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Protein

Exonuclease V, mitochondrial

Gene

EXO5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Single strand DNA specific 5' exonuclease involved in mitochondrial DNA replication and recombination. Releases dinucleotides as main products of catalysis. Has the capacity to slide across 5'double-stranded DNA or 5'RNA sequences and resumes cutting two nucleotides downstream of the double-stranded-to-single-stranded junction or RNA-to-DNA junction, respectively.3 Publications

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+1 Publication
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

pH dependencei

Optimum pH is 7.8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi141Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi549Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi552Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi558Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • mitochondrial genome maintenance Source: SGD

Keywordsi

Molecular functionDNA-binding, Exonuclease, Hydrolase, Nuclease
Ligand4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29113-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Exonuclease V, mitochondrial (EC:3.1.-.-)
Short name:
Exo V
Alternative name(s):
Defects in morphology protein 1
Gene namesi
Name:EXO5
Synonyms:DEM1
Ordered Locus Names:YBR163W
ORF Names:YBR1215
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR163W
SGDiS000000367 EXO5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi270D → A: Impairs exonuclease activity. 1 Publication1
Mutagenesisi320D → A: Impairs exonuclease activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionSequence analysisAdd BLAST26
ChainiPRO_000020250127 – 585Exonuclease V, mitochondrialAdd BLAST559

Proteomic databases

MaxQBiP38289
PaxDbiP38289
PRIDEiP38289

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi32863, 32 interactors
IntActiP38289, 3 interactors
MINTiP38289
STRINGi4932.YBR163W

Structurei

3D structure databases

ProteinModelPortaliP38289
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the EXO5 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiP38289
KOiK17815
OMAiSSWCELR
OrthoDBiEOG092C160Q

Family and domain databases

InterProiView protein in InterPro
IPR016610 Exo5
IPR019190 EXOV
PfamiView protein in Pfam
PF09810 Exo5, 1 hit
PIRSFiPIRSF013220 UCP013220, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGRALINKY GFLIHPRRFV HLNDKSLDGT FILPSKKNHM YDVPTNDPSG
60 70 80 90 100
ILNASDIDRI NNLPFFDNTS PTKETNTKEG ALLSEKLASV KELFGEDPEN
110 120 130 140 150
PSFINYRFPR GLENPYFDIQ VNQLKKKRLS VTQLCTTQNW CELRNFYDFY
160 170 180 190 200
SQNLSNQLLN LKFQVQKGKK IHKSLEDETH PELNQYKSFT HNFLALTKLS
210 220 230 240 250
MDIDNDMDAL LDNWFNSINR LVSLFTKGDG HAREIVCHGF INLEDGKLVE
260 270 280 290 300
HLLNSDSKTK ENVIISGVID HLTLRNRHNH QVQKGAAHLD TEYQSWGNIL
310 320 330 340 350
TNLLSNLKEL KSNNEIVISD IKTRSVPKIP SIESVIESSK LQTMYYKFFF
360 370 380 390 400
SHLSQDMTQT YHSFLINAQR RGLDVDAPIN PTKILTFILT NPLFANDVKN
410 420 430 440 450
LLYGLPINHS AFDNDAKGSN TFDMTAFNDL LDRGPTSFNV PIEQDEDSSE
460 470 480 490 500
STKCVSLRDY GHFYTKWKTP LTLKYFAARL SQIYFIVGNL VSNDLMIEYY
510 520 530 540 550
YHNDNFHNII FPYDPLKLGT HAHDSAMVWF GGRDMHPIEP TQKNFNTYCK
560 570 580
FCDYRHVCSW KNKNELKLID LGKELKKIIL ESSMK
Length:585
Mass (Da):67,570
Last modified:October 1, 1994 - v1
Checksum:i92DBE35B819E0AFB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36032 Genomic DNA Translation: CAA85123.1
BK006936 Genomic DNA Translation: DAA07279.1
PIRiS46034
RefSeqiNP_009722.3, NM_001178511.3

Genome annotation databases

EnsemblFungiiYBR163W; YBR163W; YBR163W
GeneIDi852461
KEGGisce:YBR163W

Similar proteinsi

Entry informationi

Entry nameiEXO5_YEAST
AccessioniPrimary (citable) accession number: P38289
Secondary accession number(s): D6VQF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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