UniProtKB - P38418 (LOX2_ARATH)
Lipoxygenase 2, chloroplastic
LOX2
Functioni
13S-lipoxygenase that can use linolenic acid as substrates. Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Required for the wound-induced synthesis of jasmonic acid (JA) in leaves.
2 PublicationsCatalytic activityi
- EC:1.13.11.121 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:1.13.11.121 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
: oxylipin biosynthesis Pathwayi
This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 554 | Iron; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 559 | Iron; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 746 | Iron; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 750 | Iron; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 896 | Iron; via carboxylate; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- linoleate 13S-lipoxygenase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- mRNA binding Source: TAIR
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Source: GO_Central
- salicylic acid binding Source: TAIR
GO - Biological processi
- fatty acid biosynthetic process Source: UniProtKB-KW
- green leaf volatile biosynthetic process Source: TAIR
- jasmonic acid biosynthetic process Source: TAIR
- lipid oxidation Source: TAIR
- oxylipin biosynthetic process Source: UniProtKB-UniPathway
- response to bacterium Source: UniProtKB
- response to fungus Source: TAIR
- response to herbivore Source: UniProtKB
- response to jasmonic acid Source: TAIR
- response to wounding Source: UniProtKB
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:AT3G45140-MONOMER |
BRENDAi | 1.13.11.12, 399 |
UniPathwayi | UPA00382 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:LOX2 Ordered Locus Names:At3g45140 ORF Names:T14D3.80 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G45140 |
TAIRi | locus:2096915, AT3G45140 |
Subcellular locationi
Chloroplast
- chloroplast 1 Publication
Cytoplasm and Cytosol
Note: The unprocessed form is cytoplasmic whereas the cleaved form is chloroplastic.
Chloroplast
- chloroplast Source: UniProtKB
- chloroplast envelope Source: TAIR
- chloroplast stroma Source: TAIR
- chloroplast thylakoid membrane Source: TAIR
Cytosol
- cytosol Source: TAIR
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Chloroplast, Cytoplasm, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 56 | ChloroplastSequence analysisAdd BLAST | 56 | |
ChainiPRO_0000018326 | 57 – 896 | Lipoxygenase 2, chloroplasticAdd BLAST | 840 |
Proteomic databases
PaxDbi | P38418 |
PRIDEi | P38418 |
ProteomicsDBi | 238417 |
2D gel databases
SWISS-2DPAGEi | P38418 |
PTM databases
iPTMneti | P38418 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
ExpressionAtlasi | P38418, baseline and differential |
Genevisiblei | P38418, AT |
Interactioni
Subunit structurei
Interacts with EIF4E2.
1 PublicationBinary interactionsi
P38418
With | #Exp. | IntAct |
---|---|---|
EIF(ISO)4E [O04663] | 7 | EBI-1770437,EBI-1770425 |
Protein-protein interaction databases
BioGRIDi | 8970, 5 interactors |
IntActi | P38418, 4 interactors |
STRINGi | 3702.AT3G45140.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 79 – 199 | PLATPROSITE-ProRule annotationAdd BLAST | 121 | |
Domaini | 202 – 896 | LipoxygenasePROSITE-ProRule annotationAdd BLAST | 695 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 175 – 232 | EIF4E2 bindingAdd BLAST | 58 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | ENOG502QQSP, Eukaryota |
HOGENOMi | CLU_004282_0_0_1 |
InParanoidi | P38418 |
OrthoDBi | 385042at2759 |
PhylomeDBi | P38418 |
Family and domain databases
CDDi | cd01751, PLAT_LH2, 1 hit |
Gene3Di | 4.10.372.10, 1 hit |
InterProi | View protein in InterPro IPR000907, LipOase IPR013819, LipOase_C IPR036226, LipOase_C_sf IPR020834, LipOase_CS IPR020833, LipOase_Fe_BS IPR001246, LipOase_plant IPR042057, Lipoxy_PLAT/LH2 IPR027433, Lipoxygenase_dom_3 IPR001024, PLAT/LH2_dom IPR036392, PLAT/LH2_dom_sf |
PANTHERi | PTHR11771, PTHR11771, 2 hits |
Pfami | View protein in Pfam PF00305, Lipoxygenase, 1 hit PF01477, PLAT, 1 hit |
PRINTSi | PR00087, LIPOXYGENASE PR00468, PLTLPOXGNASE |
SMARTi | View protein in SMART SM00308, LH2, 1 hit |
SUPFAMi | SSF48484, SSF48484, 1 hit SSF49723, SSF49723, 1 hit |
PROSITEi | View protein in PROSITE PS00711, LIPOXYGENASE_1, 1 hit PS00081, LIPOXYGENASE_2, 1 hit PS51393, LIPOXYGENASE_3, 1 hit PS50095, PLAT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MYCRESLSSL QTLNVAKSLS SLFPKQSALI NPISAGRRNN LPRPNLRRRC
60 70 80 90 100
KVTASRANIE QEGNTVKEPI QNIKVKGYIT AQEEFLEGIT WSRGLDDIAD
110 120 130 140 150
IRGRSLLVEL ISAKTDQRIT VEDYAQRVWA EAPDEKYECE FEMPEDFGPV
160 170 180 190 200
GAIKIQNQYH RQLFLKGVEL KLPGGSITFT CESWVAPKSV DPTKRIFFSD
210 220 230 240 250
KSYLPSQTPE PLKKYRKEEL ETLQGKNREE VGEFTKFERI YDYDVYNDVG
260 270 280 290 300
DPDNDPELAR PVIGGLTHPY PRRCKTGRKP CETDPSSEQR YGGEFYVPRD
310 320 330 340 350
EEFSTAKGTS FTGKAVLAAL PSIFPQIESV LLSPQEPFPH FKAIQNLFEE
360 370 380 390 400
GIQLPKDAGL LPLLPRIIKA LGEAQDDILQ FDAPVLINRD RFSWLRDDEF
410 420 430 440 450
ARQTLAGLNP YSIQLVEEWP LISKLDPAVY GDPTSLITWE IVEREVKGNM
460 470 480 490 500
TVDEALKNKR LFVLDYHDLL LPYVNKVREL NNTTLYASRT LFFLSDDSTL
510 520 530 540 550
RPVAIELTCP PNINKPQWKQ VFTPGYDATS CWLWNLAKTH AISHDAGYHQ
560 570 580 590 600
LISHWLRTHA CTEPYIIAAN RQLSAMHPIY RLLHPHFRYT MEINARARQS
610 620 630 640 650
LVNGGGIIET CFWPGKYALE LSSAVYGKLW RFDQEGLPAD LIKRGLAEED
660 670 680 690 700
KTAEHGVRLT IPDYPFANDG LILWDAIKEW VTDYVKHYYP DEELITSDEE
710 720 730 740 750
LQGWWSEVRN IGHGDKKDEP WWPVLKTQDD LIGVVTTIAW VTSGHHAAVN
760 770 780 790 800
FGQYGYGGYF PNRPTTTRIR MPTEDPTDEA LKEFYESPEK VLLKTYPSQK
810 820 830 840 850
QATLVMVTLD LLSTHSPDEE YIGEQQEASW ANEPVINAAF ERFKGKLQYL
860 870 880 890
EGVIDERNVN ITLKNRAGAG VVKYELLKPT SEHGVTGMGV PYSISI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1I9LPH1 | A0A1I9LPH1_ARATH | Lipoxygenase | LOX2 ATLOX2, lipoxygenase 2, At3g45140 | 754 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 119 – 125 | ITVEDYA → EYTFFFL in BAC43666 (PubMed:11910074).Curated | 7 | |
Sequence conflicti | 613 | W → C in AAL32689 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 646 | L → V in BAF01939 (Ref. 6) Curated | 1 | |
Sequence conflicti | 731 | L → F in BAF01939 (Ref. 6) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L23968 mRNA Translation: AAA32749.1 AL138649 Genomic DNA Translation: CAB72152.1 Sequence problems. CP002686 Genomic DNA Translation: AEE77997.1 AY062611 mRNA Translation: AAL32689.1 AK119093 mRNA Translation: BAC43666.1 AK222075 mRNA Translation: BAD94917.1 AK230124 mRNA Translation: BAF01939.1 |
PIRi | JQ2391 T47454 |
RefSeqi | NP_566875.1, NM_114383.3 |
Genome annotation databases
EnsemblPlantsi | AT3G45140.1; AT3G45140.1; AT3G45140 |
GeneIDi | 823650 |
Gramenei | AT3G45140.1; AT3G45140.1; AT3G45140 |
KEGGi | ath:AT3G45140 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L23968 mRNA Translation: AAA32749.1 AL138649 Genomic DNA Translation: CAB72152.1 Sequence problems. CP002686 Genomic DNA Translation: AEE77997.1 AY062611 mRNA Translation: AAL32689.1 AK119093 mRNA Translation: BAC43666.1 AK222075 mRNA Translation: BAD94917.1 AK230124 mRNA Translation: BAF01939.1 |
PIRi | JQ2391 T47454 |
RefSeqi | NP_566875.1, NM_114383.3 |
3D structure databases
SMRi | P38418 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 8970, 5 interactors |
IntActi | P38418, 4 interactors |
STRINGi | 3702.AT3G45140.1 |
PTM databases
iPTMneti | P38418 |
2D gel databases
SWISS-2DPAGEi | P38418 |
Proteomic databases
PaxDbi | P38418 |
PRIDEi | P38418 |
ProteomicsDBi | 238417 |
Genome annotation databases
EnsemblPlantsi | AT3G45140.1; AT3G45140.1; AT3G45140 |
GeneIDi | 823650 |
Gramenei | AT3G45140.1; AT3G45140.1; AT3G45140 |
KEGGi | ath:AT3G45140 |
Organism-specific databases
Araporti | AT3G45140 |
TAIRi | locus:2096915, AT3G45140 |
Phylogenomic databases
eggNOGi | ENOG502QQSP, Eukaryota |
HOGENOMi | CLU_004282_0_0_1 |
InParanoidi | P38418 |
OrthoDBi | 385042at2759 |
PhylomeDBi | P38418 |
Enzyme and pathway databases
UniPathwayi | UPA00382 |
BioCyci | ARA:AT3G45140-MONOMER |
BRENDAi | 1.13.11.12, 399 |
Miscellaneous databases
PROi | PR:P38418 |
Gene expression databases
ExpressionAtlasi | P38418, baseline and differential |
Genevisiblei | P38418, AT |
Family and domain databases
CDDi | cd01751, PLAT_LH2, 1 hit |
Gene3Di | 4.10.372.10, 1 hit |
InterProi | View protein in InterPro IPR000907, LipOase IPR013819, LipOase_C IPR036226, LipOase_C_sf IPR020834, LipOase_CS IPR020833, LipOase_Fe_BS IPR001246, LipOase_plant IPR042057, Lipoxy_PLAT/LH2 IPR027433, Lipoxygenase_dom_3 IPR001024, PLAT/LH2_dom IPR036392, PLAT/LH2_dom_sf |
PANTHERi | PTHR11771, PTHR11771, 2 hits |
Pfami | View protein in Pfam PF00305, Lipoxygenase, 1 hit PF01477, PLAT, 1 hit |
PRINTSi | PR00087, LIPOXYGENASE PR00468, PLTLPOXGNASE |
SMARTi | View protein in SMART SM00308, LH2, 1 hit |
SUPFAMi | SSF48484, SSF48484, 1 hit SSF49723, SSF49723, 1 hit |
PROSITEi | View protein in PROSITE PS00711, LIPOXYGENASE_1, 1 hit PS00081, LIPOXYGENASE_2, 1 hit PS51393, LIPOXYGENASE_3, 1 hit PS50095, PLAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LOX2_ARATH | |
Accessioni | P38418Primary (citable) accession number: P38418 Secondary accession number(s): Q0WLR8 Q9M1U5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families