489-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV
60 70 80 90 100
ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD
110 120 130 140 150
QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF
160 170 180 190 200
LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN
210
ATLPKSRLPQ NITLTEV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | S → C AA sequence (PubMed:3060113).Curated | 1 | |
Sequence conflicti | 10 – 12 | RHS → CIV AA sequence (PubMed:3060113).Curated | 3 | |
Sequence conflicti | 19 | S → M AA sequence (PubMed:3060113).Curated | 1 | |
Sequence conflicti | 21 | Missing AA sequence (PubMed:1472094).Curated | 1 | |
Sequence conflicti | 28 | E → D AA sequence (PubMed:1472094).Curated | 1 | |
Sequence conflicti | 180 | E → I AA sequence (PubMed:1472094).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D25414 Genomic DNA Translation: BAA05004.1 U07860 Genomic DNA Translation: AAC43263.1 U82598 Genomic DNA Translation: AAB40776.1 U00096 Genomic DNA Translation: AAC73679.1 AP009048 Genomic DNA Translation: BAA35218.1 |
PIRi | I67685 S01818 |
RefSeqi | NP_415110.1, NC_000913.3 WP_000351487.1, NZ_SSZK01000024.1 |
Genome annotation databases
EnsemblBacteriai | AAC73679; AAC73679; b0578 BAA35218; BAA35218; BAA35218 |
GeneIDi | 945778 |
KEGGi | ecj:JW0567 eco:b0578 |
PATRICi | fig|1411691.4.peg.1696 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D25414 Genomic DNA Translation: BAA05004.1 U07860 Genomic DNA Translation: AAC43263.1 U82598 Genomic DNA Translation: AAB40776.1 U00096 Genomic DNA Translation: AAC73679.1 AP009048 Genomic DNA Translation: BAA35218.1 |
PIRi | I67685 S01818 |
RefSeqi | NP_415110.1, NC_000913.3 WP_000351487.1, NZ_SSZK01000024.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DS7 | X-ray | 2.06 | A/B | 1-217 | [»] | |
1ICR | X-ray | 1.70 | A/B | 1-217 | [»] | |
1ICU | X-ray | 1.80 | A/B/C/D | 1-217 | [»] | |
1ICV | X-ray | 2.40 | A/B/C/D | 1-217 | [»] | |
1IDT | X-ray | 2.00 | A/B | 1-217 | [»] | |
1OO5 | X-ray | 2.50 | A/B | 1-217 | [»] | |
1OO6 | X-ray | 2.00 | A/B | 1-217 | [»] | |
1OON | X-ray | 2.49 | A/B | 1-217 | [»] | |
1OOQ | X-ray | 2.00 | A/B | 1-217 | [»] | |
1YKI | X-ray | 1.70 | A/B/C/D | 1-217 | [»] | |
1YLR | X-ray | 1.70 | A/B | 1-217 | [»] | |
1YLU | X-ray | 2.00 | A/B | 1-217 | [»] | |
3X21 | X-ray | 3.00 | A/B/C/D/E/F/G/H/I/J | 1-217 | [»] | |
3X22 | X-ray | 2.00 | A/B | 1-217 | [»] | |
SMRi | P38489 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262913, 20 interactors 850145, 1 interactor |
DIPi | DIP-10330N |
IntActi | P38489, 12 interactors |
STRINGi | 511145.b0578 |
Chemistry databases
BindingDBi | P38489 |
ChEMBLi | CHEMBL1075080 |
DrugBanki | DB04138, 5-[Bis(2-bromoethyl)amino]-N-(2,3-dioxopropyl)-2,4-dinitrobenzamide DB03228, 5-[Bis-2(Chloro-Ethyl)-Amino]-2,4-Dintro-Benzamide DB04392, Bishydroxy[2h-1-Benzopyran-2-One,1,2-Benzopyrone] DB03247, Flavin mononucleotide DB04253, Tretazicar |
2D gel databases
SWISS-2DPAGEi | P38489 |
Proteomic databases
jPOSTi | P38489 |
PaxDbi | P38489 |
PRIDEi | P38489 |
Genome annotation databases
EnsemblBacteriai | AAC73679; AAC73679; b0578 BAA35218; BAA35218; BAA35218 |
GeneIDi | 945778 |
KEGGi | ecj:JW0567 eco:b0578 |
PATRICi | fig|1411691.4.peg.1696 |
Organism-specific databases
EchoBASEi | EB4146 |
Phylogenomic databases
eggNOGi | COG0778, Bacteria |
HOGENOMi | CLU_070764_4_1_6 |
InParanoidi | P38489 |
PhylomeDBi | P38489 |
Enzyme and pathway databases
BioCyci | EcoCyc:DIHYDROPTERIREDUCT-MONOMER |
BRENDAi | 1.7.1.B2, 2026 |
Miscellaneous databases
EvolutionaryTracei | P38489 |
PROi | PR:P38489 |
Family and domain databases
CDDi | cd02149, NfsB-like, 1 hit |
Gene3Di | 3.40.109.10, 1 hit |
InterProi | View protein in InterPro IPR033878, NfsB-like IPR029479, Nitroreductase IPR000415, Nitroreductase-like |
Pfami | View protein in Pfam PF00881, Nitroreductase, 1 hit |
SUPFAMi | SSF55469, SSF55469, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NFSB_ECOLI | |
Accessioni | P38489Primary (citable) accession number: P38489 Secondary accession number(s): P19575 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families