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Protein

Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase

Gene

ALG3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adds the first Dol-P-Man derived mannose in an alpha-1,3 linkage to Man5GlcNAc2-PP-Dol. Sensitive to H.mrakii HM-1 killer toxin.2 Publications

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,3-mannosyltransferase activity Source: SGD
  • dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YBL082C-MONOMER
YEAST:YBL082C-MONOMER
BRENDAi2.4.1.258 984
ReactomeiR-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGT58 Glycosyltransferase Family 58

Names & Taxonomyi

Protein namesi
Recommended name:
Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase (EC:2.4.1.258)
Alternative name(s):
Asparagine-linked glycosylation protein 3
Dol-P-Man-dependent alpha(1-3)-mannosyltransferase
Dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase
Dolichyl-phosphate-mannose--glycolipid alpha-mannosyltransferase
HM-1 killer toxin resistance protein
Gene namesi
Name:ALG3
Synonyms:RHK1
Ordered Locus Names:YBL082C
ORF Names:YBL0720
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL082C
SGDiS000000178 ALG3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 41LumenalSequence analysisAdd BLAST41
Transmembranei42 – 62HelicalSequence analysisAdd BLAST21
Topological domaini63 – 95CytoplasmicSequence analysisAdd BLAST33
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 137LumenalSequence analysisAdd BLAST21
Transmembranei138 – 158HelicalSequence analysisAdd BLAST21
Topological domaini159 – 171CytoplasmicSequence analysisAdd BLAST13
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Topological domaini193 – 216LumenalSequence analysisAdd BLAST24
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Topological domaini238 – 242CytoplasmicSequence analysis5
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 302LumenalSequence analysisAdd BLAST39
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Topological domaini324 – 345CytoplasmicSequence analysisAdd BLAST22
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Topological domaini367 – 381LumenalSequence analysisAdd BLAST15
Transmembranei382 – 402HelicalSequence analysisAdd BLAST21
Topological domaini403 – 415CytoplasmicSequence analysisAdd BLAST13
Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
Topological domaini437 – 458LumenalSequence analysisAdd BLAST22

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi275A → V in ALG3-1; leads to an underglycosylation of secretory proteins. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805651 – 458Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferaseAdd BLAST458

Proteomic databases

MaxQBiP38179
PaxDbiP38179
PRIDEiP38179

Interactioni

Protein-protein interaction databases

BioGridi32621, 178 interactors
DIPiDIP-4854N
MINTiP38179
STRINGi4932.YBL082C

Structurei

3D structure databases

ProteinModelPortaliP38179
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 58 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000013904
HOGENOMiHOG000237555
InParanoidiP38179
KOiK03845
OMAiTKKVPPY
OrthoDBiEOG092C26UZ

Family and domain databases

InterProiView protein in InterPro
IPR007873 Glycosyltransferase_ALG3
PANTHERiPTHR12646 PTHR12646, 1 hit
PfamiView protein in Pfam
PF05208 ALG3, 1 hit

Sequencei

Sequence statusi: Complete.

P38179-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEGEQSPQGE KSLQRKQFVR PPLDLWQDLK DGVRYVIFDC RANLIVMPLL
60 70 80 90 100
ILFESMLCKI IIKKVAYTEI DYKAYMEQIE MIQLDGMLDY SQVSGGTGPL
110 120 130 140 150
VYPAGHVLIY KMMYWLTEGM DHVERGQVFF RYLYLLTLAL QMACYYLLHL
160 170 180 190 200
PPWCVVLACL SKRLHSIYVL RLFNDCFTTL FMVVTVLGAI VASRCHQRPK
210 220 230 240 250
LKKSLALVIS ATYSMAVSIK MNALLYFPAM MISLFILNDA NVILTLLDLV
260 270 280 290 300
AMIAWQVAVA VPFLRSFPQQ YLHCAFNFGR KFMYQWSINW QMMDEEAFND
310 320 330 340 350
KRFHLALLIS HLIALTTLFV TRYPRILPDL WSSLCHPLRK NAVLNANPAK
360 370 380 390 400
TIPFVLIASN FIGVLFSRSL HYQFLSWYHW TLPILIFWSG MPFFVGPIWY
410 420 430 440 450
VLHEWCWNSY PPNSQASTLL LALNTVLLLL LALTQLSGSV ALAKSHLRTT

SSMEKKLN
Length:458
Mass (Da):52,861
Last modified:October 1, 1994 - v1
Checksum:i1C2FEAD6D459C249
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86G → R in AAA75352 (PubMed:7754714).Curated1
Sequence conflicti187 – 198LGAIV…RCHQR → FRGYRGQQVPSA in AAA75352 (PubMed:7754714).CuratedAdd BLAST12
Sequence conflicti266S → Q in AAA75352 (PubMed:7754714).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA Translation: CAA56024.1
Z35844 Genomic DNA Translation: CAA84904.1
M89908 Genomic DNA Translation: AAA75352.1
BK006936 Genomic DNA Translation: DAA07041.1
PIRiS45424
RefSeqiNP_009471.1, NM_001178322.1

Genome annotation databases

EnsemblFungiiYBL082C; YBL082C; YBL082C
GeneIDi852196
KEGGisce:YBL082C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA Translation: CAA56024.1
Z35844 Genomic DNA Translation: CAA84904.1
M89908 Genomic DNA Translation: AAA75352.1
BK006936 Genomic DNA Translation: DAA07041.1
PIRiS45424
RefSeqiNP_009471.1, NM_001178322.1

3D structure databases

ProteinModelPortaliP38179
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32621, 178 interactors
DIPiDIP-4854N
MINTiP38179
STRINGi4932.YBL082C

Protein family/group databases

CAZyiGT58 Glycosyltransferase Family 58

Proteomic databases

MaxQBiP38179
PaxDbiP38179
PRIDEiP38179

Protocols and materials databases

DNASUi852196
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL082C; YBL082C; YBL082C
GeneIDi852196
KEGGisce:YBL082C

Organism-specific databases

EuPathDBiFungiDB:YBL082C
SGDiS000000178 ALG3

Phylogenomic databases

GeneTreeiENSGT00390000013904
HOGENOMiHOG000237555
InParanoidiP38179
KOiK03845
OMAiTKKVPPY
OrthoDBiEOG092C26UZ

Enzyme and pathway databases

UniPathwayi
UPA00378

BioCyciMetaCyc:YBL082C-MONOMER
YEAST:YBL082C-MONOMER
BRENDAi2.4.1.258 984
ReactomeiR-SCE-446193 Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein

Miscellaneous databases

PROiPR:P38179

Family and domain databases

InterProiView protein in InterPro
IPR007873 Glycosyltransferase_ALG3
PANTHERiPTHR12646 PTHR12646, 1 hit
PfamiView protein in Pfam
PF05208 ALG3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiALG3_YEAST
AccessioniPrimary (citable) accession number: P38179
Secondary accession number(s): D6VPS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 10, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names
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Main funding by: National Institutes of Health

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