UniProtKB - P38313 (CENPU_YEAST)
Inner kinetochore subunit AME1
AME1
Functioni
Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore COMA complex, which connects centromere-associated proteins and the outer kinetochore. COMA interacts with other inner kinetochore proteins to form the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.
1 PublicationMiscellaneous
GO - Biological processi
- attachment of spindle microtubules to kinetochore Source: SGD
- cell division Source: UniProtKB-KW
- meiotic cell cycle Source: UniProtKB-KW
- protein localization to kinetochore Source: SGD
Keywordsi
Biological process | Cell cycle, Cell division, Meiosis, Mitosis |
Names & Taxonomyi
Protein namesi | Recommended name: Inner kinetochore subunit AME1CuratedAlternative name(s): Associated with microtubules and essential protein 1 CENP-U homolog1 Publication Constitutive centromere-associated network protein AME1Curated |
Gene namesi | Name:AME1 Synonyms:ARP100 Ordered Locus Names:YBR211C ORF Names:YBR1458 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000415, AME1 |
VEuPathDBi | FungiDB:YBR211C |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- kinetochore 2 Publications
Cytoskeleton
- spindle pole body Source: SGD
Nucleus
- COMA complex Source: SGD
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
- kinetochore Source: SGD
Keywords - Cellular componenti
Centromere, Chromosome, Kinetochore, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064578 | 1 – 324 | Inner kinetochore subunit AME1Add BLAST | 324 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 41 | PhosphoserineCombined sources | 1 | |
Modified residuei | 45 | PhosphoserineCombined sources | 1 | |
Modified residuei | 53 | PhosphoserineCombined sources | 1 | |
Modified residuei | 59 | PhosphoserineCombined sources | 1 | |
Modified residuei | 101 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P38313 |
PaxDbi | P38313 |
PRIDEi | P38313 |
PTM databases
iPTMneti | P38313 |
Interactioni
Subunit structurei
Component of the heterotetrameric kinetochore subcomplex COMA, which consists of AME1, CTF19, MCM21 and OKP1 (PubMed:14633972). The COMA subcomplex is part of a larger constitutive centromere-associated network (CCAN) (also known as central kinetochore CTF19 complex in yeast), which is composed of at least AME1, CHL4, CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2, OKP1 and WIP1 (PubMed:12408861, PubMed:22561346). COMA binds the centromeric nucleosome-binding protein MIF2, and to the outer kinetochore MIND subcomplex, probably via AME1. AME1 interacts directly with OKP1 and an NKP1-NKP2 dimer (By similarity).
By similarity3 PublicationsProtein-protein interaction databases
BioGRIDi | 32908, 299 interactors |
ComplexPortali | CPX-1187, COMA complex |
DIPi | DIP-1588N |
IntActi | P38313, 9 interactors |
MINTi | P38313 |
STRINGi | 4932.YBR211C |
Miscellaneous databases
RNActi | P38313, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P38313 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 199 – 263 | Sequence analysisAdd BLAST | 65 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502S0ZV, Eukaryota |
HOGENOMi | CLU_068947_0_0_1 |
InParanoidi | P38313 |
OMAi | HFVDLMD |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDRDTKLAFR LRGSHSRRTD DIDDDVIVFK TPNAVYREEN SPIQSPVQPI
60 70 80 90 100
LSSPKLANSF EFPITTNNVN AQDRHEHGYQ PLDAEDYPMI DSENKSLISE
110 120 130 140 150
SPQNVRNDED LTTRYNFDDI PIRQLSSSIT SVTTIDVLSS LFINLFENDL
160 170 180 190 200
IPQALKDFNK SDDDQFRKLL YKLDLRLFQT ISDQMTRDLK DILDINVSNN
210 220 230 240 250
ELCYQLKQVL ARKEDLNQQI ISVRNEIQEL KAGKDWHDLQ NEQAKLNDKV
260 270 280 290 300
KLNKRLNDLT STLLGKYEGD RKIMSQDSED DSIRDDSNIL DIAHFVDLMD
310 320
PYNGLLKKIN KINENLSNEL QPSL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36080 Genomic DNA Translation: CAA85175.1 AY692799 Genomic DNA Translation: AAT92818.1 BK006936 Genomic DNA Translation: DAA07327.1 |
PIRi | S46085 |
RefSeqi | NP_009770.3, NM_001178559.3 |
Genome annotation databases
EnsemblFungii | YBR211C_mRNA; YBR211C; YBR211C |
GeneIDi | 852512 |
KEGGi | sce:YBR211C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z36080 Genomic DNA Translation: CAA85175.1 AY692799 Genomic DNA Translation: AAT92818.1 BK006936 Genomic DNA Translation: DAA07327.1 |
PIRi | S46085 |
RefSeqi | NP_009770.3, NM_001178559.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6NUW | electron microscopy | 4.25 | I | 1-324 | [»] | |
6QLD | electron microscopy | 4.15 | U | 131-320 | [»] | |
6QLE | electron microscopy | 3.55 | U | 166-324 | [»] | |
6QLF | electron microscopy | 3.45 | U | 1-320 | [»] | |
SMRi | P38313 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32908, 299 interactors |
ComplexPortali | CPX-1187, COMA complex |
DIPi | DIP-1588N |
IntActi | P38313, 9 interactors |
MINTi | P38313 |
STRINGi | 4932.YBR211C |
PTM databases
iPTMneti | P38313 |
Proteomic databases
MaxQBi | P38313 |
PaxDbi | P38313 |
PRIDEi | P38313 |
Genome annotation databases
EnsemblFungii | YBR211C_mRNA; YBR211C; YBR211C |
GeneIDi | 852512 |
KEGGi | sce:YBR211C |
Organism-specific databases
SGDi | S000000415, AME1 |
VEuPathDBi | FungiDB:YBR211C |
Phylogenomic databases
eggNOGi | ENOG502S0ZV, Eukaryota |
HOGENOMi | CLU_068947_0_0_1 |
InParanoidi | P38313 |
OMAi | HFVDLMD |
Miscellaneous databases
PROi | PR:P38313 |
RNActi | P38313, protein |
Family and domain databases
MobiDBi | Search... |
Entry informationi
Entry namei | CENPU_YEAST | |
Accessioni | P38313Primary (citable) accession number: P38313 Secondary accession number(s): D6VQK7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | September 29, 2021 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families