UniProtKB - P38219 (OLA1_YEAST)
Protein
Obg-like ATPase 1
Gene
OLA1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.UniRule annotation1 Publication
Miscellaneous
Present with 36803 molecules/cell in log phase SD medium.1 Publication
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 34 | MagnesiumBy similarity | 1 | |
Metal bindingi | 55 | MagnesiumBy similarity | 1 | |
Binding sitei | 233 | ATP; via carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 30 – 35 | ATPUniRule annotation | 6 |
GO - Molecular functioni
- ATPase activity Source: SGD
- ATP binding Source: UniProtKB-UniRule
- GTP binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- ribosomal large subunit binding Source: UniProtKB-UniRule
- ribosome binding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-114608, Platelet degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Obg-like ATPase 1UniRule annotation |
Gene namesi | Name:OLA1UniRule annotation Ordered Locus Names:YBR025C ORF Names:YBR0309 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YBR025C |
SGDi | S000000229, OLA1 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122462 | 1 – 394 | Obg-like ATPase 1Add BLAST | 394 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 89 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 98 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources | ||
Modified residuei | 116 | PhosphoserineCombined sources | 1 | |
Modified residuei | 119 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P38219 |
PaxDbi | P38219 |
PRIDEi | P38219 |
PTM databases
CarbonylDBi | P38219 |
iPTMneti | P38219 |
Expressioni
Inductioni
By hydrogen peroxide and during DNA replication stress.2 Publications
Interactioni
Subunit structurei
Monomer (By similarity).
Interacts with the 26S proteasome subunit RPT6.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 32728, 158 interactors |
DIPi | DIP-4893N |
IntActi | P38219, 52 interactors |
MINTi | P38219 |
STRINGi | 4932.YBR025C |
Miscellaneous databases
RNActi | P38219, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 285 | OBG-type GAdd BLAST | 265 | |
Domaini | 306 – 389 | TGSPROSITE-ProRule annotationAdd BLAST | 84 |
Sequence similaritiesi
Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | KOG1491, Eukaryota |
GeneTreei | ENSGT00390000000673 |
HOGENOMi | CLU_018395_1_2_1 |
InParanoidi | P38219 |
OMAi | VLRCFDN |
Family and domain databases
CDDi | cd04867, TGS_YchF_OLA1, 1 hit cd01900, YchF, 1 hit |
Gene3Di | 1.10.150.300, 1 hit 3.10.20.30, 1 hit |
HAMAPi | MF_00944, YchF_OLA1_ATPase, 1 hit |
InterProi | View protein in InterPro IPR004396, ATPase_YchF/OLA1 IPR012675, Beta-grasp_dom_sf IPR031167, G_OBG IPR006073, GTP_binding_domain IPR027417, P-loop_NTPase IPR004095, TGS IPR012676, TGS-like IPR023192, TGS-like_dom_sf IPR013029, YchF_C IPR041706, YchF_N |
Pfami | View protein in Pfam PF01926, MMR_HSR1, 1 hit PF06071, YchF-GTPase_C, 1 hit |
PIRSFi | PIRSF006641, CHP00092, 1 hit |
PRINTSi | PR00326, GTP1OBG |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF81271, SSF81271, 1 hit |
TIGRFAMsi | TIGR00092, TIGR00092, 1 hit |
PROSITEi | View protein in PROSITE PS51710, G_OBG, 1 hit PS51880, TGS, 1 hit |
i Sequence
Sequence statusi: Complete.
P38219-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPPKKQVEEK KVLLGRPGNN LKAGIVGLAN VGKSTFFQAI TRCPLGNPAN
60 70 80 90 100
YPFATIDPEE ARVIVPSPRF DKLCEIYKKT ASEVPAHLTV YDIAGLTKGA
110 120 130 140 150
SAGEGLGNAF LSHIRSVDSI YQVVRCFDDA EIIHVEGDVD PVRDLEIINQ
160 170 180 190 200
ELRLKDIEFA QKALEGAEKI AKRGGQSLEV KQKKEEMDLI TKIIKLLESG
210 220 230 240 250
QRVANHSWTS KEVEIINSMF LLTAKPCIYL INLSERDYIR KKNKHLLRIK
260 270 280 290 300
EWVDKYSPGD LIIPFSVSLE ERLSHMSPED AEEELKKLQT ISALPKIITT
310 320 330 340 350
MRQKLDLISF FTCGPDEVRE WTIRRGTKAP QAAGVIHNDL MNTFILAQVM
360 370 380 390
KCEDVFEYKD DSAIKAAGKL MQKGKDYVVE DGDIIYFRAG AGKN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76078 Genomic DNA Translation: CAA53682.1 Z35894 Genomic DNA Translation: CAA84967.1 BK006936 Genomic DNA Translation: DAA07147.1 |
PIRi | S45881 |
RefSeqi | NP_009581.1, NM_001178373.1 |
Genome annotation databases
EnsemblFungii | YBR025C_mRNA; YBR025C; YBR025C |
GeneIDi | 852313 |
KEGGi | sce:YBR025C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X76078 Genomic DNA Translation: CAA53682.1 Z35894 Genomic DNA Translation: CAA84967.1 BK006936 Genomic DNA Translation: DAA07147.1 |
PIRi | S45881 |
RefSeqi | NP_009581.1, NM_001178373.1 |
3D structure databases
SMRi | P38219 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32728, 158 interactors |
DIPi | DIP-4893N |
IntActi | P38219, 52 interactors |
MINTi | P38219 |
STRINGi | 4932.YBR025C |
PTM databases
CarbonylDBi | P38219 |
iPTMneti | P38219 |
Proteomic databases
MaxQBi | P38219 |
PaxDbi | P38219 |
PRIDEi | P38219 |
Genome annotation databases
EnsemblFungii | YBR025C_mRNA; YBR025C; YBR025C |
GeneIDi | 852313 |
KEGGi | sce:YBR025C |
Organism-specific databases
EuPathDBi | FungiDB:YBR025C |
SGDi | S000000229, OLA1 |
Phylogenomic databases
eggNOGi | KOG1491, Eukaryota |
GeneTreei | ENSGT00390000000673 |
HOGENOMi | CLU_018395_1_2_1 |
InParanoidi | P38219 |
OMAi | VLRCFDN |
Enzyme and pathway databases
Reactomei | R-SCE-114608, Platelet degranulation |
Miscellaneous databases
PROi | PR:P38219 |
RNActi | P38219, protein |
Family and domain databases
CDDi | cd04867, TGS_YchF_OLA1, 1 hit cd01900, YchF, 1 hit |
Gene3Di | 1.10.150.300, 1 hit 3.10.20.30, 1 hit |
HAMAPi | MF_00944, YchF_OLA1_ATPase, 1 hit |
InterProi | View protein in InterPro IPR004396, ATPase_YchF/OLA1 IPR012675, Beta-grasp_dom_sf IPR031167, G_OBG IPR006073, GTP_binding_domain IPR027417, P-loop_NTPase IPR004095, TGS IPR012676, TGS-like IPR023192, TGS-like_dom_sf IPR013029, YchF_C IPR041706, YchF_N |
Pfami | View protein in Pfam PF01926, MMR_HSR1, 1 hit PF06071, YchF-GTPase_C, 1 hit |
PIRSFi | PIRSF006641, CHP00092, 1 hit |
PRINTSi | PR00326, GTP1OBG |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF81271, SSF81271, 1 hit |
TIGRFAMsi | TIGR00092, TIGR00092, 1 hit |
PROSITEi | View protein in PROSITE PS51710, G_OBG, 1 hit PS51880, TGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OLA1_YEAST | |
Accessioni | P38219Primary (citable) accession number: P38219 Secondary accession number(s): D6VQ27 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1994 |
Last sequence update: | October 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 181 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names