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UniProtKB - P38433 (ACE1_CAEEL)
Protein
Acetylcholinesterase 1
Gene
ace-1
Organism
Caenorhabditis elegans
Status
Functioni
Rapidly hydrolyzes acetylcholine and releases choline into the synapse (Probable). It can hydrolyze propionylcholine and butyrylthiocholine in vitro (Probable).
1 PublicationCatalytic activityi
Kineticsi
- KM=57 µM for acetylthiocholine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 216 | Acyl-ester intermediatePROSITE-ProRule annotation | 1 | |
Active sitei | 346 | Charge relay systemBy similarity | 1 | |
Active sitei | 468 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- acetylcholinesterase activity Source: WormBase
- carboxylic ester hydrolase activity Source: GO_Central
- identical protein binding Source: WormBase
GO - Biological processi
- acetylcholine catabolic process Source: WormBase
- acetylcholine catabolic process in synaptic cleft Source: WormBase
- choline metabolic process Source: GO_Central
- regulation of locomotion Source: WormBase
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Biological process | Neurotransmitter degradation |
Protein family/group databases
ESTHERi | caeel-ACHE1, AChE |
MEROPSi | S09.979 |
Names & Taxonomyi
Protein namesi | Recommended name: Acetylcholinesterase 1 (EC:3.1.1.71 Publication)Short name: ACE-11 Publication Short name: AChE 1 |
Gene namesi | Name:ace-1 ORF Names:W09B12.1 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | W09B12.1 ; CE07569 ; WBGene00000035 ; ace-1 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Extracellular region or secreted
- Secreted By similarity
Other locations
- synapse By similarity
Note: May be secreted or membrane associated via a non-catalytic subunit.
Extracellular region or secreted
- extracellular space Source: WormBase
- synaptic cleft Source: GOC
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- synapse Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Secreted, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Sequence analysisAdd BLAST | 31 | |
ChainiPRO_0000008610 | 32 – 620 | Acetylcholinesterase 1Add BLAST | 589 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 74 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 82 ↔ 109 | By similarity | ||
Disulfide bondi | 270 ↔ 286 | By similarity | ||
Glycosylationi | 272 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 430 ↔ 558 | By similarity | ||
Glycosylationi | 486 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 536 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 618 | InterchainBy similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | P38433 |
PaxDbi | P38433 |
PeptideAtlasi | P38433 |
PTM databases
iPTMneti | P38433 |
Expressioni
Developmental stagei
Detected at all stages. Found to be more abundant in larval stages than in embryos or adults.
Gene expression databases
Bgeei | WBGene00000035, Expressed in multi-cellular organism and 4 other tissues |
Interactioni
Subunit structurei
Oligomer composed of disulfide-linked homodimers.
By similarityGO - Molecular functioni
- identical protein binding Source: WormBase
Protein-protein interaction databases
BioGRIDi | 46591, 1 interactor |
STRINGi | 6239.W09B12.1.2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the type-B carboxylesterase/lipase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4389, Eukaryota |
GeneTreei | ENSGT00940000159300 |
HOGENOMi | CLU_006586_13_0_1 |
InParanoidi | P38433 |
OMAi | CPGQETA |
OrthoDBi | 754103at2759 |
PhylomeDBi | P38433 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS IPR000997, Cholinesterase |
Pfami | View protein in Pfam PF00135, COesterase, 1 hit |
PRINTSi | PR00878, CHOLNESTRASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P38433-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRNSLLFFIF LPSTILAVDL IHLHDGSPLF GEEVLSQTGK PLTRFQGIPF
60 70 80 90 100
AEPPVGNLRF KKPKPKQPWR IPLNATTPPN SCIQSEDTYF GDFYGSTMWN
110 120 130 140 150
ANTKLSEDCL YLNVYVPGKV DPNKKLAVMV WVYGGGFWSG TATLDVYDGR
160 170 180 190 200
ILTVEENVIL VAMNYRVSIF GFLYMNRPEA PGNMGMWDQL LAMKWVHKNI
210 220 230 240 250
DLFGGDLSRI TLFGESAGAA SVSIHMLSPK SAPYFHRAII QSGSATSPWA
260 270 280 290 300
IEPRDVALAR AVILYNAMKC GNMSLINPDY DRILDCFQRA DADALRENEW
310 320 330 340 350
APVREFGDFP WVPVVDGDFL LENAQTSLKQ GNFKKTQLLA GSNRDESIYF
360 370 380 390 400
LTYQLPDIFP VADFFTKTDF IKDRQLWIKG VKDLLPRQIL KCQLTLAAVL
410 420 430 440 450
HEYEPQDLPV TPRDWINAMD KMLGDYHFTC SVNEMALAHT KHGGDTYYYY
460 470 480 490 500
FTHRASQQTW PEWMGVLHGY EINFIFGEPL NQKRFNYTDE ERELSNRFMR
510 520 530 540 550
YWANFAKTGD PNKNEDGSFT QDVWPKYNSV SMEYMNMTVE SSYPSMKRIG
560 570 580 590 600
HGPRRKECAF WKAYLPNLMA AVADVGDPYL VWKQQMDKWQ NEYITDWQYH
610 620
FEQYKRYQTY RQSDSETCGG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75331 mRNA Translation: CAA53080.1 FO081067 Genomic DNA Translation: CCD68912.1 |
PIRi | A54413 T29347 |
RefSeqi | NP_510660.1, NM_078259.6 |
Genome annotation databases
EnsemblMetazoai | W09B12.1.1; W09B12.1.1; WBGene00000035 W09B12.1.2; W09B12.1.2; WBGene00000035 |
GeneIDi | 181706 |
KEGGi | cel:CELE_W09B12.1 |
UCSCi | W09B12.1.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X75331 mRNA Translation: CAA53080.1 FO081067 Genomic DNA Translation: CCD68912.1 |
PIRi | A54413 T29347 |
RefSeqi | NP_510660.1, NM_078259.6 |
3D structure databases
AlphaFoldDBi | P38433 |
SMRi | P38433 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 46591, 1 interactor |
STRINGi | 6239.W09B12.1.2 |
Chemistry databases
ChEMBLi | CHEMBL3341583 |
Protein family/group databases
ESTHERi | caeel-ACHE1, AChE |
MEROPSi | S09.979 |
PTM databases
iPTMneti | P38433 |
Proteomic databases
EPDi | P38433 |
PaxDbi | P38433 |
PeptideAtlasi | P38433 |
Genome annotation databases
EnsemblMetazoai | W09B12.1.1; W09B12.1.1; WBGene00000035 W09B12.1.2; W09B12.1.2; WBGene00000035 |
GeneIDi | 181706 |
KEGGi | cel:CELE_W09B12.1 |
UCSCi | W09B12.1.1, c. elegans |
Organism-specific databases
CTDi | 181706 |
WormBasei | W09B12.1 ; CE07569 ; WBGene00000035 ; ace-1 |
Phylogenomic databases
eggNOGi | KOG4389, Eukaryota |
GeneTreei | ENSGT00940000159300 |
HOGENOMi | CLU_006586_13_0_1 |
InParanoidi | P38433 |
OMAi | CPGQETA |
OrthoDBi | 754103at2759 |
PhylomeDBi | P38433 |
Miscellaneous databases
PROi | PR:P38433 |
Gene expression databases
Bgeei | WBGene00000035, Expressed in multi-cellular organism and 4 other tissues |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS IPR000997, Cholinesterase |
Pfami | View protein in Pfam PF00135, COesterase, 1 hit |
PRINTSi | PR00878, CHOLNESTRASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACE1_CAEEL | |
Accessioni | P38433Primary (citable) accession number: P38433 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | May 25, 2022 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families