UniProtKB - P38422 (DACF_BACSU)
Protein
D-alanyl-D-alanine carboxypeptidase DacF
Gene
dacF
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.
Catalytic activityi
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine. EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 64 | Acyl-ester intermediateBy similarity | 1 | |
Active sitei | 67 | Proton acceptorBy similarity | 1 | |
Active sitei | 124 | By similarity | 1 | |
Binding sitei | 230 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- endopeptidase activity Source: GO_Central
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: UniProtKB-EC
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU23480-MONOMER |
UniPathwayi | UPA00219 |
Protein family/group databases
MEROPSi | S11.005 |
Names & Taxonomyi
Protein namesi | Recommended name: D-alanyl-D-alanine carboxypeptidase DacF (EC:3.4.16.4)Short name: DD-carboxypeptidase Short name: DD-peptidase Alternative name(s): Penicillin-binding protein DacF Short name: PBP |
Gene namesi | Name:dacF Ordered Locus Names:BSU23480 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000027230 | 24 – 389 | D-alanyl-D-alanine carboxypeptidase DacFAdd BLAST | 366 |
Proteomic databases
PaxDbi | P38422 |
PRIDEi | P38422 |
Expressioni
Developmental stagei
Expressed in the forespore.
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S11 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1686, Bacteria |
InParanoidi | P38422 |
OMAi | AYSDYIV |
PhylomeDBi | P38422 |
Family and domain databases
Gene3Di | 2.60.410.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR015956, Peniciliin-bd_prot_C_sf IPR018044, Peptidase_S11 IPR012907, Peptidase_S11_C IPR037167, Peptidase_S11_C_sf IPR001967, Peptidase_S11_N |
Pfami | View protein in Pfam PF07943, PBP5_C, 1 hit PF00768, Peptidase_S11, 1 hit |
PRINTSi | PR00725, DADACBPTASE1 |
SMARTi | View protein in SMART SM00936, PBP5_C, 1 hit |
SUPFAMi | SSF56601, SSF56601, 1 hit SSF69189, SSF69189, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P38422-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRLLSTLLI GIMLLTFAPS AFAKQDGKRT SELAHEAKSA VLIERDTGKV
60 70 80 90 100
LYNKNSNERL APASMTKIMT MLLIMEALDK GKIKMSDKVR TSEHAASMGG
110 120 130 140 150
SQIFLEPGEE MTVKEMLKGI AIASGNDASV AMAEFISGSE EEFVKKMNKK
160 170 180 190 200
AKELGLKNTS FKNPTGLTEE GHYSSAYDMA IMAKELLKYE SITKFTGTYE
210 220 230 240 250
DYLRENTDKK FWLVNTNRLI KFYPGVDGVK TGYTGEAKYC LTASAKKGNM
260 270 280 290 300
RAIAVVFGAS TPKERNAQVT KMLDFAFSQY ETHPLYKRNQ TVAKVKVKKG
310 320 330 340 350
KQKFIELTTS EPISILTKKG EDMNDVKKEI KMKDNISAPI QKGQELGTLV
360 370 380
LKKDGEVLAE SPVAAKEDMK KAGFITFLKR TMGDWTKFK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 376 | T → S in AAA22704 (PubMed:1629150).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M85047 Genomic DNA Translation: AAA22704.1 D84432 Genomic DNA Translation: BAA12652.1 AL009126 Genomic DNA Translation: CAB14280.1 |
PIRi | B42708 |
RefSeqi | NP_390229.1, NC_000964.3 WP_004398637.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14280; CAB14280; BSU_23480 |
GeneIDi | 938732 |
KEGGi | bsu:BSU23480 |
PATRICi | fig|224308.179.peg.2558 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M85047 Genomic DNA Translation: AAA22704.1 D84432 Genomic DNA Translation: BAA12652.1 AL009126 Genomic DNA Translation: CAB14280.1 |
PIRi | B42708 |
RefSeqi | NP_390229.1, NC_000964.3 WP_004398637.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | P38422 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU23480 |
Protein family/group databases
MEROPSi | S11.005 |
Proteomic databases
PaxDbi | P38422 |
PRIDEi | P38422 |
Genome annotation databases
EnsemblBacteriai | CAB14280; CAB14280; BSU_23480 |
GeneIDi | 938732 |
KEGGi | bsu:BSU23480 |
PATRICi | fig|224308.179.peg.2558 |
Phylogenomic databases
eggNOGi | COG1686, Bacteria |
InParanoidi | P38422 |
OMAi | AYSDYIV |
PhylomeDBi | P38422 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | BSUB:BSU23480-MONOMER |
Family and domain databases
Gene3Di | 2.60.410.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR015956, Peniciliin-bd_prot_C_sf IPR018044, Peptidase_S11 IPR012907, Peptidase_S11_C IPR037167, Peptidase_S11_C_sf IPR001967, Peptidase_S11_N |
Pfami | View protein in Pfam PF07943, PBP5_C, 1 hit PF00768, Peptidase_S11, 1 hit |
PRINTSi | PR00725, DADACBPTASE1 |
SMARTi | View protein in SMART SM00936, PBP5_C, 1 hit |
SUPFAMi | SSF56601, SSF56601, 1 hit SSF69189, SSF69189, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DACF_BACSU | |
Accessioni | P38422Primary (citable) accession number: P38422 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | August 29, 2003 | |
Last modified: | April 7, 2021 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families