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Protein

Alpha N-terminal protein methyltransferase 1

Gene

TAE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Responsible for the N-terminal methylation of the ribosomal proteins RPL12A, RPL12B, RPS25A and RPS25B.1 Publication

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].
2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei76S-adenosyl-L-methionineBy similarity1
Binding sitei139S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • N-terminal protein N-methyltransferase activity Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • N-terminal peptidyl-proline dimethylation Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29185-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha N-terminal protein methyltransferase 1 (EC:2.1.1.244)
Alternative name(s):
Translation associated element 1
X-Pro-Lys N-terminal protein methyltransferase 1
Short name:
NTM1
Gene namesi
Name:TAE1
Synonyms:NTM1
Ordered Locus Names:YBR261C
ORF Names:YBR1729
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR261C
SGDiS000000465 TAE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Defects in both translation efficiency and fidelity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192921 – 232Alpha N-terminal protein methyltransferase 1Add BLAST232

Proteomic databases

MaxQBiP38340
PaxDbiP38340
PRIDEiP38340

PTM databases

iPTMnetiP38340

Interactioni

Protein-protein interaction databases

BioGridi32957, 217 interactors
DIPiDIP-4951N
IntActiP38340, 43 interactors
STRINGi4932.YBR261C

Structurei

3D structure databases

ProteinModelPortaliP38340
SMRiP38340
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 124S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000008371
InParanoidiP38340
KOiK16219
OMAiPVYMIAC
OrthoDBiEOG092C2UR7

Family and domain databases

InterProiView protein in InterPro
IPR008576 MeTrfase_NTM1
IPR029063 SAM-dependent_MTases
PANTHERiPTHR12753 PTHR12753, 1 hit
PfamiView protein in Pfam
PF05891 Methyltransf_PK, 1 hit
PIRSFiPIRSF016958 DUF858_MeTrfase_lik, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

P38340-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDVPADSHIK YEDAIDYWTD VDATVDGVLG GYGEGTVVPT MDVLGSNNFL
60 70 80 90 100
RKLKSRMLPQ ENNVKYAVDI GAGIGRVSKT MLHKHAAKID LVEPVKPFIE
110 120 130 140 150
QMHVELAELK DKGQIGQIYE VGMQDWTPDA GKYWLIWCQW CVGHLPDAEL
160 170 180 190 200
VAFLKRCIVG LQPNGTIVVK ENNTPTDTDD FDETDSSVTR SDAKFRQIFE
210 220 230
EAGLKLIASE RQRGLPRELY PVRMYALKPM PN
Length:232
Mass (Da):26,068
Last modified:October 1, 1994 - v1
Checksum:i66699F37B0013088
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA Translation: CAA49926.1
Z36130 Genomic DNA Translation: CAA85224.1
BK006936 Genomic DNA Translation: DAA07378.1
PIRiS32963
RefSeqiNP_009820.1, NM_001178609.1

Genome annotation databases

EnsemblFungiiYBR261C; YBR261C; YBR261C
GeneIDi852564
KEGGisce:YBR261C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70529 Genomic DNA Translation: CAA49926.1
Z36130 Genomic DNA Translation: CAA85224.1
BK006936 Genomic DNA Translation: DAA07378.1
PIRiS32963
RefSeqiNP_009820.1, NM_001178609.1

3D structure databases

ProteinModelPortaliP38340
SMRiP38340
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32957, 217 interactors
DIPiDIP-4951N
IntActiP38340, 43 interactors
STRINGi4932.YBR261C

PTM databases

iPTMnetiP38340

Proteomic databases

MaxQBiP38340
PaxDbiP38340
PRIDEiP38340

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR261C; YBR261C; YBR261C
GeneIDi852564
KEGGisce:YBR261C

Organism-specific databases

EuPathDBiFungiDB:YBR261C
SGDiS000000465 TAE1

Phylogenomic databases

GeneTreeiENSGT00390000008371
InParanoidiP38340
KOiK16219
OMAiPVYMIAC
OrthoDBiEOG092C2UR7

Enzyme and pathway databases

BioCyciYEAST:G3O-29185-MONOMER

Miscellaneous databases

PROiPR:P38340

Family and domain databases

InterProiView protein in InterPro
IPR008576 MeTrfase_NTM1
IPR029063 SAM-dependent_MTases
PANTHERiPTHR12753 PTHR12753, 1 hit
PfamiView protein in Pfam
PF05891 Methyltransf_PK, 1 hit
PIRSFiPIRSF016958 DUF858_MeTrfase_lik, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNTM1_YEAST
AccessioniPrimary (citable) accession number: P38340
Secondary accession number(s): D6VQQ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 12, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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