UniProtKB - P38352 (SAF1_YEAST)
Protein
SCF-associated factor 1
Gene
SAF1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Targets AAH1 adenine deaminase for proteasome-dependent degradation upon entry into quiescence. Targets also URA7.2 Publications
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Biological processi
- protein ubiquitination Source: UniProtKB-UniPathway
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: SCF-associated factor 1 |
Gene namesi | Name:SAF1 Ordered Locus Names:YBR280C ORF Names:YBR2017 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YBR280C |
SGDi | S000000484, SAF1 |
Subcellular locationi
Other locations
- SCF ubiquitin ligase complex Source: SGD
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 | L → P in saf1-5; impairs the interaction with SKP1. 1 Publication | 1 | |
Mutagenesisi | 35 | L → P in saf1-6; impairs the interaction with SKP1. 1 Publication | 1 | |
Mutagenesisi | 263 | R → K in saf1-4; impairs the protein stability; when associated with K-264. 1 Publication | 1 | |
Mutagenesisi | 264 | R → K in saf1-4; impairs the protein stability; when associated with K-263. 1 Publication | 1 | |
Mutagenesisi | 372 | G → V in saf1-1; impairs the protein stability. 1 Publication | 1 | |
Mutagenesisi | 387 | S → F in saf1-2; impairs the protein stability. 1 Publication | 1 | |
Mutagenesisi | 580 | L → S in saf1-3; impairs the protein stability. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206658 | 1 – 637 | SCF-associated factor 1Add BLAST | 637 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 16 | PhosphoserineCombined sources | 1 | |
Modified residuei | 266 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P38352 |
PaxDbi | P38352 |
PRIDEi | P38352 |
PTM databases
iPTMneti | P38352 |
Interactioni
Subunit structurei
Interacts with AAH1, SKP1 and CDC53.
Component of the SCF(SAF1) complex containing CDC53, SKP1, HRT1 and SAF1.
3 PublicationsBinary interactionsi
Hide detailsP38352
With | #Exp. | IntAct |
---|---|---|
AAH1 [P53909] | 2 | EBI-21172,EBI-2197 |
CDC53 [Q12018] | 7 | EBI-21172,EBI-4321 |
HRT1 [Q08273] | 2 | EBI-21172,EBI-31686 |
SKP1 [P52286] | 4 | EBI-21172,EBI-4090 |
Protein-protein interaction databases
BioGRIDi | 32974, 33 interactors |
ComplexPortali | CPX-3254, SCF-Saf1 ubiquitin ligase complex |
DIPi | DIP-6307N |
IntActi | P38352, 7 interactors |
MINTi | P38352 |
STRINGi | 4932.YBR280C |
Miscellaneous databases
RNActi | P38352, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 14 – 63 | F-boxAdd BLAST | 50 | |
Repeati | 109 – 202 | RCC1 1Add BLAST | 94 | |
Repeati | 565 – 635 | RCC1 2Add BLAST | 71 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502QUVE, Eukaryota |
HOGENOMi | CLU_460835_0_0_1 |
InParanoidi | P38352 |
OMAi | ILWHELF |
Family and domain databases
Gene3Di | 2.130.10.30, 2 hits |
InterProi | View protein in InterPro IPR036047, F-box-like_dom_sf IPR009091, RCC1/BLIP-II IPR000408, Reg_chr_condens |
SUPFAMi | SSF50985, SSF50985, 1 hit SSF81383, SSF81383, 1 hit |
PROSITEi | View protein in PROSITE PS00626, RCC1_2, 1 hit PS50012, RCC1_3, 2 hits |
i Sequence
Sequence statusi: Complete.
P38352-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEVESREKE PDAGLSPDIV QATLPFLSSD DIKNLSQTNK YYNTLLDFDH
60 70 80 90 100
SKILWHELFH KAFGTLKTND EPFQGRNSAE FKTCTETILR EAFPTLSWQE
110 120 130 140 150
VYQLRAYDAK FYSWGYLKHG RLGYTASSNN ELVATSLNGP SPRFKYGVNT
160 170 180 190 200
PTEVPWFNSR TTSRTSNFTP SEDPLSAIKK DGDEIIAQVS SGGFSFQILT
210 220 230 240 250
ESGNLYSSGS TFSGGLKGPG PSGSQHDYNP FREMIHNMER SYPRITSRSN
260 270 280 290 300
GSTVNTTGTF SGRRMSGSHP STAYEPGNAT TAQHITIDSG GAPAASPGGS
310 320 330 340 350
HSGVPRTTMP SMGPHENIYS QIEMLERSAN KAVPGNNHIR RMFARNSFPL
360 370 380 390 400
YSGRDENLGS FNDIQFVAVS SGRSHFLAMD TDNNIYSWDS TESDQGVKIE
410 420 430 440 450
FANLPSRATN PILKIASGWN FNCCYIYKVG LVAWKERDAI QKGESFAFAK
460 470 480 490 500
YEIVPNTNDV NGDSRIVDFA CLQDNCVFFI NNNGDKLWKY HNGLNQIVDL
510 520 530 540 550
NIVGKLCKIN ACFASLVLFT DTHCYTLKVT NGDVDKDSLT ELDINENVIS
560 570 580 590 600
VASGDYHTVA LTERGHLYSW GIESQDCGCL GLGPSEKIVN ELHIGNWEGQ
610 620 630
RNIRVVKPTK IELPEDYICV SVTAGGWQTG ALIIKKH
Sequence cautioni
The sequence CAA53643 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA85244 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76053 Genomic DNA Translation: CAA53643.1 Different initiation. Z36149 Genomic DNA Translation: CAA85244.1 Different initiation. BK006936 Genomic DNA Translation: DAA07395.1 |
PIRi | S44542 |
RefSeqi | NP_009839.4, NM_001178628.3 |
Genome annotation databases
EnsemblFungii | YBR280C_mRNA; YBR280C; YBR280C |
GeneIDi | 852583 |
KEGGi | sce:YBR280C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76053 Genomic DNA Translation: CAA53643.1 Different initiation. Z36149 Genomic DNA Translation: CAA85244.1 Different initiation. BK006936 Genomic DNA Translation: DAA07395.1 |
PIRi | S44542 |
RefSeqi | NP_009839.4, NM_001178628.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 32974, 33 interactors |
ComplexPortali | CPX-3254, SCF-Saf1 ubiquitin ligase complex |
DIPi | DIP-6307N |
IntActi | P38352, 7 interactors |
MINTi | P38352 |
STRINGi | 4932.YBR280C |
PTM databases
iPTMneti | P38352 |
Proteomic databases
MaxQBi | P38352 |
PaxDbi | P38352 |
PRIDEi | P38352 |
Genome annotation databases
EnsemblFungii | YBR280C_mRNA; YBR280C; YBR280C |
GeneIDi | 852583 |
KEGGi | sce:YBR280C |
Organism-specific databases
EuPathDBi | FungiDB:YBR280C |
SGDi | S000000484, SAF1 |
Phylogenomic databases
eggNOGi | ENOG502QUVE, Eukaryota |
HOGENOMi | CLU_460835_0_0_1 |
InParanoidi | P38352 |
OMAi | ILWHELF |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:P38352 |
RNActi | P38352, protein |
Family and domain databases
Gene3Di | 2.130.10.30, 2 hits |
InterProi | View protein in InterPro IPR036047, F-box-like_dom_sf IPR009091, RCC1/BLIP-II IPR000408, Reg_chr_condens |
SUPFAMi | SSF50985, SSF50985, 1 hit SSF81383, SSF81383, 1 hit |
PROSITEi | View protein in PROSITE PS00626, RCC1_2, 1 hit PS50012, RCC1_3, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SAF1_YEAST | |
Accessioni | P38352Primary (citable) accession number: P38352 Secondary accession number(s): D6VQS5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | May 16, 2006 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names