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UniProtKB - P38352 (SAF1_YEAST)

Entry version 169 (07 Apr 2021)
Sequence version 2 (16 May 2006)
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Protein

SCF-associated factor 1

Gene

SAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Targets AAH1 adenine deaminase for proteasome-dependent degradation upon entry into quiescence. Targets also URA7.2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00143

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
SCF-associated factor 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SAF1
Ordered Locus Names:YBR280C
ORF Names:YBR2017
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome II

Organism-specific databases

Saccharomyces Genome Database

More...SGDi		S000000484, SAF1

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		FungiDB:YBR280C

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi24L → P in saf1-5; impairs the interaction with SKP1. 1 Publication1
Mutagenesisi35L → P in saf1-6; impairs the interaction with SKP1. 1 Publication1
Mutagenesisi263R → K in saf1-4; impairs the protein stability; when associated with K-264. 1 Publication1
Mutagenesisi264R → K in saf1-4; impairs the protein stability; when associated with K-263. 1 Publication1
Mutagenesisi372G → V in saf1-1; impairs the protein stability. 1 Publication1
Mutagenesisi387S → F in saf1-2; impairs the protein stability. 1 Publication1
Mutagenesisi580L → S in saf1-3; impairs the protein stability. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002066581 – 637SCF-associated factor 1Add BLAST637

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei16PhosphoserineCombined sources1
Modified residuei266PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase

More...MaxQBi		P38352

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P38352

PRoteomics IDEntifications database

More...PRIDEi		P38352

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P38352

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with AAH1, SKP1 and CDC53.

Component of the SCF(SAF1) complex containing CDC53, SKP1, HRT1 and SAF1.

3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		32974, 33 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-3254, SCF-Saf1 ubiquitin ligase complex

Database of interacting proteins

More...DIPi		DIP-6307N

Protein interaction database and analysis system

More...IntActi		P38352, 7 interactors

Molecular INTeraction database

More...MINTi		P38352

STRING: functional protein association networks

More...STRINGi		4932.YBR280C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P38352, protein

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini14 – 63F-boxAdd BLAST50
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati109 – 202RCC1 1Add BLAST94
Repeati565 – 635RCC1 2Add BLAST71

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG502QUVE, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_460835_0_0_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P38352

Identification of Orthologs from Complete Genome Data

More...OMAi		ILWHELF

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.130.10.30, 2 hits

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR036047, F-box-like_dom_sf
IPR009091, RCC1/BLIP-II
IPR000408, Reg_chr_condens

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF50985, SSF50985, 1 hit
SSF81383, SSF81383, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00626, RCC1_2, 1 hit
PS50012, RCC1_3, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P38352-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEVESREKE PDAGLSPDIV QATLPFLSSD DIKNLSQTNK YYNTLLDFDH 
        60         70         80         90        100
SKILWHELFH KAFGTLKTND EPFQGRNSAE FKTCTETILR EAFPTLSWQE 
       110        120        130        140        150
VYQLRAYDAK FYSWGYLKHG RLGYTASSNN ELVATSLNGP SPRFKYGVNT 
       160        170        180        190        200
PTEVPWFNSR TTSRTSNFTP SEDPLSAIKK DGDEIIAQVS SGGFSFQILT 
       210        220        230        240        250
ESGNLYSSGS TFSGGLKGPG PSGSQHDYNP FREMIHNMER SYPRITSRSN 
       260        270        280        290        300
GSTVNTTGTF SGRRMSGSHP STAYEPGNAT TAQHITIDSG GAPAASPGGS 
       310        320        330        340        350
HSGVPRTTMP SMGPHENIYS QIEMLERSAN KAVPGNNHIR RMFARNSFPL 
       360        370        380        390        400
YSGRDENLGS FNDIQFVAVS SGRSHFLAMD TDNNIYSWDS TESDQGVKIE 
       410        420        430        440        450
FANLPSRATN PILKIASGWN FNCCYIYKVG LVAWKERDAI QKGESFAFAK 
       460        470        480        490        500
YEIVPNTNDV NGDSRIVDFA CLQDNCVFFI NNNGDKLWKY HNGLNQIVDL 
       510        520        530        540        550
NIVGKLCKIN ACFASLVLFT DTHCYTLKVT NGDVDKDSLT ELDINENVIS 
       560        570        580        590        600
VASGDYHTVA LTERGHLYSW GIESQDCGCL GLGPSEKIVN ELHIGNWEGQ 
       610        620        630 
RNIRVVKPTK IELPEDYICV SVTAGGWQTG ALIIKKH
Length:637
Mass (Da):70,495
Last modified:May 16, 2006 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i358D751C612744BE
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence CAA53643 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA85244 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X76053 Genomic DNA Translation: CAA53643.1 Different initiation.
Z36149 Genomic DNA Translation: CAA85244.1 Different initiation.
BK006936 Genomic DNA Translation: DAA07395.1

Protein sequence database of the Protein Information Resource

More...PIRi		S44542

NCBI Reference Sequences

More...RefSeqi		NP_009839.4, NM_001178628.3

Genome annotation databases

Ensembl fungal genome annotation project

More...EnsemblFungii		YBR280C_mRNA; YBR280C; YBR280C

Database of genes from NCBI RefSeq genomes

More...GeneIDi		852583

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		sce:YBR280C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76053 Genomic DNA Translation: CAA53643.1 Different initiation.
Z36149 Genomic DNA Translation: CAA85244.1 Different initiation.
BK006936 Genomic DNA Translation: DAA07395.1
PIRiS44542
RefSeqiNP_009839.4, NM_001178628.3

3D structure databases

Database of comparative protein structure models

More...ModBasei		Search...

SWISS-MODEL Interactive Workspace

More...SWISS-MODEL-Workspacei		Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi32974, 33 interactors
ComplexPortaliCPX-3254, SCF-Saf1 ubiquitin ligase complex
DIPiDIP-6307N
IntActiP38352, 7 interactors
MINTiP38352
STRINGi4932.YBR280C

PTM databases

iPTMnetiP38352

Proteomic databases

MaxQBiP38352
PaxDbiP38352
PRIDEiP38352

Genome annotation databases

EnsemblFungiiYBR280C_mRNA; YBR280C; YBR280C
GeneIDi852583
KEGGisce:YBR280C

Organism-specific databases

SGDiS000000484, SAF1
VEuPathDBiFungiDB:YBR280C

Phylogenomic databases

eggNOGiENOG502QUVE, Eukaryota
HOGENOMiCLU_460835_0_0_1
InParanoidiP38352
OMAiILWHELF

Enzyme and pathway databases

UniPathwayiUPA00143

Miscellaneous databases

Protein Ontology

More...PROi		PR:P38352
RNActiP38352, protein

Family and domain databases

Gene3Di2.130.10.30, 2 hits
InterProiView protein in InterPro
IPR036047, F-box-like_dom_sf
IPR009091, RCC1/BLIP-II
IPR000408, Reg_chr_condens
SUPFAMiSSF50985, SSF50985, 1 hit
SSF81383, SSF81383, 1 hit
PROSITEiView protein in PROSITE
PS00626, RCC1_2, 1 hit
PS50012, RCC1_3, 2 hits

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSAF1_YEAST
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P38352
Secondary accession number(s): D6VQS5
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 16, 2006
Last modified: April 7, 2021
This is version 169 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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