UniProtKB - P38358 (VBA2_YEAST)
Protein
Vacuolar basic amino acid transporter 2
Gene
VBA2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Transporter required for vacuolar uptake of histidine, arginine and lysine and to a lesser extent tyrosine.1 Publication
GO - Molecular functioni
- basic amino acid transmembrane transporter activity Source: SGD
- transmembrane transporter activity Source: GO_Central
GO - Biological processi
- basic amino acid transport Source: SGD
- transmembrane transport Source: SGD
Keywordsi
Biological process | Amino-acid transport, Transport |
Protein family/group databases
TCDBi | 2.A.1.48.2, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar basic amino acid transporter 2 |
Gene namesi | Name:VBA2 Ordered Locus Names:YBR293W ORF Names:YBR2109 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000497, VBA2 |
VEuPathDBi | FungiDB:YBR293W |
Subcellular locationi
Vacuole
- Vacuole membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Vacuole
- fungal-type vacuole membrane Source: SGD
Other locations
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 33 | CytoplasmicSequence analysisAdd BLAST | 33 | |
Transmembranei | 34 – 54 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 55 – 62 | VacuolarSequence analysis | 8 | |
Transmembranei | 63 – 85 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 86 – 97 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 98 – 118 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 119 – 121 | VacuolarSequence analysis | 3 | |
Transmembranei | 122 – 142 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 143 – 167 | CytoplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 168 – 188 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 189 – 196 | VacuolarSequence analysis | 8 | |
Transmembranei | 197 – 217 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 218 – 238 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 239 – 259 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 260 – 273 | VacuolarSequence analysisAdd BLAST | 14 | |
Transmembranei | 274 – 294 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 295 – 303 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 304 – 324 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 325 – 331 | VacuolarSequence analysis | 7 | |
Transmembranei | 332 – 352 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 353 – 375 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 376 – 396 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 397 – 447 | VacuolarSequence analysisAdd BLAST | 51 | |
Transmembranei | 448 – 468 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 469 – 474 | CytoplasmicSequence analysis | 6 |
Keywords - Cellular componenti
Membrane, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173423 | 1 – 474 | Vacuolar basic amino acid transporter 2Add BLAST | 474 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 420 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | P38358 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 32986, 53 interactors |
DIPi | DIP-5142N |
IntActi | P38358, 4 interactors |
MINTi | P38358 |
STRINGi | 4932.YBR293W |
Miscellaneous databases
RNActi | P38358, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the major facilitator superfamily.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0254, Eukaryota |
HOGENOMi | CLU_000960_22_3_1 |
InParanoidi | P38358 |
OMAi | GMYQALQ |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P38358-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSISNWITTA YLITSTSFQP LYGSFSDALG RRNCLFFANG AFTIGCLACG
60 70 80 90 100
FSKNIYMLSF MRALTGIGGG GLITLSTIVN SDVIPSSKRG IFQAFQNLLL
110 120 130 140 150
GFGAICGASF GGTIASSIGW RWCFLIQVPI SVISSILMNY YVPNQKEYNR
160 170 180 190 200
QNSSIFQNPG KILRDIDVMG SILIITGLTL QLLYLSLGCS TSKLSWTSPS
210 220 230 240 250
VLLLLVGSVI ILLLFILHER KTSARAIIPM ELVNSSYSVV VLSISILVGF
260 270 280 290 300
ASYAYLFTLP LFFQIVLGDS TAKAGLRLTI PSLFTPVGSL ITGFSMSKYN
310 320 330 340 350
CLRLLLYIGI SLMFLGNFLF LFIEKTSPNW LIGLFLIPAN LGQGITFPTT
360 370 380 390 400
LFTFIFMFSK SDQATATSTL YLFRSIGSVW GVAISAGVIQ LSFAGLLRSN
410 420 430 440 450
LKGLLDENKI KKLIVQLSAN SSYIGSLHGE VKNTVIKSFD EATKRAHLMS
460 470
TLLSSLALIL CILKDNLAKP KTRR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36162 Genomic DNA Translation: CAA85258.1 AY692630 Genomic DNA Translation: AAT92649.1 BK006936 Genomic DNA Translation: DAA07407.1 |
PIRi | S46175 |
RefSeqi | NP_009852.3, NM_001178641.3 |
Genome annotation databases
EnsemblFungii | YBR293W_mRNA; YBR293W; YBR293W |
GeneIDi | 852596 |
KEGGi | sce:YBR293W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36162 Genomic DNA Translation: CAA85258.1 AY692630 Genomic DNA Translation: AAT92649.1 BK006936 Genomic DNA Translation: DAA07407.1 |
PIRi | S46175 |
RefSeqi | NP_009852.3, NM_001178641.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 32986, 53 interactors |
DIPi | DIP-5142N |
IntActi | P38358, 4 interactors |
MINTi | P38358 |
STRINGi | 4932.YBR293W |
Protein family/group databases
TCDBi | 2.A.1.48.2, the major facilitator superfamily (mfs) |
Proteomic databases
PaxDbi | P38358 |
Genome annotation databases
EnsemblFungii | YBR293W_mRNA; YBR293W; YBR293W |
GeneIDi | 852596 |
KEGGi | sce:YBR293W |
Organism-specific databases
SGDi | S000000497, VBA2 |
VEuPathDBi | FungiDB:YBR293W |
Phylogenomic databases
eggNOGi | KOG0254, Eukaryota |
HOGENOMi | CLU_000960_22_3_1 |
InParanoidi | P38358 |
OMAi | GMYQALQ |
Miscellaneous databases
PROi | PR:P38358 |
RNActi | P38358, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VBA2_YEAST | |
Accessioni | P38358Primary (citable) accession number: P38358 Secondary accession number(s): D6VQT7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | February 10, 2021 | |
This is version 154 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - SIMILARITY comments
Index of protein domains and families - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names