UniProtKB - P38251 (RFC5_YEAST)
Protein
Replication factor C subunit 5
Gene
RFC5
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair.5 Publications
Miscellaneous
Present with 5040 molecules/cell in log phase SD medium.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 5 | ATP; via carbonyl oxygen | 1 | |
Binding sitei | 17 | ATP | 1 | |
Binding sitei | 231 | ATP | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 43 – 51 | ATP | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- DNA-dependent DNA replication Source: GO_Central
- DNA repair Source: GO_Central
- leading strand elongation Source: SGD
- sister chromatid cohesion Source: SGD
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-176187, Activation of ATR in response to replication stress R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-69091, Polymerase switching |
Names & Taxonomyi
Protein namesi | Recommended name: Replication factor C subunit 5Short name: Replication factor C5 Alternative name(s): Activator 1 40 kDa subunit |
Gene namesi | Name:RFC5 Ordered Locus Names:YBR087W ORF Names:YBR0810 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YBR087W |
SGDi | S000000291, RFC5 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: GO_Central
Other locations
- Ctf18 RFC-like complex Source: SGD
- DNA replication factor C complex Source: SGD
- Elg1 RFC-like complex Source: SGD
- Rad17 RFC-like complex Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121765 | 1 – 354 | Replication factor C subunit 5Add BLAST | 354 |
Proteomic databases
MaxQBi | P38251 |
PaxDbi | P38251 |
PRIDEi | P38251 |
PTM databases
iPTMneti | P38251 |
Interactioni
Subunit structurei
Replication factor C (RFC) is a heteropentamer of subunits RFC1, RFC2, RFC3, RFC4 and RFC5 and forms a complex with POL30/PCNA in the presence of ATP.
Component of the RAD24-RFC complex which consists of RAD24, RFC2, RFC3, RFC4 and RFC5 and associates with the checkpoint clamp DDC1:MEC3:RAD17 complex.
Component of the ELG1-RFC complex which consists of ELG1, RFC2, RFC3, RFC4 and RFC5.
Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. RFC5 interacts with ECO1.
9 PublicationsBinary interactionsi
Hide detailsP38251
With | #Exp. | IntAct |
---|---|---|
CTF18 [P49956] | 4 | EBI-15016,EBI-4560 |
CTF8 [P38877] | 2 | EBI-15016,EBI-5216 |
ELG1 [Q12050] | 4 | EBI-15016,EBI-32195 |
RAD24 [P32641] | 4 | EBI-15016,EBI-14675 |
Protein-protein interaction databases
BioGRIDi | 32793, 292 interactors |
ComplexPortali | CPX-1731, CTF18-RFC complex CPX-1807, Rad17 RFC-like complex CPX-422, ELG1-RFC complex CPX-545, DNA replication factor C complex |
DIPi | DIP-2531N |
IntActi | P38251, 15 interactors |
MINTi | P38251 |
STRINGi | 4932.YBR087W |
Miscellaneous databases
RNActi | P38251, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P38251 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P38251 |
Family & Domainsi
Sequence similaritiesi
Belongs to the activator 1 small subunits family.Curated
Phylogenomic databases
eggNOGi | KOG2035, Eukaryota |
GeneTreei | ENSGT00550000075006 |
HOGENOMi | CLU_042324_5_0_1 |
InParanoidi | P38251 |
OMAi | WVVKVMS |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR008921, DNA_pol3_clamp-load_cplx_C IPR027417, P-loop_NTPase |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52540, SSF52540, 1 hit |
i Sequence
Sequence statusi: Complete.
P38251-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLWVDKYRP KSLNALSHNE ELTNFLKSLS DQPRDLPHLL LYGPNGTGKK
60 70 80 90 100
TRCMALLESI FGPGVYRLKI DVRQFVTASN RKLELNVVSS PYHLEITPSD
110 120 130 140 150
MGNNDRIVIQ ELLKEVAQME QVDFQDSKDG LAHRYKCVII NEANSLTKDA
160 170 180 190 200
QAALRRTMEK YSKNIRLIMV CDSMSPIIAP IKSRCLLIRC PAPSDSEIST
210 220 230 240 250
ILSDVVTNER IQLETKDILK RIAQASNGNL RVSLLMLESM ALNNELALKS
260 270 280 290 300
SSPIIKPDWI IVIHKLTRKI VKERSVNSLI ECRAVLYDLL AHCIPANIIL
310 320 330 340 350
KELTFSLLDV ETLNTTNKSS IIEYSSVFDE RLSLGNKAIF HLEGFIAKVM
CCLD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U26031 Genomic DNA Translation: AAC49065.1 X78993 Genomic DNA Translation: CAA55595.1 Z35956 Genomic DNA Translation: CAA85036.1 AY693173 Genomic DNA Translation: AAT93192.1 BK006936 Genomic DNA Translation: DAA07208.1 |
PIRi | S48257 |
RefSeqi | NP_009644.3, NM_001178435.3 |
Genome annotation databases
EnsemblFungii | YBR087W_mRNA; YBR087W; YBR087W |
GeneIDi | 852383 |
KEGGi | sce:YBR087W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U26031 Genomic DNA Translation: AAC49065.1 X78993 Genomic DNA Translation: CAA55595.1 Z35956 Genomic DNA Translation: CAA85036.1 AY693173 Genomic DNA Translation: AAT93192.1 BK006936 Genomic DNA Translation: DAA07208.1 |
PIRi | S48257 |
RefSeqi | NP_009644.3, NM_001178435.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SXJ | X-ray | 2.85 | E | 1-354 | [»] | |
SMRi | P38251 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32793, 292 interactors |
ComplexPortali | CPX-1731, CTF18-RFC complex CPX-1807, Rad17 RFC-like complex CPX-422, ELG1-RFC complex CPX-545, DNA replication factor C complex |
DIPi | DIP-2531N |
IntActi | P38251, 15 interactors |
MINTi | P38251 |
STRINGi | 4932.YBR087W |
PTM databases
iPTMneti | P38251 |
Proteomic databases
MaxQBi | P38251 |
PaxDbi | P38251 |
PRIDEi | P38251 |
Genome annotation databases
EnsemblFungii | YBR087W_mRNA; YBR087W; YBR087W |
GeneIDi | 852383 |
KEGGi | sce:YBR087W |
Organism-specific databases
EuPathDBi | FungiDB:YBR087W |
SGDi | S000000291, RFC5 |
Phylogenomic databases
eggNOGi | KOG2035, Eukaryota |
GeneTreei | ENSGT00550000075006 |
HOGENOMi | CLU_042324_5_0_1 |
InParanoidi | P38251 |
OMAi | WVVKVMS |
Enzyme and pathway databases
Reactomei | R-SCE-176187, Activation of ATR in response to replication stress R-SCE-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-SCE-69091, Polymerase switching |
Miscellaneous databases
EvolutionaryTracei | P38251 |
PROi | PR:P38251 |
RNActi | P38251, protein |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR008921, DNA_pol3_clamp-load_cplx_C IPR027417, P-loop_NTPase |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF48019, SSF48019, 1 hit SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RFC5_YEAST | |
Accessioni | P38251Primary (citable) accession number: P38251 Secondary accession number(s): D6VQ88 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 174 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names