UniProtKB - P38344 (REI1_YEAST)
Protein
Cytoplasmic 60S subunit biogenesis factor REI1
Gene
REI1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Pre-60S-associated factor involved in the cytoplasmic maturation of the 60S subunit. Involved in the dissociation and recycling of other late pre-60S factors like ARX1, TIF6 and ALB1 before newly synthesized large ribosomal subunits enter translation. Cooperates with the co-chaperone JJJ1 in the release of the nuclear-export factor ARX1. May act redundantly with REH1 to directly promote a stabilizing structural rearrangement in cytoplasmic 60S subunit maturation independent on ARX1 recycling.7 Publications
Miscellaneous
Present with 830 molecules/cell in log phase SD medium.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 7 – 31 | C2H2-type 1Add BLAST | 25 | |
Zinc fingeri | 162 – 187 | C2H2-type 2Add BLAST | 26 | |
Zinc fingeri | 215 – 239 | C2H2-type 3Add BLAST | 25 |
GO - Molecular functioni
- sequence-specific DNA binding Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- budding cell bud growth Source: SGD
- mitotic cell cycle Source: SGD
- nucleocytoplasmic transport Source: SGD
- ribosomal large subunit biogenesis Source: SGD
Keywordsi
Biological process | Ribosome biogenesis |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Cytoplasmic 60S subunit biogenesis factor REI1Alternative name(s): Required for isotropic bud growth protein 1 pre-60S factor REI1 |
Gene namesi | Name:REI1 Ordered Locus Names:YBR267W ORF Names:YBR1736 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000471, REI1 |
VEuPathDBi | FungiDB:YBR267W |
Subcellular locationi
Other locations
- Cytoplasm 2 Publications
Other locations
- cytoplasm Source: SGD
- preribosome, large subunit precursor Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000202529 | 1 – 393 | Cytoplasmic 60S subunit biogenesis factor REI1Add BLAST | 393 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 140 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P38344 |
PaxDbi | P38344 |
PRIDEi | P38344 |
2D gel databases
SWISS-2DPAGEi | P38344 |
PTM databases
iPTMneti | P38344 |
Interactioni
Subunit structurei
Associates with nascent pre-60S particles that have not yet entered the translating pool, and is released from mature 60S subunits.
Interacts with pre-60S factors ARX1 and RPL24.
Interacts with JJJ1.
4 PublicationsBinary interactionsi
Hide detailsP38344
With | #Exp. | IntAct |
---|---|---|
ARX1 [Q03862] | 5 | EBI-21136,EBI-31385 |
JJJ1 [P53863] | 6 | EBI-21136,EBI-29183 |
LSG1 [P53145] | 5 | EBI-21136,EBI-23885 |
Protein-protein interaction databases
BioGRIDi | 32962, 439 interactors |
DIPi | DIP-6347N |
IntActi | P38344, 13 interactors |
MINTi | P38344 |
STRINGi | 4932.YBR267W |
Miscellaneous databases
RNActi | P38344, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the REI1 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 7 – 31 | C2H2-type 1Add BLAST | 25 | |
Zinc fingeri | 162 – 187 | C2H2-type 2Add BLAST | 26 | |
Zinc fingeri | 215 – 239 | C2H2-type 3Add BLAST | 25 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2785, Eukaryota |
GeneTreei | ENSGT00390000018047 |
HOGENOMi | CLU_018787_1_1_1 |
InParanoidi | P38344 |
OMAi | VANYFIC |
Family and domain databases
InterProi | View protein in InterPro IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR041661, ZN622/Rei1/Reh1_Znf-C2H2 IPR040025, Znf622/Rei1/Reh1 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR13182, PTHR13182, 1 hit |
Pfami | View protein in Pfam PF12756, zf-C2H2_2, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits SM00451, ZnF_U1, 1 hit |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P38344-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSSGVYTCN SCVLTFDSSD EQRAHMKSDW HRYNLKRRVA QLPPISFETF
60 70 80 90 100
DSKVSAAAAS TSKSAEKEKP VTKKELKRRE KQALLEKKKK LLEIARANML
110 120 130 140 150
ENMQKSQEGN TPDLSKLSLQ ENEENKEKEE PKKEEPEQLT EEEMAERVMQ
160 170 180 190 200
EKLRNRVDIP LEQCLFCEHN KHFKDVEENL EHMFRTHGFY IPEQKYLVDK
210 220 230 240 250
IGLVKYMSEK IGLGNICIVC NYQGRTLTAV RQHMLAKRHC KIPYESEDER
260 270 280 290 300
LEISEFYDFT SSYANFNSNT TPDNEDDWED VGSDEAGSDD EDLPQEYLYN
310 320 330 340 350
DGIELHLPTG IKVGHRSLQR YYKQDLKPEV ILTEGQGTLV AAETRSFLPA
360 370 380 390
FDKKGVQTQQ RVWQTERFDK KRLDKRSAKF VNNQPHYRDQ LLQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 152 – 153 | KL → NV in CAA49931 (PubMed:8465606).Curated | 2 | |
Sequence conflicti | 152 – 153 | KL → NV in CAA85229 (PubMed:7813418).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X70529 Genomic DNA Translation: CAA49931.1 Z36135 Genomic DNA Translation: CAA85229.1 BK006936 Genomic DNA Translation: DAA07383.2 |
PIRi | S77691 |
RefSeqi | NP_009825.4, NM_001178615.4 |
Genome annotation databases
EnsemblFungii | YBR267W_mRNA; YBR267W; YBR267W |
GeneIDi | 852569 |
KEGGi | sce:YBR267W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X70529 Genomic DNA Translation: CAA49931.1 Z36135 Genomic DNA Translation: CAA85229.1 BK006936 Genomic DNA Translation: DAA07383.2 |
PIRi | S77691 |
RefSeqi | NP_009825.4, NM_001178615.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5APN | electron microscopy | 3.91 | y | 1-393 | [»] | |
6RZZ | electron microscopy | 3.20 | u | 1-393 | [»] | |
SMRi | P38344 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32962, 439 interactors |
DIPi | DIP-6347N |
IntActi | P38344, 13 interactors |
MINTi | P38344 |
STRINGi | 4932.YBR267W |
PTM databases
iPTMneti | P38344 |
2D gel databases
SWISS-2DPAGEi | P38344 |
Proteomic databases
MaxQBi | P38344 |
PaxDbi | P38344 |
PRIDEi | P38344 |
Genome annotation databases
EnsemblFungii | YBR267W_mRNA; YBR267W; YBR267W |
GeneIDi | 852569 |
KEGGi | sce:YBR267W |
Organism-specific databases
SGDi | S000000471, REI1 |
VEuPathDBi | FungiDB:YBR267W |
Phylogenomic databases
eggNOGi | KOG2785, Eukaryota |
GeneTreei | ENSGT00390000018047 |
HOGENOMi | CLU_018787_1_1_1 |
InParanoidi | P38344 |
OMAi | VANYFIC |
Miscellaneous databases
PROi | PR:P38344 |
RNActi | P38344, protein |
Family and domain databases
InterProi | View protein in InterPro IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR041661, ZN622/Rei1/Reh1_Znf-C2H2 IPR040025, Znf622/Rei1/Reh1 IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR13182, PTHR13182, 1 hit |
Pfami | View protein in Pfam PF12756, zf-C2H2_2, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 3 hits SM00451, ZnF_U1, 1 hit |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS00028, ZINC_FINGER_C2H2_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | REI1_YEAST | |
Accessioni | P38344Primary (citable) accession number: P38344 Secondary accession number(s): D6VQR3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families