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Protein

P-type cation-transporting ATPase

Gene

PCA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cadmium transporting P-type ATPase which plays a critical role in cadmium resistance by extruding intracellular cadmium. Capable of high affinity copper ion binding, but not active copper ion transport. May play a role in copper resistance by chelating and sequestering copper ions.7 Publications

Caution

Although initial characterizations described PCA1 as a copper-transporting ATPase, subsequent experiments have demonstrated that PCA1 is capable of high affinity copper ion binding, but not active copper ion transport.1 Publication
Strain S288C, as well as other laboratory cadmium-sensitive strains, contain a natural Gly-970-Arg mutation which eliminates cadmium transport function. Loss of cadmium resistance provides a fitness advantage under cadmium-free conditions.Curated

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi421CopperPROSITE-ProRule annotation1
Metal bindingi424CopperPROSITE-ProRule annotation1
Active sitei9034-aspartylphosphate intermediateBy similarity1
Metal bindingi1107MagnesiumPROSITE-ProRule annotation1
Metal bindingi1111MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cadmium-exporting ATPase activity Source: SGD
  • calcium-transporting ATPase activity Source: GO_Central
  • copper ion binding Source: SGD
  • proteasome binding Source: SGD

GO - Biological processi

  • cadmium ion transport Source: SGD
  • cellular calcium ion homeostasis Source: GO_Central
  • cellular iron ion homeostasis Source: SGD
  • cellular metal ion homeostasis Source: SGD
  • response to cadmium ion Source: UniProtKB-KW
  • transmembrane transport Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processCadmium resistance, Ion transport, Transport
LigandATP-binding, Cadmium, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29213-MONOMER

Protein family/group databases

TCDBi3.A.3.5.14 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
P-type cation-transporting ATPase (EC:3.6.3.3)
Alternative name(s):
Cadmium resistance protein 2
Cadmium-translocating P-type ATPase
Cd(2+)-exporting ATPase
Gene namesi
Name:PCA1
Synonyms:CAD2, PAY2
Ordered Locus Names:YBR295W
ORF Names:YBR2112
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR295W
SGDiS000000499 PCA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 556CytoplasmicSequence analysisAdd BLAST556
Transmembranei557 – 578HelicalSequence analysisAdd BLAST22
Topological domaini579 – 592ExtracellularSequence analysisAdd BLAST14
Transmembranei593 – 612HelicalSequence analysisAdd BLAST20
Topological domaini613 – 620CytoplasmicSequence analysis8
Transmembranei621 – 641HelicalSequence analysisAdd BLAST21
Topological domaini642 – 659ExtracellularSequence analysisAdd BLAST18
Transmembranei660 – 680HelicalSequence analysisAdd BLAST21
Topological domaini681 – 808CytoplasmicSequence analysisAdd BLAST128
Transmembranei809 – 831HelicalSequence analysisAdd BLAST23
Topological domaini832 – 847ExtracellularSequence analysisAdd BLAST16
Transmembranei848 – 865HelicalSequence analysisAdd BLAST18
Topological domaini866 – 1161CytoplasmicSequence analysisAdd BLAST296
Transmembranei1162 – 1181HelicalSequence analysisAdd BLAST20
Topological domaini1182 – 1190ExtracellularSequence analysis9
Transmembranei1191 – 1209HelicalSequence analysisAdd BLAST19
Topological domaini1210 – 1216CytoplasmicSequence analysis7

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi298C → A: Abolishes copper resistance but not cadmium resistance; when associated with A-300. 1 Publication1
Mutagenesisi300C → A: Abolishes copper resistance but not cadmium resistance; when associated with A-298. 1 Publication1
Mutagenesisi311C → A: Abolishes cadmium resistance yet retains the ability to confer copper resistance; when associated with A-312. 1 Publication1
Mutagenesisi312C → A: Abolishes cadmium resistance yet retains the ability to confer copper resistance; when associated with A-311. 1 Publication1
Mutagenesisi970R → G: Confers localization to the plasma membrane and cadmium transport function. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000463171 – 1216P-type cation-transporting ATPaseAdd BLAST1216

Post-translational modificationi

In the absence of cadmium, is ubiquitinated and targeted for degradation before reaching the plasma membrane. This allows a rapid and specific cellular response to cadmium.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiP38360
PRIDEiP38360

Interactioni

Protein-protein interaction databases

BioGridi32988, 61 interactors
DIPiDIP-6641N
IntActiP38360, 4 interactors
STRINGi4932.YBR295W

Structurei

3D structure databases

ProteinModelPortaliP38360
SMRiP38360
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini411 – 474HMAPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni250 – 350Metal-responding degradation signalAdd BLAST101

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000160566
InParanoidiP38360
KOiK01533
OMAiCTGCENK
OrthoDBiEOG092C0DN9

Family and domain databases

CDDicd00371 HMA, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006121 HMA_dom
IPR036163 HMA_dom_sf
IPR027256 P-typ_ATPase_IB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00403 HMA, 1 hit
SUPFAMiSSF55008 SSF55008, 1 hit
SSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 2 hits
TIGRFAMsiTIGR01525 ATPase-IB_hvy, 1 hit
TIGR01494 ATPase_P-type, 1 hit
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
PS50846 HMA_2, 1 hit

Sequencei

Sequence statusi: Complete.

P38360-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPEKLFSGL GTSDGEYGVV NSENISIDAM QDNRGECHRR SIEMHANDNL
60 70 80 90 100
GLVSQRDCTN RPKITPQECL SETEQICHHG ENRTKAGLDV DDAETGGDHT
110 120 130 140 150
NESRVDECCA EKVNDTETGL DVDSCCGDAQ TGGDHTNESC VDGCCVRDSS
160 170 180 190 200
VMVEEVTGSC EAVSSKEQLL TSFEVVPSKS EGLQSIHDIR ETTRCNTNSN
210 220 230 240 250
QHTGKGRLCI ESSDSTLKKR SCKVSRQKIE VSSKPECCNI SCVERIASRS
260 270 280 290 300
CEKRTFKGST NVGISGSSST DSLSEKFFSE QYSRMYNRYS SILKNLGCIC
310 320 330 340 350
NYLRTLGKES CCLPKVRFCS GEGASKKTKY SYRNSSGCLT KKKTHGDKER
360 370 380 390 400
LSNDNGHADF VCSKSCCTKM KDCAVTSTIS GHSSSEISRI VSMEPIENHL
410 420 430 440 450
NLEAGSTGTE HIVLSVSGMS CTGCESKLKK SFGALKCVHG LKTSLILSQA
460 470 480 490 500
EFNLDLAQGS VKDVIKHLSK TTEFKYEQIS NHGSTIDVVV PYAAKDFINE
510 520 530 540 550
EWPQGVTELK IVERNIIRIY FDPKVIGARD LVNEGWSVPV SIAPFSCHPT
560 570 580 590 600
IEVGRKHLVR VGCTTALSII LTIPILVMAW APQLREKIST ISASMVLATI
610 620 630 640 650
IQFVIAGPFY LNALKSLIFS RLIEMDLLIV LSTSAAYIFS IVSFGYFVVG
660 670 680 690 700
RPLSTEQFFE TSSLLVTLIM VGRFVSELAR HRAVKSISVR SLQASSAILV
710 720 730 740 750
DKTGKETEIN IRLLQYGDIF KVLPDSRIPT DGTVISGSSE VDEALITGES
760 770 780 790 800
MPVPKKCQSI VVAGSVNGTG TLFVKLSKLP GNNTISTIAT MVDEAKLTKP
810 820 830 840 850
KIQNIADKIA SYFVPTIIGI TVVTFCVWIA VGIRVEKQSR SDAVIQAIIY
860 870 880 890 900
AITVLIVSCP CVIGLAVPIV FVIASGVAAK RGVIFKSAES IEVAHNTSHV
910 920 930 940 950
VFDKTGTLTE GKLTVVHETV RGDRHNSQSL LLGLTEGIKH PVSMAIASYL
960 970 980 990 1000
KEKGVSAQNV SNTKAVTGKR VEGTSYSGLK LQGGNCRWLG HNNDPDVRKA
1010 1020 1030 1040 1050
LEQGYSVFCF SVNGSVTAVY ALEDSLRADA VSTINLLRQR GISLHILSGD
1060 1070 1080 1090 1100
DDGAVRSMAA RLGIESSNIR SHATPAEKSE YIKDIVEGRN CDSSSQSKRP
1110 1120 1130 1140 1150
VVVFCGDGTN DAIGLTQATI GVHINEGSEV AKLAADVVML KPKLNNILTM
1160 1170 1180 1190 1200
ITVSQKAMFR VKLNFLWSFT YNLFAILLAA GAFVDFHIPP EYAGLGELVS
1210
ILPVIFVAIL LRYAKI
Length:1,216
Mass (Da):131,875
Last modified:July 27, 2011 - v2
Checksum:iE4DCAFB23AA1F138
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti382H → T in CAA82529 (PubMed:7754711).Curated1
Sequence conflicti382H → T in CAA85260 (PubMed:7813418).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29332 Genomic DNA Translation: CAA82529.1
Z36164 Genomic DNA Translation: CAA85260.1
BK006936 Genomic DNA Translation: DAA07409.2
PIRiS46177
RefSeqiNP_009854.2, NM_001178643.2

Genome annotation databases

EnsemblFungiiYBR295W; YBR295W; YBR295W
GeneIDi852598
KEGGisce:YBR295W

Similar proteinsi

Entry informationi

Entry nameiATU1_YEAST
AccessioniPrimary (citable) accession number: P38360
Secondary accession number(s): D6VQT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: July 18, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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